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Development Of Amperometric Enzyme Electrodes For Glucose And Hypoxanthine., Eilish Broderick
Development Of Amperometric Enzyme Electrodes For Glucose And Hypoxanthine., Eilish Broderick
Theses
This thesis is concerned with the immobilisation of enzymes on electrosynthesised poly (o- phenylenediamine) membranes for use on amperometric electrodes. The thesis opens with a discussion of the basic principles of enzymology and a review of a diverse range of biosensors, followed by a consideration of amperometry as governed by the Cottrell equation.
Glucose oxidase and horseradish peroxidase were co-immobilised in poly (o-phenylenediamine) on platinum electrodes at + 0.6 V versus Ag/AgCl. Using this approach the enzymes are entrapped in a strongly adherent, highly reproducible thin membrane, of thickness approximately 10 nm. This one-step procedure produces a glucose sensor with …