Chemistry Commons^™

Novel Modulation Of Adenylyl Cyclase Type 2, Jason Michael Conley

Open Access Dissertations

Adenylyl cyclase isoforms are distinctly modulated by G protein subunits and are therefore hypothesized to be uniquely regulated by proteins that influence G protein signaling. Activator of G protein signaling 3 (AGS3) is a G protein modulator that binds Gαi subunits in the GDP-bound state, implicating AGS3 as an important regulatorof Gi-coupled receptor signaling. We studied the ability of AGS3 to modulate recombinant adenylyl cyclase(AC) type 1 and 2 signaling in HEK293 cells following both acute and persistent activation of the D _2Ldopamine receptor (D_2L DR). AGS3 expression modestly enhanced the potency of acute quinpirole-induced D _2LDR …