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Full-Text Articles in Chemistry
Hiv-1 Rna Dimerization At Single Molecule Level, Hansini R. Mundigala
Hiv-1 Rna Dimerization At Single Molecule Level, Hansini R. Mundigala
Wayne State University Dissertations
The Dimerization Initiation Sequence (DIS) is a conserved hairpin-loop motif on the 5' UTR of the HIV-1 genome. It plays an important role in genome dimerization through formation of a "kissing complex" intermediate between two homologous DIS sequences. This bimolecular kissing complex ultimately leads to the formation of an extended RNA duplex. Understanding the kinetics of this interaction is key to exploiting DIS as a possible drug target against HIV. We wish to report a novel study that makes an important contribution to understanding the dimerization mechanism of HIV-1 RNA in vitro. Our work has employed single-molecule fluorescence resonance energy …
New Carbohydrate-Based Anti-Cancer And Anti-Bacterial Vaccines, Zhifang Zhou
New Carbohydrate-Based Anti-Cancer And Anti-Bacterial Vaccines, Zhifang Zhou
Wayne State University Dissertations
The unique carbohydrates expressed on the surface of cancer, bacterial, viral and fungal cells are excellent target antigens for the design of therapeutic or preventive vaccines. However, as antigens carbohydrates have problems. First, carbohydrates usually have low immunogenicity. Second, even if immunogenic, carbohydrates typically elicit T cell-independent immune responses. To overcome these problems and design useful vaccines based on carbohydrate antigens, they are usually coupled with carrier proteins to form conjugates to enhance the immunogenicity of the antigens. However, there are still some issues existing in glycoprotein vaccines, such as poor reproducibility of the conjugates, difficulties in quality control and …