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Full-Text Articles in Chemistry
Evaluation Of Ispe For Inhibitor Design In The Non-Mevalonate Pathway, Kataryzna B. Hoerchler
Evaluation Of Ispe For Inhibitor Design In The Non-Mevalonate Pathway, Kataryzna B. Hoerchler
Graduate Research Theses & Dissertations
There is an urgent need to discover new antibiotics to combat the emergence of drug resistant Novel antibiotics that target unique pathways different from existing therapies are promising targets. The non-mevalonate pathway (MEP), which was discovered in the 1990’s, is essential for most bacteria, plants and apicomplexan parasites. Inhibition of the non-mevalonate is an attractive pharmaceutical target, since it is not present in mammals. Many compounds have surfaced as potential inhibitors, but none have made it to market. This thesis investigates the fourth step in the MEP pathway involving the enzyme 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE) from Escherichia coli (Ec), Burkholderia thailandensis …
Quantitation Without Calibration: Response Profile As An Indicator Of Target Amount, Mrittika Debnath
Quantitation Without Calibration: Response Profile As An Indicator Of Target Amount, Mrittika Debnath
Graduate Research Theses & Dissertations
Quantitative assessment of biomarkers is essential in numerous contexts from decision-making in clinical situations to food quality monitoring to the interpretation of life-science research findings. However, appropriate quantitation techniques are not as widely addressed as detection methods. One of the major challenges in biomarker’s quantitation is the need to have a calibration for correlating a measured signal to a target amount. The step complicates the methodologies and makes them less sustainable. In this work, we addressed the issue via a new strategy: relying on the position of response profile rather than absolute signal for assessment of a target nucleic acid’s …