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Development Of An Assay To Screen For Novel Proteins From Bacillus Thuringiensis With Antibacterial Activity, Auriole T. Tamegnon
Development Of An Assay To Screen For Novel Proteins From Bacillus Thuringiensis With Antibacterial Activity, Auriole T. Tamegnon
Theses and Dissertations - UTB/UTPA
Bacillus thuringiensis (Bt) produces the non specific Cyt protein and the specific Cry protein. Past research has shown these proteins are toxic to insects, red blood cells, cancer cells, and some bacteria. While many antibacterial substances are known to exist, efforts to find new ones still continue. In this project we used Bt 6-2-56 (containing a novel filament associated parasporal body which appears non-toxic to insects and protozoan) and Bt 4Q13 (which has been shown to have activity against E. coli and S. aureus) to develop a simple, easy, scalable assay in a 96 well Microtitre plate to look for …