Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- 16S rDNA gene (1)
- Aptamer (1)
- Electrochemical aptamer-based sensor (1)
- Exonuclease (1)
- Forensic Identification (1)
-
- Gas chromatography mass spectrometry (GCMS) (1)
- Headspace Solid phase microextraction (HS-SPME) (1)
- Human Odor (1)
- In vitro Selection (1)
- Microbiome (1)
- Multianalyte detection (1)
- Next generation sequencing (NGS) (1)
- SELEX (1)
- Sensing (1)
- Small molecule detection (1)
- Small molecules (1)
- Volatile Organic Compounds (VOCs) (1)
Articles 1 - 3 of 3
Full-Text Articles in Chemistry
Development Of Electrochemical Aptamer-Based Sensors For Sensitive And Specific Detection Of Small-Molecule Targets, Yingzhu Liu
Development Of Electrochemical Aptamer-Based Sensors For Sensitive And Specific Detection Of Small-Molecule Targets, Yingzhu Liu
FIU Electronic Theses and Dissertations
Electrochemical aptamer-based (E-AB) sensors have been applied for diverse applications such as forensic science, pharmaceutical diagnostics, food safety, environmental monitoring, and personal medical care due to their rapid, accurate, and specific detection of analytes in complex samples. However, limited sensitivity and a lack of portable devices for point-of-care applications have greatly hindered the maturation of E-AB sensors from proof-of-concept designs to commercial systems. This dissertation describes several novel strategies to improve the sensitivities of E-AB sensors and fabricate portable paper-based devices to remedy these problems. We first detail the development of a novel approach to utilize a defined mixture of …
Exonuclease Digestion Assay For Streamlining Aptamer Characterization, Engineering, And Sensor Development, Juan Canoura
Exonuclease Digestion Assay For Streamlining Aptamer Characterization, Engineering, And Sensor Development, Juan Canoura
FIU Electronic Theses and Dissertations
Aptamers are DNA or RNA oligonucleotide-based bioreceptors isolated in vitro through the Systematic Evolution of Ligands by Exponential Enrichment. Given the ease with which a selection can be customized, aptamers can be evolved to function in nearly any chemical environment, making them tailormade for their final application. However, the post-SELEX characterization of the 100-1000’s aptamer candidates remains a significant bottleneck as there are no suitable techniques for high-throughput characterization of each candidate’s affinity/specificity. Moreover, the final aptamer must be engineered to possess signal reporting functionality; this is often done via trail-and-error truncation to yield a structure-switching aptamer. This dissertation describes …
An Investigation To Corroborate Volatile And Biological Profiles Of Human Odor For Forensic Subject Identification, Chantrell J.G. Frazier
An Investigation To Corroborate Volatile And Biological Profiles Of Human Odor For Forensic Subject Identification, Chantrell J.G. Frazier
FIU Electronic Theses and Dissertations
Hands are an integral part in transferring complex microbial communities to and from our surroundings. As a result, hands are significant in provenance investigations as specific microbiota can be deposited on everyday objects through touch interactions. Skin microbiome, including bacteria, fungi, and viruses, are unique to each person, and this ‘uniqueness’ can be exploited and applied to forensic identification. Skin microbiota and volatile organic compounds (VOCs) are closely related due to specific bacteria breaking down non-volatile organic compounds to volatile organic compounds that are characteristically present in human scent. However, analyses of microbiota from touch interactions have proven to be …