Chemistry Commons^™

Novel Applications Of Mass Spectrometry For Quantitation And Reaction Mechanism Elucidation, Pengyi Zhao

Dissertations

Mass spectrometry (MS) has been growing as one of the most widely used tools in the field of analytical chemistry. Various applications have been developed to harness the high sensitivity and specificity of mass spectrometric analysis. In this dissertation, two major challenges are addressed. By developing mass spectrometric-based methods, absolute quantitation of proteins/peptides have been achieved. Elucidation of various reaction mechanisms are also enabled. These are the focuses of this dissertation.

In Chapters 2 to 4, a novel quantitation method is developed, titled as coulometric mass spectrometry (CMS). The strength of this method is that no reference standard or isotope-labeled …

Metal Ions Impact On Shewanella Oneidensis Mr-1 Adhesion To Ito Electrode And The Enhancement Of Current Output, Aisha Awad Alshahrani

Dissertations

The goal of this study is to enhance the efficiency of bacterial extracellular electron transfer (EET) in Shewanella oneidensis MR-1 by enhancing adhesion to the electrode's surface. Our results clearly show a major difference in the attachment and behavior of Shewanella oneidensis MR-1 for Ca2+, Pb2+, Cd2+, and Mg2+, compared to the control. the final microbial coverage, as measured by confocal microscopy and cathodic peak charge in cyclic voltammetry (Qpc), increases with increasing metal ion concentrations. We found the cells attached to the electrode increased more with the addition of metal ion concentrations in the following order of metals: Ca2+ …

Development Of Methods For The Removal Of Selected Pollutants From Several Matrices And Identification Of Unknown Pollutants Adsorbed Onto Plastics Collected From Freshwater, Kathryn Marie Renyer

Dissertations

Plastic pollution represents one of greatest anthropogenic threats to the environment. Five to ten billion tons of plastic are manufactured every year. Currently, Earth's ecosystem is contaminated with billions of tons of plastic debris, much of which cannot be recycled. Over time, this plastic debris decomposes into small particles. Small plastic particles are known to adsorb toxic compounds in marine environments. My research is concerned with creating novel methods for the detection and quantification of selected persistent organic pollutants from several media. Specifically, I developed methods for the detection and quantification of endosulfan sulfate (ESS) from Lumbricus terrestris tissue and …

Electron Transfer Of Shewanella Oneidensis Mr-1 At Clay-Modified Ito Electrode, Reem Faez Alshehri

Dissertations

Various strategies have been established to enhance the extracellular electron transfer and energy output capability of microbial fuel cells, with the majority being aimed at anode modification. The anode has a significant impact on the electricity generation performance of MFCs because it is in direct contact with the microorganisms. The materials of the anode should be favorable for the bacterial cell and capable to facilitate the electron transfer. Developing of an electrode using low-cost and effective materials assists to enhance the bacterial cell attachment and extracellular electron transfer. This provides a significant improvement in MFC performance. In this study, clay …