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Full-Text Articles in Physical Sciences and Mathematics
Isolation And Characterization Of Novel Arginine-Derived Heterocyclic Amines, Nathaniel Srikureja
Isolation And Characterization Of Novel Arginine-Derived Heterocyclic Amines, Nathaniel Srikureja
Honors Theses
Most known heterocyclic amines (HCAs) are potent mutagens present at significant concentrations in cooked meats, and can be synthesized from burned mixtures of L-creatin(in)e with various amino acids. We hypothesize that novel mutagenic HCAs may be synthesized from a heated plant-based food simulating binary amino acid mL'\:ture: L-arginine and L-threonine. A two-part solid phase extraction was used to separate nitrogen heterocycles from byproducts followed by prep HPLC and the Ames mutagenicity assay using Salmonella TA98 to determine the mutagenicity of various isolates. The results indicate that this binary amino acid mixture does not yield a mutagenic sample.
Evaluating Mutagenicity Of Burned Arginine-Based Heterocyclic Amines And Anti-Mutagenicity Effect Of Chinese Medicinal Herbs, Rayford C. Alva
Evaluating Mutagenicity Of Burned Arginine-Based Heterocyclic Amines And Anti-Mutagenicity Effect Of Chinese Medicinal Herbs, Rayford C. Alva
Honors Theses
From the unexpected finding that cooked grains and meat substitutes elicit a mutagenic response in Salmonella typhimurium TA98, our work has been aimed at deconstructing this finding via a survey of heated binary amino acid combinations, involving arginine, a plant-based amino acid. secondly, our work has looked towards sing phytochemical extracts from traditional Chinese medicinal herbs to inhibit the mutagenic activity of heterocyclic amines (main culprit for mutagenicity in meat). Two fractions from the burned products of arginine and phenylalanine (RF-HCA-06 and -07) were statistically significant inducers of mutagenesis; Scutellaria barbata and Oldenlandia diffusa, both separately and together, were statistically …
Studies On Vinamidinium Salt Amine Exchange Reactions, Borohydride Reductions, And Subsequent Transformations, Xian Jia
Honors Theses
This research would not have been possible without the extraordinary guidance of Dr. John Gupton who I like to think of as the brains behind the operation. In addition, Dr. Nakul Telang, a postdoctoral fellow in Dr. Gupton’s research lab, has provided great support, assistance and humor throughout my undergraduate research career. The other student members of the lab that I would like to recognize for their support and contribution to this project are Ben Giglio, James Eaton, Peter Barelli, Mona Hovaizi, Kayleigh Hall, Scott Welden, Kara Finzel. We also thank the National Institutes of Health (grant no. R15‐CA67236) for …