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Genetic engineering

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Full-Text Articles in Physical Sciences and Mathematics

Embok 5.0 - Industry 4.0/5.0 Manifest And Latent Dimensions Mapping To The Asem Embok, T. Steven Cotter Jan 2023

Embok 5.0 - Industry 4.0/5.0 Manifest And Latent Dimensions Mapping To The Asem Embok, T. Steven Cotter

Engineering Management & Systems Engineering Faculty Publications

Industry 3.0 automation emerged replacing human labor with high volume processes and robotics. Industry 4.0, cyber-physical systems, and Industry 5.0, mass customization and cognitive systems, are in the early stages of emergence. Research into the impact of Industry 4.0 and 5.0 is focused at the strategic or organizational levels or on the technological challenges. Research into the impact of Industry 4.0 and 5.0 on engineering management has been limited to their impact on project management. This leaves open the question of the directions in which ASEM should evolve the Engineering Management Body of Knowledge (EMBOK) under the emergence of Industry …


Improvement Of Biochar Through Ozonization And Biosafety Of Genetically Engineered Cyanobacteria, Oumar Sacko Jul 2021

Improvement Of Biochar Through Ozonization And Biosafety Of Genetically Engineered Cyanobacteria, Oumar Sacko

Chemistry & Biochemistry Theses & Dissertations

Through the innovative technique of biochar post-production surface oxygenation by ozonization, we were able to improve certain properties of biochar. In project one, the incubation of an insoluble phosphate rock material (hydroxyapatite) with the wet ozonized pine 400 biochar and its filtrate resulted in a solubilization of 80 times more phosphate from hydroxyapatite (569.0 mg/L ± 6.4) compared to the pure water-hydroxyapatite control (7.2 mg/L ± 0.3). The ozonized biochar may provide a new possible way to unlock the phosphorus from insoluble phosphate mineral phases. The cation exchange capacity (CEC) is a key property of biochar when used as a …


Algal Biofuel: The Future Of Green Jet Fuel In Air Transportation, Rajee Olaganathan Apr 2018

Algal Biofuel: The Future Of Green Jet Fuel In Air Transportation, Rajee Olaganathan

Publications

The aviation industry is one of the major contributors for the greenhouse gases. As air travel has become inevitable in this modern era, and fossil fuel usage is not sustainable, it is essential to produce renewable fuel and commercialize it to reduce the greenhouse gas emissions. In order to produce an alternate aviation biofuel a lot of industrial and academic collaborations have been developed worldwide. The main concern of this collaborative research is to produce aviation biofuel from renewable resources with low environmental impacts, and which is sustainable at an economically viable price. This mini-review briefly discusses the biotechnological approaches …


Genetically Engineered Polypeptides As Biomimetic Light Harvesting Optical Antenna, Jason P. Seeley Jan 2018

Genetically Engineered Polypeptides As Biomimetic Light Harvesting Optical Antenna, Jason P. Seeley

Legacy Theses & Dissertations (2009 - 2024)

Natural resources useful for the generation of energy are limited. The development of efficient materials capable of utilizing the abundant free solar radiation is of considerable interest. Utilization of otherwise wasted energy sources, including solar radiation, is a progressive step in the quest for sustainable energy. Solar radiation incident upon the earth’s surface exceeds current energy requirements and motivates scientists to investigate and develop functional devices and nanomaterials including light harvesting complexes (LHC) capable of capturing solar radiation for energy conversion and storage.


So, You Want To Use Next Generation Sequencing In Marine Systems? Insight From The Pan Pacific Advanced Studies Institute, D. A. Willette, F. W. Allendorf, P. H. Barber, D. J. Barshis, K. E. Carpenter, E. D. Crandall, W. A. Cresko, I. Fernandez-Silva, M. V. Matz, E. Meyer, M. D. Santos, L. W. Seeb, J. E. Seeb Jan 2014

So, You Want To Use Next Generation Sequencing In Marine Systems? Insight From The Pan Pacific Advanced Studies Institute, D. A. Willette, F. W. Allendorf, P. H. Barber, D. J. Barshis, K. E. Carpenter, E. D. Crandall, W. A. Cresko, I. Fernandez-Silva, M. V. Matz, E. Meyer, M. D. Santos, L. W. Seeb, J. E. Seeb

Biological Sciences Faculty Publications

The emerging field of next-generation sequencing (NGS) is rapidly expanding capabilities for cutting edge genomic research, with applications that can help meet marine conservation challenges of food security, biodiversity loss, and climate change. Navigating the use of these tools, however, is complex at best. Furthermore, applications of marine genomic questions are limited in developing nations where both marine biodiversity and threats to marine biodiversity are most concentrated. This is particularly true in Southeast Asia. The first Pan-Pacific Advanced Studies Institute (PacASI) entitled "Genomic Applications to Marine Science and Resource Management in Southeast Asia" was held in July 2012 in Dumaguete, …


Genetically Engineered Repetitive Polypeptides : Design, Synthesis, Characterization, And Applications, Natalya I. Topilina Jan 2009

Genetically Engineered Repetitive Polypeptides : Design, Synthesis, Characterization, And Applications, Natalya I. Topilina

Legacy Theses & Dissertations (2009 - 2024)

This work is dedicated to the investigation of protein folding with the emphasis on the aggregation and amyloid fibrils formation. The focus of the research is to elucidate the relationship between polypeptide chain sequence and folding/aggregation properties. For this purpose, i) libraries of the de novo macromolecular repetitive β-sheet forming polypeptides were designed, and ii) the strategy for their rapid creation was developed. The strategy enables retention of a basic polypeptide core, yet allowing variations in the repetitive sequence, degree of polymerization and distribution of specific amino acids. The polypeptides were expressed in E. coli, purified, and characterized by various …


Optimization Of A Ribosomal Structural Domain By Natural Selection, Corina Maeder, G. L. Conn, D. E. Draper May 2006

Optimization Of A Ribosomal Structural Domain By Natural Selection, Corina Maeder, G. L. Conn, D. E. Draper

Chemistry Faculty Research

A conserved, independently folding domain in the large ribosomal subunit consists of 58 nt of rRNA and a single protein, L11. The tertiary structure of an rRNA fragment carrying the Escherichia coli sequence is marginally stable in vitro but can be substantially stabilized by mutations found in other organisms. To distinguish between possible reasons why natural selection has not evolved a more stable rRNA structure in E. coli, mutations affecting the rRNA tertiary structure were assessed for their in vitro effects on rRNA stability and L11 affinity (in the context of an rRNA fragment) or in vivo effects on cell …


Mirrorless All‐Optical Bistability In Bacteriorhodopsin, D.V.G.L.N. Rao, F. J. Aranda, B. J. Wiley, J. A. Akkar, D. L. Kaplan, J. F. Roach Sep 1993

Mirrorless All‐Optical Bistability In Bacteriorhodopsin, D.V.G.L.N. Rao, F. J. Aranda, B. J. Wiley, J. A. Akkar, D. L. Kaplan, J. F. Roach

Physics Faculty Publications

We report direct observations of all‐optical mirrorless bistability associated with saturable absorption in three kinds of bacteriorhodopsin (BR) samples: wild‐type BR in water solution and dispersed in thin films of a polymer matrix as well as water solution of the genetically engineered mutant BRD96N. The experiments are carried out with picosecond pulses at 532 nm. The values measured for the saturation intensity are explained in terms of the relaxation of the excited M state population to the B state of the BR photocycle for the three kinds of samples studied.


Biological Pesticides: Biotechnology's Answer To Silent Spring, Donald H. Dean Jan 1989

Biological Pesticides: Biotechnology's Answer To Silent Spring, Donald H. Dean

Journal of the Iowa Academy of Science: JIAS

In the 25 years since the publication of Rachel Carson’s Silent Spring the public has come to realize the environmental impact of heavy use of chemical pesticides. To add insult to injury, many insects, including the disease vector, the mosquito, are now virtually resistant to standard chemical pesticides. Biotechnology is now providing a positive response to these dilemmas through the production and development of improved forms of microbial pest control agents: biological pesticides. Biological pesticides are pathogens, or predators, of insects such as bacteria, fungi, and viruses, and predatory insects or vertebrates such as mosquito fish which reduce the population …


Public Policy On The Introduction Of Genetically Engineered Microorganisms, Anne K. Vidaver Jan 1989

Public Policy On The Introduction Of Genetically Engineered Microorganisms, Anne K. Vidaver

Journal of the Iowa Academy of Science: JIAS

This presentation raises questions of research needs and issues. Underlying assumptions are that only beneficial or useful microorganisms will be "released"; that extensive laboratory and contained experiments will have been done prior to introduction and live microorganisms can be confined within the areas of introduction. Evidence to support these assertions will be presented. Critical needs for progress in this area include: 1) Recognition that the nature of the product introduced into the environment is of primary significance, not how the organism was genetically altered or modified. 2) Recognition that microorganisms are introduced into the environment as part of our daily …


Release And Behavior Of Recombinant Bacteria In Field Studies, Steven E. Lindow Jan 1989

Release And Behavior Of Recombinant Bacteria In Field Studies, Steven E. Lindow

Journal of the Iowa Academy of Science: JIAS

Numerous opportunities exist for the utilization of genetically engineered microorganisms for useful purposes in agriculture and in waste management. An extremely diverse array of microorganisms is likely to be considered for such processes as biological control of plant pests, degradation of to toxic wastes, reclamation of rare metals and other processes. For some purposes, such as in the degradation of toxic materials, it may be possible to make biologically compromised microorganisms that will exist only in the presence of the toxic chemicals that they were designed to transform. A better understanding of the genetics, biology and physiology of microorgansims which …