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Full-Text Articles in Physical Sciences and Mathematics
Reply To "Comment On 'Kinetic-Energy Density Functional For A Special Shape-Invariant Potential Of A One-Dimensional Two-Level System'", Jiushu Shao, John J. Stezowski
Reply To "Comment On 'Kinetic-Energy Density Functional For A Special Shape-Invariant Potential Of A One-Dimensional Two-Level System'", Jiushu Shao, John J. Stezowski
John J. Stezowski Publications
For a harmonic potential, which is the special shape-invariant potential discussed in our paper [1], the relation between the ground state and the first-excited-state wave functions [Eq. (2)] should be . . .
Therefore, we have shown that one cannot find a closed form of the kinetic-energy density for the harmonic oscillator.
Use Of Affinity Chromatography In Developing Acridinium Ester-Labeled Antibodies For An Immunometric Assay Of Parathyrin, David S. Hage, Bob Taylor, Pat Schryver, Pai C. Kao
Use Of Affinity Chromatography In Developing Acridinium Ester-Labeled Antibodies For An Immunometric Assay Of Parathyrin, David S. Hage, Bob Taylor, Pat Schryver, Pai C. Kao
David Hage Publications
In developing an immunometric assay of intact parathyrin (parathyroid hormone, PTH), we found that affinity chromatography is a useful tool in purifying and optimizing the labeling conditions for acridinium ester-labeled antibodies. ... In summary, affinity chromatography was found to be useful in the purification of acridinium ester-labeled antibodies, particularly for removing denatured antibodies from the prepared label and for monitoring the amount of active labeled antibodies produced.
In Vitro Assembly Of Apophytochrome And Apophytochrome Deletion Mutants Expressed In Yeast With Phycocyanobilin, Lily Deforce, Ken-Ichi Tomizawa, Naoko Ito, Dave Farrens, Pill-Soon Song, Masaki Furuya
In Vitro Assembly Of Apophytochrome And Apophytochrome Deletion Mutants Expressed In Yeast With Phycocyanobilin, Lily Deforce, Ken-Ichi Tomizawa, Naoko Ito, Dave Farrens, Pill-Soon Song, Masaki Furuya
Chemistry Department: Faculty Publications
Recombinant pea type I phytochrome apoprotein expressed in yeast is shown to assemble in vitro with phycocyanobilin to produce a photoreversible phytochromelike adduct. As an initial investigation of the amino acid sequence requirements for chromophore incorporation, three phyA gene product deletion mutants were produced in yeast. Truncation of the N-terminal tail to residue 46 demonstrates that this region is not critical to bilin attachment, but a deletion mutant lacking 222 amino acids from the N terminus failed to yield holophytochrome in vitro, under the same conditions. A mutant comprising a deletion of the C terminus to residue 548 …