Physical Sciences and Mathematics Commons^™

Identification Of Biomarkers That Distinguish Chemical Contaminants Based On Gene Expression Profiles, Xiaomou Wei, Junmei Ai, Youping Deng, Xin Guan, David R. Johnson, Choo Y. Ang, Chaoyang Zhang, Edward J. Perkins

Faculty Publications

Background: High throughput transcriptomics profiles such as those generated using microarrays have been useful in identifying biomarkers for different classification and toxicity prediction purposes. Here, we investigated the use of microarrays to predict chemical toxicants and their possible mechanisms of action.

Results: In this study, in vitro cultures of primary rat hepatocytes were exposed to 105 chemicals and vehicle controls, representing 14 compound classes. We comprehensively compared various normalization of gene expression profiles, feature selection and classification algorithms for the classification of these 105 chemicals into14 compound classes. We found that normalization had little effect on the averaged …

Survival Analysis With High-Dimensional Covariates: An Application In Microarray Studies, David Engler, Yi Li

Faculty Publications

Use of microarray technology often leads to high-dimensional and low-sample size (HDLSS) data settings. A variety of approaches have been proposed for variable selection in this context. However, only a small number of these have been adapted for time-to-event data where censoring is present. Among standard variable selection methods shown both to have good predictive accuracy and to be computationally efficient is the elastic net penalization approach. In this paper, adaptations of the elastic net approach are presented for variable selection both under the Cox proportional hazards model and under an accelerated failure time (AFT) model. Assessment of the two …