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Articles 1 - 4 of 4

Full-Text Articles in Chemistry

Phytosociological Analysis Of Restored And Managed Grassland Habitat Within An Urban National Park, Andrew M. Greller, Celestine Durando, Leslie F. Marcus, D. Siril A. Wijesundara, Michael D. Byer, Robert Cook, John T. Tanacredi Ph.D. Oct 2000

Phytosociological Analysis Of Restored And Managed Grassland Habitat Within An Urban National Park, Andrew M. Greller, Celestine Durando, Leslie F. Marcus, D. Siril A. Wijesundara, Michael D. Byer, Robert Cook, John T. Tanacredi Ph.D.

Faculty Works: CERCOM

Floyd Bennett Field (FBF), 579 ha in extent, is a division of Gateway National Recreation Area. It is the site of a former airfield, constructed by filling salt marshes with dredged materials. Except for the portion known locally as the “North Forty,” all sections of FBF have been cut over to maintain low vegetation. A grassland management plan (GRAMP) for 165 ha was initiated in 1986, to maintain habitats for open-country birds. Over the next few years, encroaching woody vegetation was removed manually and mechanically from the management area. Since then, it has been maintained as a grassland and receives …


Characterization Of Chimeric Lipopolysaccharides From Escherichia Coli Strain Jm109 Transformed With Lipooligosaccharide Synthesis Genes (Lsg) From Haemophilus Influenzae, Nancy J. Phillips, T. J. Miller, J. J. Engstrom, William Melaugh, R. Mclaughlin, M A. Apicella, B W. Gibson Jan 2000

Characterization Of Chimeric Lipopolysaccharides From Escherichia Coli Strain Jm109 Transformed With Lipooligosaccharide Synthesis Genes (Lsg) From Haemophilus Influenzae, Nancy J. Phillips, T. J. Miller, J. J. Engstrom, William Melaugh, R. Mclaughlin, M A. Apicella, B W. Gibson

Chemistry Faculty Publications

Previously, we reported the expression of chimeric lipopolysaccharides (LPS) in Escherichia coli strain JM109 (a K-12 strain) transformed with plasmids containing Haemophilus influenzae lipooligosaccharide synthesis genes (lsg) (Abu Kwaik, Y., McLaughlin, R. E., Apicella, M. A., and Spinola, S. M. (1991) Mol. Microbiol. 5, 2475–2480). In this current study, we have analyzed the O-deacylated LPS and free oligosaccharides from three transformants (designated pGEMLOS-4, pGEMLOS- 5, and pGEMLOS-7) by matrix-assisted laser desorption ionization, electrospray ionization, and tandem mass spectrometry techniques, along with composition and linkage analyses. These data show that the chimeric LPS consist of the complete E. coli LPS core …


Theories Of The Origin And Early Evolution Of Life, David A. Dewitt Jan 2000

Theories Of The Origin And Early Evolution Of Life, David A. Dewitt

Faculty Publications and Presentations

After the theory of spontaneous generation was discredited, only religious explanations were offered to explain the origin of life. Alexander Oparin (1894-1980), an atheist, suggested that natural chemical reactions produced biological molecules that came together to form the first living thing. Later, Stanley Miller tested this hypothesis and produced chemical "building blocks" but not life itself. In spite of much progress, there is still no clear consensus on how life originated on Earth. Some scientists are even looking to outer space for the origin of life.


Cytotoxic Mab From Rheumatic Carditis Recognizes Heart Valves And Laminin, Jeffrey E. Galvin, Mark E. Hemric, Kent Ward, Madeleine W. Cunningham Jan 2000

Cytotoxic Mab From Rheumatic Carditis Recognizes Heart Valves And Laminin, Jeffrey E. Galvin, Mark E. Hemric, Kent Ward, Madeleine W. Cunningham

Faculty Publications and Presentations

Anti-streptococcal antibodies cross-reactive with N-acetyl-bD-glucosamine (GlcNAc) and myosin are present in the sera of patients with rheumatic fever (RF). However, their role in tissue injury is not clear. In this study, we show that anti-GlcNAc/anti-myosin mAb 3.B6 from a rheumatic carditis patient was cytotoxic for human endothelial cell lines and reacted with human valvular endothelium and underlying basement membrane. Reactivity of mAb 3.B6 with the valve was inhibited by human cardiac myosin > laminin > GlcNAc. The mAb 3.B6 epitopes were localized in fragments of human cardiac myosin, including heavy meromyosin (HMM), the S1 subfragment, and two light meromyosin (LMM) peptides containing …