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Full-Text Articles in Chemistry
Selenium Probes For The Detection Of Thioredoxin Reductase Activity, Tendai Joseph Mafireyi
Selenium Probes For The Detection Of Thioredoxin Reductase Activity, Tendai Joseph Mafireyi
Dissertations and Theses
The identification and quantification of proteins and enzymatic activity in living tissues frequently requires the use of invasive procedures such as biopsy. These techniques can inhibit measurements of protein levels in real-time, and the disruption of tissue can lead to the loss of important information concerning the spatial distribution and differential activities in various cell types. The use of small organic fluorescent probes that rely on activation or accumulation within a tissue embody robust methods of detection that address these issues. This dissertation describes the design, synthesis and evaluation of fluorescent probes that can detect the enzyme thioredoxin reductase (TrxR). …
The Combined Effect Of Cu And Zn On Selenastrum Capricornutum, Helmer Colonia-Roque
The Combined Effect Of Cu And Zn On Selenastrum Capricornutum, Helmer Colonia-Roque
Dissertations and Theses
It has been demonstrated that the toxicity of heavy metals depends on their chemical speciation and can be related to their free ion activities, rather than the total metal concentrations. The objective of this study was to determine the effect of combined free ions of zinc and copper on the toxicity to the green alga Selenastrum capricornutum. This alga was grown in a defined medium, under controlled laboratory conditions, with a varying range of zinc and copper concentrations. The growth rate of the alga was inhibited at pZn = 5.93 or at pCu = 7.24. The results suggest that …