Physical Sciences and Mathematics Commons^™

Locating Hot Spots Of Fecal Pollution In An Urban Watershed Of Central Kentucky Using Bacteroides 16s Rrna Markers, Tricia L. Coakley

Theses and Dissertations--Earth and Environmental Sciences

The field of molecular fecal source tracking in the water environment has developed rapidly since the first PCR assays for general and host-­‐specific Bacteroides 16s rRNA markers were published. Numerous host-­‐specific molecular markers and PCR assays have been developed, adding greater specificity, sensitivity and quantitative methods to the array of options. The public demand for readying methods for transfer to the commercial lab, so that they may be used to generate data for public utilities, citizen action groups and regulatory agencies, has fueled the development of an entire new research community. These methods, however plentiful, have not found community agreement …