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Full-Text Articles in Chemistry
Determination Of Mln0128, An Investigational Antineoplastic Agent, In Human Plasma By Lc–Ms/Ms, Sandeep R. Kunati, Yan Xu
Determination Of Mln0128, An Investigational Antineoplastic Agent, In Human Plasma By Lc–Ms/Ms, Sandeep R. Kunati, Yan Xu
Chemistry Faculty Publications
MLN0128, an mTOR kinase inhibitor, is currently undergoing clinical investigation for treatment of a variety of cancers. To support this work, an LC–MS/MS method has been developed for the determination of MLN0128 in human plasma. A structural analog STK040263 was used as the internal standard. Both MLN0128 and the IS were first extracted from plasma using methyl tert-butyl ether; then separated on a Waters XTerra® MS C18 column using a mobile phase consisting of methanol–acetonitrile–10.0 mm ammonium formate (34:6:60, v/v/v) at a flow rate of 0.300 mL min−1. Quantitation of MLN0128 was done by positive electrospray ionization tandem mass spectrometry …
Globally Profiling Sialylation Status Of Macrophages Upon Statin Treatment, Dan Wang, Huan Nie, Evgeny Ozhegov, Lin Wang, Aimin Zhou, Yu Li, Xue Long Sun
Globally Profiling Sialylation Status Of Macrophages Upon Statin Treatment, Dan Wang, Huan Nie, Evgeny Ozhegov, Lin Wang, Aimin Zhou, Yu Li, Xue Long Sun
Chemistry Faculty Publications
Sialic acids (SAs) are widely expressed on immune cells and their levels and linkages named as sialylation status vary upon cellular environment changes related to both physiological and pathological processes. In this study, we performed a global profiling of the sialylation status of macrophages and their release of SAs in the cell culture medium by using flow cytometry, confocal microscopy and liquid chromatography tandem mass spectrometry (LC-MS/MS). Both flow cytometry and confocal microscopy results showed that cell surface α-2,3-linked SAs were predominant in the normal culture condition and changed slightly upon treatment with atorvastatin for 24 h, whereas α-2,6-linked SAs …