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Understanding The Effects Of Temperature On Electrostatic Dna Denaturation, Jeffrey Taylor
Understanding The Effects Of Temperature On Electrostatic Dna Denaturation, Jeffrey Taylor
Master's Theses
In this work, the electrostatic denaturation of 18-bp DNA duplexes was investigated via Square Wave Voltammetry (SWV) to determine the extent of melting (∆) and rate of melting (𝝉). Electrostatic denaturation was observed over the temperature range of 20-50 °C, and purely electrostatic melting was defined from 27.5°C. In contrast, electrostatic denaturation for 30 °C was defined as a mixture of thermal melting thiol desorption + electrostatic melting. Electrostatic denaturation is a temperature-dependent process, and the amount of melting of the dsDNA increased with temperature. It was also observed that the ∆ and 𝝉 values increase with temperature further …