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Full-Text Articles in Physical Sciences and Mathematics
Fate Of Stable Isotope Label During Predation Of 15N-Tagged Wild-Type Escherichia Coli By Protozoa, Ashley M. Barton
Fate Of Stable Isotope Label During Predation Of 15N-Tagged Wild-Type Escherichia Coli By Protozoa, Ashley M. Barton
University of Kentucky Master's Theses
Currently, bacterial movement in karst aquifers is not well understood. Use of stable isotopes to label non-pathogenic Escherichia coli as a particulate groundwater tracer in karst systems has been examined in previous studies. Loss of the stable isotope signal is anticipated in traces greater than 500 m in length. Potential loss of 15N due to predation by protozoa was examined. Filter-sterilized water from Royal Spring in Georgetown, Kentucky, was inoculated with a mixture of either Tetrahymena pyriformis or Colpoda steinii and 15N-enriched E. coli and stored in the dark at 14°C. Samples were analyzed for their nitrogen isotope …
Chemical Evolution And Residence Time Of Groundwater In The Wilcox Aquifer Of The Northern Gulf Coastal Plain, Estifanos Haile
Chemical Evolution And Residence Time Of Groundwater In The Wilcox Aquifer Of The Northern Gulf Coastal Plain, Estifanos Haile
Theses and Dissertations--Earth and Environmental Sciences
This study aims to integrate groundwater geochemistry and mathematical modeling to determine the dominant geochemical processes and groundwater residence time within the Wilcox aquifer in the northern Gulf Coastal Plain. Groundwater samples were collected and analyzed for major ion chemistry, stable isotopes (18O, 2H, and 13C), and radioisotope 36Cl content. Geochemical modeling enabled the identification of major sources and sinks of solutes in the aquifer. A two-dimensional, finite-difference, numerical model was used to determine the deep groundwater flow rate and transport of 36Cl in the aquifer. Major ion chemistry shows a chromatographic pattern along …
Locating Hot Spots Of Fecal Pollution In An Urban Watershed Of Central Kentucky Using Bacteroides 16s Rrna Markers, Tricia L. Coakley
Locating Hot Spots Of Fecal Pollution In An Urban Watershed Of Central Kentucky Using Bacteroides 16s Rrna Markers, Tricia L. Coakley
Theses and Dissertations--Earth and Environmental Sciences
The field of molecular fecal source tracking in the water environment has developed rapidly since the first PCR assays for general and host-‐specific Bacteroides 16s rRNA markers were published. Numerous host-‐specific molecular markers and PCR assays have been developed, adding greater specificity, sensitivity and quantitative methods to the array of options. The public demand for readying methods for transfer to the commercial lab, so that they may be used to generate data for public utilities, citizen action groups and regulatory agencies, has fueled the development of an entire new research community. These methods, however plentiful, have not found community agreement …