Physical Sciences and Mathematics Commons^™

Cloning, Overexpression, Purification And Characterization On N-Terminal Metal Binding Domains 5 And 6 Of Wilsons Disease Protein, David Achila

Masters Theses

Wilsons disease is an autosomal recessive disorder of copper metabolism which results from mutations in the gene encoding for Wilson disease protein (WDP). The cytosolic N-terminus of WDP comprises six metal binding domains each containing a conserved metal binding motif, GMXCXXC. The contribution of these metal binding domains to copper homeostasis is poorly understood. We have cloned, expressed and purified residues 485 to 633 corresponding to N-terminal metal-binding domains 5 and 6 of WDP (WD5-6). Two of the 3 disease causing mutations in the N-terminal metal binding domains of WDP occur one in each of these domains and the two …

Synthesis Of 123-Iodine Labeled Rofecoxib Analogues: Potential Nuclear Medicine Imaging Agents, Brandy U. Belue

Masters Theses

Single photon emission computed tomography (SPECT) is a medical imaging technique which provides three-dimensional images ofliving systems after introduction ofa radiolabeled pharmaceutical. Rofecoxib (4-[ 4-methylsulphonylphenyl]-3phenylfuran-2(SH)-one) is an inhibitor of the cycloxygenase-2 enzyme, an enzyme which has been found to promote cancer cell growth. The synthesis of a no-carrier-added iodine-123 labeled rofecoxib derivative is potentially of value for the detection of cancer utilizing SPECT.

Iodine-123 labeled -(3-Iodophenyl)-4-(methanesulfonylphenyl)-SH furan-2-one, 1, and 3-(4-Iodophenyl)-4-(methanesulfonylphenyl)-5H-furan-2-one, 2, were prepared from the respective potassium organotrifluoroborate salts. The trifluoroborates were prepared from a simple six step pathway.

Development Of A Dilatant Damage Zone Along A Thrust Relay In A Low-Porosity Quartz Arenite, Jennie E. Cook

Masters Theses

A damage zone developed along a backthrust fault system in well-cemented quartz arenite of the Tuscarora Sandstone in the Alleghanian foreland thrust system consists of a network of NW-dipping thrusts that are linked by multiple higher-order faults and bound a zone of intense extensional fractures and breccias. The damage zone is unusual in that it preserves porous brittle fabrics despite formation at >5km depth. The damage zone developed at an extensional step-over between two independent, laterally propagating backthrusts. Continued displacement resulted in breaching of the relay and formation of faultbounded horses, and favored the formation of extensional fractures. The presence …

Effect Of Surface Morphology On Adsorption-Induced Bending Of Microcantilevers, Ramya Desikan

Masters Theses

Microcantilevers undergo bending due to molecular adsorption when adsorption is confined to a single surface. The origin of the adsorption-induced force is assumed to be surface stress variation due to molecular adsorption. Single crystal silicon cantilevers were etched for a series of different time periods using two different types of Potassium Hydroxide solutions in order to obtain a rough and a smooth finish on the cantilever surface. Cantilevers that approximately had the same resonance frequency in the rough and smooth etched categories were chosen for comparison in the experiment. Liquid phase adsorption of 1-Do-decan-thiol on the cantilevers having a thin …

Detection Of Enteric Viruses In East Tennessee Public Ground Water Systems, Trisha Baldwin Johnson

Masters Theses

A two-part study was conducted by University of Tennessee-Knoxville, the U.S. Geological Survey, and the U.S. Environmental Protection Agency-National Exposure Research Laboratory to (1) develop, validate, and test a real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) assay for enteroviruses in ground water samples and to (2) perform the first survey of enteric viral occurrence in the karst aquifers of East Tennessee. Karst aquifers are expected to have a high susceptibility to viral contamination because of the rapid flow (100’s of m/day) and frequent occurrence of fecal indicator bacteria typically observed in these systems.

Real-time RT-PCR primers and probes specific for …

Development Of A Dilatant Damage Zone Along A Thrust Relay In A Low-Porosity Quartz Arenite, Jennie E. Cook

Masters Theses

A damage zone developed along a backthrust fault system in well-cemented quartz arenite of theTuscarora Sandstone in the Alleghanian foreland thrust system consists of a network of NW-dipping thrusts that are linked by multiple higher-order faults and bound a zone of intense extensional fractures and breccias. The damage zone is unusual in that it preserves porous brittle fabrics despite formation at >5km depth. The damage zone developed at an extensional step-over between two independent, laterally propagating backthrusts. Continued displacement resulted in breaching of the relay and formation of faultbounded horses, and favored the formation of extensional fractures. The presence of …

Deciphering Eustatic And Tectonic Influences During Parasequence Development In The Mesoprotozoic Helena/Wallace Formation, Belt Supergroup, Montana And Idaho, Stephen Alan Welch

Masters Theses

The stratigraphic architecture of sedimentary basins results from a combination of changes in relative sea-level and tectonism, and resulting changes in sediment supply. The Helena/Wallace formations, Mesoproterozoic Belt Supergroup, Montana and Idaho, consists of >500 meters of stacked meter-scale cycles (parasequences) that record in situ carbonate deposition as well as siliciclastic deposition from both the Laurentian craton and an unknown (tectonically active?) western source. In this study, statistical methods and 2-D forward modeling are combined with geochemical provenance analysis to examine parasequence stacking patterns and decipher the relative eustatic and tectonic controls on sequence development.

Helena/Wallace parasequences are typically composed …

Pedologic Investigations In The Red River Region Of Eastern Kentucky: Relationship Of Soil Properties To Lithology, Slope Aspect, And Soil Quality To Prehistoric Indigenous Food Plot Locations, Jason D. Windingstad

Masters Theses

ABSTRACT

Part I

The Red River Valley of Eastern Kentucky contains cultural remains that point to the independent development of agriculture during the Late Archaic cultural period. The objective of this study is to gather quantitative data through the chemical and physical analysis of soils collected within a valley transect to gain insights into landscape processes and soil quality that may have played a role in the development of plant domestication. National Cooperative Soil Survey standards were used for field descriptions and the chemical and physical analysis of all samples. Soil quality values such as ECEC (ammonium acetate pH 7), …

Stratigraphic And Structural Relationships Of The Ocoee Supergroup, Southern Appalachians: Implications For Neoproterzoic Rift Basin Architecture And Paleozoic Collisional Orogenesis, James Ryan Thigpen

Masters Theses

The late Proterozoic-Early Cambrian Ocoee Supergroup (OSG)is the dominant lithostratigraphic sequence of the western Blue Ridge (WBR) province in southeast Tennessee, southwest North Carolina, and northern Georgia. The OSG is divided into the basal Snowbird Group (SG) that nonconformably overlies Grenvillian basement, the thick Great Smoky Group (GSG) that is usually in fault contact with the Snowbird Group, and the Walden Creek Group (WCG) that directly underlies the Cambrian Chilhowee Group and conformably overlies both the Snowbird and Great Smoky Groups. Traditional interpretations suggest that the OSG was deposited during late Neoproterozoic-Early Cambrian rifting along the southeast Laurentian margin, initially …

Identification Of Terrestrial Alkalic Rocks Using Thermal Emission Spectroscopy: Applications To Martian Remote Sensing, Tasha Laurrelle Dunn

Masters Theses

We present a detailed study examining the use of laboratory thermal emission spectra (5-25 μm at 2 cm^-1 spectral sampling) for identification and classification of alkalic volcanic rocks. Modal mineralogies and derived bulk rock chemistries of a suite of terrestrial alkali basalts, trachyandesites, trachytes, and rhyolites were determined using linear spectral deconvolution. Model-derived mineral modes were compared to modes measured using an electron microprobe mapping technique to access the accuracy of linear deconvolution in determining mineral abundances. Standard deviations of 1σ of absolute differences between measured and modeled mineral abundances range from 0.68 to 15.02 vol %, with …

The Development Of Computational Tools For Halo Analysis And Study Of Halo Growth In The Spallation Neutron Source Linear Accelerator, Dirk A. Bartkoski

Masters Theses

The Spallation Neutron Source (SNS), being built at the Oak Ridge National Laboratory, utilizes a high intensity particle accelerator for neutron production. One of the challenges in SNS, as with all high intensity accelerators, is to minimize the amount of beam lost. High beam losses can cause costly damage and lead to residual activation of accelerator components, which complicates routine maintenance.

One of the key components in beam loss at SNS is the development and propagation of beam halo. Halo particles are those driven to large amplitudes by space charge forces between the beam particles or by mismatch between the …

Seasonal Forage Availability And Diet Of Reintroduced Elk In The Cumberland Mountains, Tennessee, Jason Lee Lupardus

Masters Theses

The Tennessee Wildlife Resources Agency (TWRA) reintroduced elk (Cervus elaphus manitobensis) into the Cumberland Mountains, Tennessee over a 3-year period beginning in December 2000. We radio-collared 160 elk and monitored them by aerial telemetry from February 2001 to June 2003. Locations (n = 1450) were used in a geographic information system (GIS) to develop a core herd home range (789-ha sampling area) to assess elk seasonal forage use and availability. We monitored diet and resource availability from November 2003 to October 2004 by vegetation sampling and microhistological analysis of feces. Tall fescue (Festuca arundinacea; 35.1%) dominated …

Applications Of Genetic Algorithms To A Variety Of Problems In Physics And Astronomy, Kevin Richard Williams

Masters Theses

Genetic algorithms are search techniques that borrow ideas from the biological process of evolution. By means of natural selection, genetic algorithms can be employed as robust numerical optimizers on problems that would normally be extremely problematic due to ill-behaved search spaces. The genetic algorithm has an advantage in that it is a global optimization strategy, as opposed to more conventional methods, which will often terminate at local maxima.

The success and resourcefulness of genetic algorithms as problem-solving strategies are quickly gaining recognition among researchers of diverse areas of study. In this thesis I elaborate on applications of a genetic algorithm …

Development Of A Web-Based Gis For Groundwater Exploration In Arid Lands, Nakul Manocha

Masters Theses

A three-fold exercise was conducted to assess the groundwater potentiality in the Eastern Desert (ED) of Egypt. First, a database was generated to host all relevant data sets in a GIS environment for a better understanding of the spatial relationships between these data sets. Co-registered digital mosaics were generated from relevant data sets including remote sensing (e.g., Landsat TM, SIR-C, SRTM, TRMM), geochemical (solute concentrations, O and H stable isotope composition), geological (geologic maps), and hydrological (lithology, depth to water table) data sets. Second, a web-based GIS interface (ArcIMS) was developed to provide a vehicle for data analysis, visualization, and …

Obtaining High Precision Results From Low Precision Hardware, Adam Donald Graham

Masters Theses

This document describes an attempt at acheiving high precision matrix multiplication results from the Lenslet EnLight256 Optical Signal Processor (OSP), which on its own can only produce results which are hardware limited to 8-bit signed integers. Due to it’s low precision, it’s has only limited applicability to real world problems, and if higher precision results were possible from the machine it could be used for more applications. A C library is developed for this thesis to allow high-precision results from the EnLight256. The library is described and results are given. Finally an implementation of the Jacobi Method on the EnLight256 …

Systems For Predicting Pitzer’S Ion-Interaction Model Parameters, Theresa Balderas Obias

Masters Theses

Pitzer's Ion-interaction Model had been widely used in predicting the non-ideal behavior of solution over a wide range of conditions for natural water systems. The model is successful only when there are sufficient parameters available. However, generating these needed parameters requires a great quantity of work and it would be advantageous to be able to predict the Pitzer's parameters for salt systems when there is no data available.

This research entailed the development of ionic radii dependent equations for Pitzer's binary ion-interaction parameters β_MX⁽⁰⁾, β_MX⁽¹⁾, β_MX⁽²⁾, and C^ϕ_MX . …

Universal Geometric Camera Calibration With Statistical Model Selection, Chistopher Paul Broaddus

Masters Theses

We propose a new universal camera calibration approach that uses statistical information criteria for automatic camera model selection. It requires the camera to observe a planar pattern from different positions, and then closed-form estimates for the intrinsic and extrinsic parameters are computed followed by nonlinear optimization. In lieu of modeling radial distortion, the lens projection of the camera is modeled, and in addition we include decentering distortion. This approach is particularly advantageous for wide angle (fisheye) camera calibration because it often reduces the complexity of the model compared to modeling radial distortion. We then apply statistical information criteria to automatically …

Virus Transport During Transient Unsaturated And Steady-State Saturated Flow Conditions In Memphis Aquifer Sand, Andrew Boruff Kenst

Masters Theses

The overall goal of this research was to determine the effect of transient unsaturated flow conditions on the transport of a virus in aquifer material. It was hypothesized that viruses would be transported at the same rate and over the same distance as the migration of the wetting front and that virus retention during transient unsaturated flow would be similar to that during steady-state saturated flow. Virus transport during transient unsaturated horizontal flow was experimentally compared with its behavior under steady-state saturated vertical flow. In the transient flow experiment, virus (ΦX174) suspension was introduced into an initially air-dry repacked Memphis …

Structural And Stratigraphic Investigations Of The Bays Mountain Synclinorium, Parrottsville And A Portion Of Cedar Creek 7.5-Minute Quadrangles, East Tennessee, Neil E. Whitmer

Masters Theses

The southern Valley and Ridge foreland fold-thrust belt is comprised of a wedge of Lower Cambrian through Pennsylvanian sedimentary rocks that were folded and faulted during the late stages of the Alleghanian orogeny. Within one of the eastern thrust sheets lies the Bays Mountain synclinorium. Rocks as young as Middle Ordovician are preserved in the core of the synclinorium and record the evolution of a Taconian (Blountian) Sevier tectonic basin.

The Parrottsville and Cedar Creek 7.5-minute quadrangles are located on the southeastern flank of the Bays Mountain synclinorium of East Tennessee and contain rocks belonging to the Conasauga, Knox, and …

A Reliability Case Study On Estimating Extremely Small Percentiles Of Strength Data For The Continuous Improvement Of Medium Density Fiberboard Product Quality, Weiwei Chen

Masters Theses

The objective of this thesis is to better estimate extremely small percentiles of strength distributions for measuring failure process in continuous improvement initiatives. These percentiles are of great interest for companies, oversight organizations, and consumers concerned with product safety and reliability. The thesis investigates the lower percentiles for the quality of medium density fiberboard (MDF). The international industrial standard for measuring quality for MDF is internal bond (IB, a tensile strength test). The results of the thesis indicated that the smaller percentiles are crucial, especially the first percentile and lower ones.

The thesis starts by introducing the background, study objectives, …

Investigating Nickel Partitioning In A Contaminant Aquifer, Suama Ndengu

Masters Theses

Improved prediction of the transport and bioavailability of metals in contaminated sediments demands a better quantitative understanding of how metals partition between aqueous solutions and mineral phases. This research examines the partitioning of nickel in a contaminated aquifer from Grand Rapids, Ml. Contaminated aquifer sediments were examined using the five step Tessier et al. (1979) operationally defined sequential extraction procedure to determine the solid phase distribution of nickel in the aquifer sediments. Models used to guide remediation efforts at the contaminated site assume that Ni behavior can be predicted based on Ni adsorption onto ferrihydrite (HFO). Although sequential extraction results …

Resistivity, Induced Polarization, And Spontaneous Potential Study Of Part Of The Calvin 28 Oil Field, Cass County Michigan, Eric M. Larsen

Masters Theses

Some hydrocarbon reservoirs have a documented reducing chimney, extending upward to the surface due to diffusion of hydrocarbon volatiles. This effect reportedly reduces the iron in the soil to magnetite and precipitates fine-grained pyrite if there is sufficient sulfur in the system. This study was conducted to test the detectability of the hypothetical chimney, using electrical geophysics in an environment of relatively recent glacial cover, known depth to an underlying petroleum reservoir, and known faulting.

The Calvin 28 oil field, in Cass County Michigan, is a middle Devonian domal field where reservoir rocks are draped over the central uplift block …

Structure-Activity Relationship Studies On Thioxolone Compounds As Inhibitors Of Human Carbonic Anhydrase Ii, Jared Nyabuto Orwenyo

Masters Theses

Carbonic anhydrase II (CA II) is a zinc metalloenzyme that catalyzes the reversible hydration of carbon dioxide. The role of this reaction in physiology has made it a target of many therapeutic approaches. Most CA II inhibitors currently known are sulfonamide-based and this is the only functional group incorporated into rationally designed CA II inhibitors. Thioxolone (6-hydroxy-1, 3-benzoxathiol-2-one), is a non-sulfonamide that has been found to have CA II inhibitory activity.

Thioxolone and its analogs were studied to evaluate the inhibition activity that can be achieved upon modification of the thioxolone molecule. IC₅₀ values and dissociation constants were determined …

Enhanced Performance Monitoring - Extending Papi To Monitor Multiple Counter Domains, Kevin Scott London

Masters Theses

High performance computing is playing an increasingly important role in the scientific community. As simulations start replacing physical experiments there will be a need for larger computing resources and better optimized applications. The Performance Application Programming Interface (PAPI) already addresses the concerns for optimizing a serial application by providing a portable interface to the performance counters found on modern processors. Developers can use these counters to understand exactly how their application is running on the processor and use this information to determine the best way to optimize the application. This information is only a portion of the complete picture. As …

The Classification Of Links Of Up To And Including Thirteen Crossings, Dylan Joshua Faullin

Masters Theses

The prime links of up to 13 crossings are classified up to unoriented equivalence.

Automatic Service Discovery For Grid Computing: A Decentralized Approach To The Grid, Jeffrey Michael Larkin

Masters Theses

The purpose of this document is to explore the feasibility of a decentralized system for grid computing. A proof-of-concept grid computing system was created based on the GridSolve computing environment and multicast domain name server with service discovery. This experimental grid computing environment showed that reasonable results can be achieved in a decentralized environment. Using these results suggestions are made for future research in this area.

Design, Synthesis And Characterization Of Nanosensors For Nerve Gas Agents, Thushara Gunasinghe

Masters Theses

This research is focused on the design synthesis and characterization of nanosensors to detect the nerve gas agents such as sarin at very low concentration levels (parts per billion and lower) which is very important to combat terrorism. Nanosensors constructed by the bottom up approach could provide the low sensitivity levels and high selectivity required for nerve gas detection. The sensing mechanism exploits the inherent properties of nanomaterials and signal amplification by signal transduction. The components of a nanoparticle-fluorescent monomer-nanomolecule-receptor (NMNR) sensor have been designed, synthesized, and characterized successfully. Interaction of the components of NMNR sensor with a model nerve …

A Tool Supporting Validation Of Uml Models, Weng Liong Low

Masters Theses

Software design is an important phase in the computer software development life cycle. The Unified Modeling Language (UML) is a widely used notation to design software models. As a software system increases in size and complexity it is harder for developers to check that their UML models are correct with respect to the UML metamodel. Consistency among different diagrams in a UML model is also important to ensure that there are no design conflicts that will lead to problems later. Incorrect software design will result in loss of productivity, time and money as developers fix design errors. Therefore, to ensure …

Applications Of Integrated Data Viewer – Idv In An Introductory Meteorology Class For Non-Science Majors, Ricardo C. Nogueria

Masters Theses

Computer products developed to visualize and integrate meteorological products with real-time data can create a virtual reality of the atmosphere. These advanced technologies help educators to improve teaching techniques in the classroom for science and non-science majors and increase the students' interest in learning as well. One of these products, the Integrated Data Viewer (IDV), was recently developed by University Corporation for Atmospheric Research (UCAR) project, Unidata, to visualize and integrate geoscience data. This study compares students' performance in the laboratory sessions using IDV-based and MANUAL-based exercises. Comparison of lab scores with exam scores of questions on the same topic …

Ionic Liquid As Catalysts In The Cycloaddition Of Co2 To Styrene Oxide In Supercritical And Subcritical States, Joy Mwalimu

Masters Theses

Supercritical fluids (SCFs) have fascinated researchers ever since the discovery of a critical temperature more than a century ago.¹ Initial studies focused mainly on the physical properties of supercritical phases.² However, their use as reaction media has sparked mainly during the last five to ten years.²

The coupling of CO₂ and Ionic Liquids (ILs) in the synthesis of cyclic carbonates comes from an increasing need for environmentally and toxicologically benign processes for the production of high-value compounds. In my research, CO₂ was utilized as both a replacement solvent and a C₁ feedstock in its …