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Binding Interactions Between Mixed-Monlayer Functionalized Gold Nanoparticles (Aunps) And The Serum Protein Albumin, Jennifer Hanigan-Diebel
Binding Interactions Between Mixed-Monlayer Functionalized Gold Nanoparticles (Aunps) And The Serum Protein Albumin, Jennifer Hanigan-Diebel
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This study investigated the protein adsorption interactions of bovine serum albumin (BSA) with three different functionalized 5 nm gold nanoparticles (AuNPs) in order to determine the binding affinity and total amount of protein adsorbed to each AuNP surface chemistry. AuNPs were synthesized using two different capping agents to display three different surface chemistries: a neutral ω-functionalized thiol ligand (mercapto-ethoxy-ethoxy-ethanol, MEEE), a thiol ligand that is negatively charged at pH 7.4 (mercaptohexanoic acid, MHA), and a mixture of the two ligands (mixed-ligand AuNPs). The interactions of this library of AuNPs with bovine serum albumin (BSA) were investigated using UV-visible absorbance spectroscopy, …