Physical Sciences and Mathematics Commons^™

The Impacts Of Anthropogenic Activity And Climate Change On The Formation Of Harmful Algal Blooms (Habs) And Its Ecological Consequence, Zhangxi Hu, Aifeng Li, Zhun Li, Margaret R. Mulholland

OES Faculty Publications

No abstract provided.

Using Diatoms To Reconstruct Eutrophication In Lake Carmi, Vt, Margaret Polifrone, Sarah Wasserman, Ismar Biberovic, Kaleb Jones, Andrew Schroth, Andrea Lini, Ana Morales-Williams

Lake Champlain Sea Grant Institute

No abstract provided.

Segmentation Of Diatoms Using Edge Detection And Deep Learning, Hüseyi̇n Gündüz, Cüneyd Nadi̇r Solak, Serkan Günal

Turkish Journal of Electrical Engineering and Computer Sciences

Diatoms are photosynthesizing algae found in almost every aquatic environment. Detecting the number and diversity of diatoms is very important to analyze water quality appropriately. Accurate segmentation of diatoms is therefore crucial for this detection process. In this study, a new and effective model for the automatic segmentation of diatoms based on image processing and deep learning algorithms is proposed. In the proposed model, edge segments of a given image containing diatoms and nondiatom particles are first obtained. These edge segments are then combined, resulting in closed contours representing diatom candidates. In the final step, the diatom candidates are classified …

Diatom Response To Different Hydrologic Sources In Alpine Streams: A Teton Range Case Study, Shannon Weld

Water Resources Professional Project Reports

Alpine stream diversity is due in part to different hydrological sources including snowpack, surface glaciers, and rock glaciers; climate change threatens to homogenize these sources. Surprisingly little is understood about algal communities in these stream types. We characterized algal communities and water chemistry among ten alpine streams from these sources in the Teton Range, Wyoming, USA. Late summer sampling (2019-2021) included diatoms, anions, cations, and water quality parameters. Data were analyzed using one- and two-way analysis of variance, principal component analysis, and nonmetric multidimensional scaling. There were statistically significant differences among some or all hydrologic sources for temperature, dissolved oxygen, …

Biostratigraphy Of Paleogene Diatom Assemblages In The Southern Ocean, Angela Kaup

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

The record of siliceous microfossil sedimentation in the high latitude South Atlantic Ocean has great potential for dating seismic and stratigraphic units. Over the last several decades, scientists have documented diatom biostratigraphic record from sediment cores and drill cores in the Falkland Plateau and Maurice Ewing Bank region, as well as other areas of the Southern Ocean, and a robust chronostratigraphic framework is available for Neogene sequences. Given the complicated nature of ocean bathymetry, tectonic plate motion (vertical and lateral), and ocean current flow, the sedimentological evolution of this oceanic region is not well understood. Sampling sediment cores at high …

Drivers Of Extracellular Polysaccharide Production By A Mat-Forming Diatom, Kaitlin A. Stansbury

FIU Electronic Theses and Dissertations

Microbial biofilms are held together by extracellular polymeric substances (EPS), which can be secreted by many organisms. EPS production can facilitate intercellular communication and inter-guild microbial mutualisms, intraspecific gamete exchange, nutrient sequestration, and desiccation resistance. Benthic microbial mats (periphyton) of the Florida Everglades and other karstic wetlands contain abundant EPS generated by mat-producing filamentous blue-green algae and many other species of mat-dwelling algae, cyanobacteria, and bacteria, including the most abundant Everglades diatom, Mastagloia calcarea. The benthic diatom genus Mastogloia is characterized by several morphological and physiological features that foster production of a ‘halo’ of EPS around the frustule, but the …

Miocene-Pleistocene Diatom Oxygen Isotope And Biogenic Silica Record From Iodp Site U1523, Ross Sea, Antarctica, Donald James Mclennan

Graduate Research Theses & Dissertations

The West Antarctic Ice Sheet (WAIS) plays a significant role in Earth’s climate. WAIS is currently retreating due to changes in wind-driven oceanic currents resulting in an increasing flux of warm waters to ice margins. Brine rejection in the Ross Sea is also major source of High Salinity Shelf water (HSSW) and Antarctica Bottom Waters (AABW), which creates a stratified ocean and plays a significant role in regulating Earth’s climate. Currently, there are very limited proxies that can quantify the variable input of HSSW formation on geologic time scales. Here we provide a 18Odiatom and biogenic silica (BSi) record which …

Aggregation And Degradation Of Dispersants And Oil By Microbial Exopolymers (Addomex): Toward A Synthesis Of Processes And Pathways Of Marine Oil Snow Formation In Determining The Fate Of Hydrocarbons, Antonietta Quigg, Peter H. Santschi, Chen Xu, Kai Ziervogel, Manoj Kamalanathan, Wei-Chun Chin, Adrian B. Burd, Andrew Wozniak, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

Microbes (bacteria, phytoplankton) in the ocean are responsible for the copious production of exopolymeric substances (EPS) that include transparent exopolymeric particles. These materials act as a matrix to form marine snow. After the Deepwater Horizon oil spill, marine oil snow (MOS) formed in massive quantities and influenced the fate and transport of oil in the ocean. The processes and pathways of MOS formation require further elucidation to be better understood, in particular we need to better understand how dispersants affect aggregation and degradation of oil. Toward that end, recent work has characterized EPS as a function of microbial community and …

Diatom Community Composition Shifts Driven By Coherent Cyclonic Mesoscale Eddies In The California Current System, Zuzanna Maria Abdala

OES Theses and Dissertations

The California Current System (CCS) is characterized by an equatorward flowing eastern boundary current, as well as seasonal wind-driven coastal upwelling which supplies nutrient-rich waters to the surface and drives high coastal productivity. Cyclonic mesoscale eddies form off the coast in the CCS where they trap the highly productive upwelled coastal waters, along with their resident planktonic communities, and transport them offshore into the more oligotrophic California Current waters. The interaction between waters within and outside of the eddies is limited, and so the eddies act as natural mesocosms, where the resident phytoplankton population undergo ecological succession as the eddy …

Traces Of Mediterranean Origin Holocene Transgression In The Drainage Basin Of Riva-Çayağzı Creek Of The Black Sea (Northeastern İstanbul-Turkey), Bülent Doğan, Ahmet Karakaş, Ati̇ke Nazi̇k, İbrahi̇m Engi̇n Meri̇ç

Turkish Journal of Earth Sciences

The Riva-Çayağzı Creek (İstanbul, Black Sea) pours into the Black Sea from the coasts of Turkey. In the drainage basin of this creek, both deep and shallow drillings were undertaken, and the minimum thickness of Holocene stratigraphy was determined as 30 m. The Holocene young deposits are mainly composed of fine-grained sediments and clay, which has the most prominent thickness. The energy of water during the sedimentation process was low and stagnant in the Riva-Çayağzı Creek basin. When the marine mollusc fossils obtained from the shallow boring cores are evaluated, it can be deduced that the sea level in the …

The Impact Of Eddies On Nutrient Supply, Diatom Biomass And Carbon Export In The Northern South China Sea, Yung-Yen Shih, Chin-Chang Hung, Sing-How Tuo, Huan-Jie Shao, Chun Hoe Chow, Francois L.L. Muller, Yuan-Hong Cai

OES Faculty Publications

We have investigated the effect of eddies (cold and warm eddies, CEs and WEs) on the nutrient supply to the euphotic zone and the organic carbon export from the euphotic zone to deeper parts of the water column in the northern South China Sea. Besides basic hydrographic and biogeochemical parameters, the flux of particulate organic carbon (POC), a critical index of the strength of the oceanic biological pump, was also measured at several locations within two CEs and one WE using floating sediment traps deployed below the euphotic zone. The POC flux associated with the CEs (85 ± 55 mg-C …

Phytoplankton Plastid Proteomics: Cracking Open Diatoms To Understand Plastid Biochemistry Under Iron Limitation, Skyler J. Nunn, Phoebe Dreux Chappell, Kristofer Gomes, Anasthasia Bonderenko, Bethany D. Jenkins, Brook L. Nunn

OES Faculty Publications

Diatoms, such as Thalassiosira pseudonana, are important oceanic primary producers, as they sequester carbon dioxide (CO₂) out of the atmosphere, die, and precipitate to the ocean floor. In many areas of the world’s oceans, phytoplankton, such as diatoms, are limited in growth by the availability of iron (Fe). Fe is an essential nutrient for phytoplankton, as it is central in the electron transport chain component of photosynthesis. Through this study, we examined if Fe-limitation makes a significant difference in the proteins expressed within the chloroplast, the power source for diatoms. Here, we utilized a new plastid isolation technique specific …

Protein Recycling In Bering Sea Algal Incubations, Eli K. Moore, H. Rodger Harvey, Jessica F. Faux, David R. Goodlett, Brook L. Nunn

OES Faculty Publications

Protein present in phytoplankton represents a large fraction of the organic nitrogen and carbon transported from its synthesis in surface waters to marine sediments. Yet relatively little is known about the longevity of identifiable protein in situ, or the potential modifications to proteins that occur during bloom termination, protein recycling and degradation. To address this knowledge gap, diatom-dominated phytoplankton was collected during the Bering Sea spring blooms of 2009 and 2010, and incubated under darkness in separate shipboard degradation experiments spanning 11 and 53 d, respectively. In each experiment, the protein distribution was monited over time using shotgun proteomics, …

Watershed And Aquatic Ecosystem Evolution During The Late-Glacial And Early-Holocene Inferred From High-Resolution Diatom And Geochemical Records In The Yellowstone Region, Yanbin Lu

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

High-resolution diatom records spanning the late-glacial and early-Holocene were developed from three lakes in the Yellowstone region to infer the lake history and to evaluate the relative importance of climatic versus non-climatic factors on the aquatic ecosystem evolution. Synchronous shifts in diatom community structure occurred in all lakes, which are located in regions of varied surficial geology and precipitation seasonality, in the intervals of 13.9-13.8, 13.2-13.1, 11.3-11.0 and 8.8-8.7 cal ka. The synchronous diatom community structure shifts suggest that large-scale climatic change is the primary driver of the aquatic ecosystem evolution in this region, whereas the influence of non-climatic factors …

The Influence Of Basin Morphometry On The Regional Coherence Of Patterns Of Diatom-Inferred Salinity In Lakes Of The Northern Great Plains (Usa), Courtney R. Wigdahl, Jasmine E. Saros, Sherilyn C. Fritz, Jeffery R. Stone, Daniel R. Engstrom

Department of Earth and Atmospheric Sciences: Faculty Publications

Sedimentary diatom profiles from saline lakes are frequently used to reconstruct lakewater salinity as an indicator of drought. However, diatom-inferred salinity (DI-salinity) reconstructions from geographically proximal sites in the Great Plains (USA) have yielded disparate results. This study explores how physical changes in lake habitat resulting from drought may affect climate inferences from salinity reconstructions. Differences in relationships among drought, lake-level change, and diatom community structure over the last century were examined for three saline lakes in the northern Great Plains with dissimilar DI-salinity records. At each site, models were developed relating available planktic:benthic (P:B) habitat area to lake-level change, …

Diatom Community Response To Climate Variability Over The Past 37,000 Years In The Sub-Tropics Of The Southern Hemisphere, Sarah Hembrow, Kathryn Taffs, Pia Atahan, Jeffrey Parr, Atun Zawadzki, Henk Heijnis

Jeffrey Parr

Climate change is impacting global surface water resources, increasing the need for a deeper understanding of the interaction between climate and biological diversity. This is particularly the case in the Southern Hemisphere sub-tropics, where little information exists on the aquatic biota response to climate variations. Palaeolimnological techniques, in particular the use of diatoms, are well established and can significantly contribute to the understanding of climatic variability and the impacts that change in climate have on aquatic ecosystems. A sediment core from Lake McKenzie, Fraser Island (Australia), was used to investigate interactions between climate influences and aquatic ecosystems. This study utilises …

Dynamic Diatom Response To Changing Climate 0-1.2 Ma At Lake El'gygytgyn, Far East Russian Arctic, Jeffrey Allan Snyder, M. V. Cheraponova, A. Bryan

School of Earth, Environment and Society Faculty Publications

The Lake El' gygytgyn sediment record contains an abundant diatom flora through most intervals of the lake's history, providing a means to create and test hypotheses concerning the lake's response to changing climates. The 0 1.2 Ma core interval is characterized by shifts in the dominant planktonic genera and events of exceptional concentration and diversity. Warm interglacial intervals have enhanced concentration and diversity of the plankton. This response is most extreme during exceptional events corresponding to marine isotope stages (MIS) 11 and 31. Diatom concentration and diversity also increase during some cold intervals (e.g., MIS 2), suggesting conditions of lake …

Distributions Of Particulate Heme B In The Atlantic And Southern Oceans- Implications For Electron Transport In Phytoplankton, Martha Gledhill, Eric P. Achterberg, David J. Honey, Maria C. Nielsdottir, Micha J.A. Rijkenberg

OES Faculty Publications

Concentrations of heme b, the iron-containing component of b-type hemoproteins, ranged from b concentrations were enhanced in the photic zone and decreased with depth. Heme b concentrations correlated positively with chlorophyll a (chl a) in the TNA (r=0.41, pb did not correlate with chl a in the IB or SS. In the IB and SS, stations with high-chlorophyll and low-nutrient (Fe and/or Si) concentrations exhibited low heme bconcentrations relative to particulate organic carbon (< 0.1 μmolmol^-1, and high chl a:heme b ratios (> 500). High chl a:heme b ratios resulted from relative decreases in …

Effects Of Deepwater Horizon Oil On The Growth Rates And Pigment Composition Of Phytoplankton Isolated From Grand Isle, La, Jie Li

LSU Master's Theses

This research focused on the effects of un-weathered Macondo crude oil on the growth rates and pigment ratios of phytoplankton isolated from Grand Isle, LA. The experiments involved incubating nutrient-enriched artificial media containing a range of oil concentrations up to 19.2 ppm with small aliquots of coastal water from Grand Isle and measuring the growth rates of the phytoplankton during the subsequent 10–14 days and the pigment ratios of the phytoplankton at the end of log-phase growth to determine whether the crude oil affected the growth rate of the phytoplankton and their composition in terms of pigment ratios. Pigment analysis …

A Diatom Record Of Late Pliocene Cooling From The Ross Sea Continental Shelf, And-1b, Antarctica, Charlotte Sjunneskog, Diane Winter

ANDRILL Research and Publications

A late Pliocene – early Pleistocene, 2.9–2.0Ma, diatom record from the Antarctic Geological Drilling Program (ANDRILL) MIS drillcore AND-1B is presented. This core, recovered from beneath the Ross Ice Shelf south of Ross Island, comprises multiple diatomaceous-sediment units deposited during interglacial periods with open water over the core site. These represent interglacial phases of orbitally paced climate cycles and are punctuated by glacial advances. Extant diatom assemblages have limited presence in the late Pliocene record, which makes environmental interpretation less straight forward. We employ modern ecological data in combination with late Pliocene to present variation in diatom assemblages across the …

Occurrence, Toxicity, And Diversity Of Pseudo-Nitzschia In Florida Coastal Waters, Sheila O'Dea

USF Tampa Graduate Theses and Dissertations

Domoic acid (DA), a potent neurotoxin that has the potential to cause amnesic shellfish poisoning (ASP), is produced by members of the marine diatom genus Pseudo-nitzschia. Outbreaks of ASP in humans and of DA poisoning in birds and marine mammals have been reported across the United States and Canada since the late 1980's. Pseudo-nitzschia species can be extremely abundant in Florida waters, with densities often exceeding 10⁶ cells/L, and sometimes exceeding 10⁷ cells/L. Based on preliminary data, it is evident that at least nine species of Pseudo-nitzschia are found in Florida coastal waters. At least six of …

Environmental History Of A Closed-Basin Lake In The Us Great Plains: Diatom Response To Variations In Groundwater Flow Regimes Over The Last 8500 Cal. Yr Bp, William O. Hobbs, Sherilyn C. Fritz, Jeffery R. Stone, Joseph J. Donovan, Eric C. Grimm, James E. Almendinger

Department of Earth and Atmospheric Sciences: Faculty Publications

Sediment records from closed-basin lakes in the Northern Great Plains (NGP) of North America have contributed significantly to our understanding of regional paleoclimatology. A high-resolution (near decadal) fossil diatom record from Kettle Lake, ND, USA that spans the last 8500 cal. yr BP is interpreted in concert with percent abundance of aragonite in the sediment as an independent proxy of groundwater flow to the lake (and thus lake water level). Kettle Lake has been relatively fresh for the majority of the Holocene, likely because of the coarse substrata and a strong connection to the underlying aquifer. Interpretation of diatom assemblages …

Ip25: A Molecular Proxy Of Sea-Ice Duration In The Bering And Chukchi Seas, Cecily J. Sharko

Masters Theses 1911 - February 2014

Seasonal sea ice is an important component of the global climate system. Sea ice influences exchange rates of heat, moisture, and gas between the ocean and atmosphere. Sea ice also plays critical roles in high latitude ecosystems and marine carbon cycling. Records of sea-ice extent and duration in the Arctic Ocean and its marginal seas through geologic time are valuable resources for better understanding the intricate relationships between sea ice and climate.

IP25, a compound biosynthesized exclusively by diatoms associated with sea ice, has been used to construct qualitative records of sea ice from sediment cores in some areas of …

An Extraordinary Example Of Photokarren In A Sandstone Cave, Cueva Charles Brewer, Chimantá Plateau, Venezuela: Biogeomorphology On A Small Scale, Joyce Lundberg, Donald A. Mcfarlane, Charles Brewer-Carias

WM Keck Science Faculty Papers

A distinctive suite of small-scale erosional forms that are oriented towards the light occur close to the entrance of Cueva Charles Brewer, a large cave in a sandstone tepui, in SE Venezuela. These are the third example of photokarren ever studied in the world, the other two being from Borneo and Ireland. They are the only photokarren ever described from sandstone, and the only example from a non-carbonate environment. The host rock is a poorly-lithified unit of the Precambrian quartz arenite of the Roraima Supergroup. The forms are all oriented towards the light at 30° regardless of rock surface orientation. …

The Path To Preservation: Using Proteomics To Decipher The Fate Of Diatom Proteins During Microbial Degradation, Brook L. Nunn, Ying S. Ting, Lars Malmström, Yihsuan S. Tsai, Angela Aquier, David R. Goodlett, H. Rodger Harvey

OES Faculty Publications

We drew upon recent advances in tandem mass spectrometry-based proteomic analyses in order to examine the proteins that remain after a diatom bloom enters the stationary phase, precipitates out of the photic zone, and is subjected to microbial degradation over a 23-d period within a controlled laboratory environment. Proteins were identified from tandem mass spectra searched against three different protein databases in order to track proteins from Thalassiosira pseudonana and any potential bacterial contributions. A rapid loss of diatom protein was observed over the incubation period; 75% of the proteins initially identified were not detected after 72 h of exposure …

A Microwave Digestion Technique For The Extraction Of Fossil Diatoms From Coastal Lake And Swamp Sediments, Jeffrey Parr, Kathryn Taffs, Christopher Lane

Jeffrey Parr

This study provides an introduction to a microwave digestion technique for the extraction of fossil diatoms from sediments. The microwave technique is compared with the standard diatom extraction technique of Battarbee (Diatom analysis. In: Berglund B.E. (ed.), Handbook of Holocene Palaeoecology and Palaeohydrology. John Wiley and Sons) that uses a combination of dilute hydrochloric acid and hydrogen peroxide and the advantages and disadvantages of their use are discussed. The results suggest that the microwave technique is fast, inexpensive and most importantly produces replicable fossil diatom assemblage data. Small samples sizes are used (0.3 g) for the microwave method thus lower …

Geology Of The Tierras Blancas Area In The Southeastern Acambay Graben, Central Mexico, Lonnie T. Mercer

Theses and Dissertations

Pliocene-Pleistocene sediments in the southeastern Acambay graben, central Mexico have yielded mammal fossils, including Equus simplicidens, cf. Rhynchotherium, ?Camelops, Mammuthus sp., Bison sp., and Antilocapra sp. The fossiliferous sediments include a period of lacustrine sedimentation in the late Pliocene-early Pleistocene that interrupted fluvial and alluvial sedimentation during the early Pliocene and Pleistocene. The sediments deposited in this late Pliocene paleolake record a history of lake level fluctuations, shown by lithologic variations in lacustrine sediments and abundance of vertebrate burrows. Volcanic and tectonic events in the Acambay graben were the major controls on sedimentation during Pliocene-Pleistocene time. …

Biochemical Composition Of Particles And Dissolved Organic Matter Slong An Estuarine Gradient: Sources And Implications For Dom Reactivity, Antonio Mannino, H. Rodger Harvey

OES Faculty Publications

The chemical composition of high molecular weight dissolved organic matter (DOM) and particulate organic matter (POM) was examined along the salinity gradient of the Delaware Estuary. DOM was collected and fractionated by tangential-flow ultrafiltration into 1-30 kDa (HDOM; high molecular weight) and 30 kDa to 0.2 μm (VHDOM; very high molecular weight) and compared to particles collected in parallel. Polysaccharides comprised 12-43% of particulate organic carbon (POC), 30-56% of VHDOM carbon, and 7.5-19% of HDOM carbon. Hydrolyzable amino acids comprised 17-38% of POC, 5.4-12% of VHDOM carbon, and 1.5-4.2% of HDOM carbon. Only 7-43% of dissolved organic nitrogen in VHDOM …

Effects Of Ultraviolet Radiation On Attached Diatom Growth And Distribution, Heather Lynn Emsley Hatsell

School of Natural Resources: Dissertations, Theses, and Student Research

No abstract provided.

Regulation Of Algal Blooms In Antarctic Shelf Waters By The Release Of Iron From Melting Sea Ice, Peter N. Sedwick, Giacomo R. Ditullio

OES Faculty Publications

During summer 1995-96, we measured iron in the water column and conducted iron-enrichment bottle-incubation experiments at a station in the central Ross Sea (76°30'S, 170°40'W), first, in the presence of melting sea ice, and 17 days later, in ice-free conditions. We observed a striking temporal change in mixed-layer dissolved iron concentrations at this station, from 0.72-2.3 nM with sea ice present, to 0.16-0.17 nM in ice-free conditions. These changes were accompanied doubling of algal (diatom) biomass. Our incubation experiments suggest that conditions were iron-replete in the presence of sea ice, and iron-deficient in the absence of sea ice. We surmise …