Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Amphiphilic (1)
- Amylin (1)
- Antimicrobial polymers (1)
- Assay development (1)
- Dendrimers (1)
-
- Diabetes (1)
- Disulfide-Shuffling (1)
- HIAPP (1)
- Host defense Peptide (1)
- Immunoglobulin (1)
- Inclusion Body (1)
- LC-MRM (1)
- M-protein (1)
- MPP8 (1)
- Neurotrophin (1)
- Peptidomimetics (1)
- Personalized biomarker (1)
- Protein-protein interaction (1)
- RAS Signaling (1)
- Self-Assembly (1)
- Unimolecular micelles (1)
- Urea (1)
- Γ-AApeptide (1)
Articles 1 - 4 of 4
Full-Text Articles in Chemistry
Interrogation Of Protein Function With Peptidomimetics, Olapeju Bolarinwa
Interrogation Of Protein Function With Peptidomimetics, Olapeju Bolarinwa
USF Tampa Graduate Theses and Dissertations
Proteins can be described as the “machineries” responsible for almost all tasks in the levels of organizational complexity in multi-cellular organisms namely: the cells, tissues, organs and systems. Any disorder in the function of a protein at any of these levels could result in disease, and a study of protein function is critical to understanding the pathological features of the disease at the molecular level. A quick glance at these abundantly present proteins reveals two striking features: large diversity of biological function, and the variations in structural complexity, which varies from simple random coils, to turns and helices, and on …
Quantitative Proteomics To Support Translational Cancer Research, Melissa Hoffman
Quantitative Proteomics To Support Translational Cancer Research, Melissa Hoffman
USF Tampa Graduate Theses and Dissertations
Altered signaling pathways, which are mediated by post-translational modifications and changes in protein expression levels, are key regulators of cancer initiation, progression, and therapeutic escape. Many aspects of cancer progression, including early carcinogenesis and immediate response to drug treatment, are beyond the scope of genetic profiling and non-invasive monitoring techniques. Global protein profiling of cancer cell line models, tumor tissues, and biofluids (e.g. serum or urine) using mass spectrometry-based proteomics produces novel biological insights, which support improved patient outcomes. Recent technological advances resulting in next-generation mass spectrometry instrumentation and improved bioinformatics workflows have led to unprecedented measurement reproducibility as well …
Elastin-Like Polypeptide Fusion Tag As A Protein-Dependent Solubility Enhancer Of Cysteine-Knot Growth Factors, Tamina L. Johnson
Elastin-Like Polypeptide Fusion Tag As A Protein-Dependent Solubility Enhancer Of Cysteine-Knot Growth Factors, Tamina L. Johnson
USF Tampa Graduate Theses and Dissertations
Elastin-like peptide (ELP) fusions promote therapeutic delivery and efficacy. Recombinant proteins, like neurotrophins, lack bioavailability, have short in vivo half-lives, and require high manufacturing costs. Fusing recombinant proteins with genetically encodable ELPs will increase bioavailability, enhance in vivo solubilization, as well as provide a cost-effective method for purification without the need for chromatography. During expression of neurotrophin-ELP (N-ELP) fusions, dense water-insoluble aggregates known as inclusion bodies (IBs) are formed. Inclusion bodies are partially and misfolded proteins that usually require denaturants like Urea for solubilization. Strong denaturants arrest ELPs stimuli-responsive property and increase unwanted aggregation, making purification difficult, yet possible. The …
Design, Synthesis, Application Of Biodegradable Polymers, Mussie Gide
Design, Synthesis, Application Of Biodegradable Polymers, Mussie Gide
USF Tampa Graduate Theses and Dissertations
Bacterial infections have posed a serious threat to the public health due to the significant rise of the infections caused by antibiotic-resistant bacteria. There has been considerable interest in the development of antimicrobial agents which mimic the natural HDPs, and among them biodegradable polymers are newly discovered drug candidates with ease of synthesis and low manufacture cost compared to synthetic host defense peptides. Herein, we present the synthesis of biocompatible and biodegradable polymers including polycarbonate polymers, unimolecular micelle hyperbranched polymers and dendrimers that mimic the antibacterial mechanism of HDPs by compromising bacterial cell membranes. The developed amphiphilic polycarbonates are highly …