Physical Sciences and Mathematics Commons^™

Synthesis Of 5-Methoxy-7-Methylbicyclo[3.2.0]Hept-2-En-6-Ol, Harlan Reid

Theses, Dissertations and Capstones

In order to generate a chiral metallocene ligand similar to Kaminsky-Sinn catalysts, a multistep synthesis for the production of 6,7-dimethylbicyclo[3.2.0]hepta-1,3-dienyl anion was proposed. Experiments directed at the initial phase of the proposal are included, ending with the characterization of new alcohols in compounds 9 through 12. Two steps from the synthesis were successfully combined, leading to improved recovery of the alcohols.

Administrative Perspectives On Technology Integration: The Globaloria: Myglife Program In West Virginia, William E. Chapman Jr.

Theses, Dissertations and Capstones

The reputed benefits of using technology in schools have been the topic of many research studies. When the World Wide Workshop Foundation implemented their Globaloria program into a group of pilot public secondary schools in West Virginia in the fall of 2008, another opportunity for study was created. The perceptions of principals relating to: 1) the effect(s) of the Globaloria program on students‘ academic outcomes; 2) the purpose(s) of the program; 3) the principal‘s role in the program 4) the benefits; and/or 5) the liabilities of having the program in their schools were examined in this mixed-method study. Analysis of …

A Gis Analysis On Possible Photovoltaic Cell Use For Energy Reduction During Peak Hours In Huntington, West Virginia, James Eric Tadlock

Theses, Dissertations and Capstones

Solar panels are one of the fastest growing renewable energy technologies. This study aims to identify to what extent roof-mounted solar panels can reduce the need of power provided by Appalachian Power Company. Data from the Reliability First Corporation was employed to determine the individual average household power usage. Three study areas in Huntington, West Virginia, were selected to determine if solar panels could be implemented. Roofs in the study areas were digitized to calculate the available area. Based on the average household usage, four different sized photovoltaic systems were determined. Potential power production was computed to identify any offset …

Finding Positive Solutions Of Boundary Value Dynamic Equations On Time Scale, Olusegun Michael Otunuga

Theses, Dissertations and Capstones

This thesis is on the study of dynamic equations on time scale. Most often, the derivatives and anti-derivatives of functions are taken on the domain of real numbers, which cannot be used to solve some models like insect populations that are continuous while in season and then follow a difference scheme with variable step-size. They die out in winter, while the eggs are incubating or dormant; and then they hatch in a new season, giving rise to a non overlapping population. The general idea of my thesis is to find the conditions for having a positive solution of any boundary …

High Resolution Single Molecule Optical Localization Of Multiple Fluorophores On Dna Origami Constructs Fluorophores On Dna Origami Constructs, Anuradha Rajulapati

Theses, Dissertations and Capstones

An ongoing challenge in the development of nanoelectronics and nanophotonics is the nondestructive, high-resolution localization in space of single molecules and multi-molecular assemblies. The apparent barrier to the use of optical microscopy at the sub-100 nm scale is the well known Abbe Limit, the diffraction limit to resolution. This laboratory has adapted a technique called Single-molecule high resolution imaging with photobleaching (SHRIMP). We have developed methods for utilizing SHRIMP for the determination of the separation of two fluorophores in single DNA origami constructs.

DNA Origami is extremely useful because it can address nanocomponents down to 2nm separation. It should be …

The Transsulfuration Pathway Significantly Contributes To Glutathione Biosynthesis In Human Mammary Epithelial Cells, Andrea Del Pilar Belalcazar Maya

Theses, Dissertations and Capstones

Cellular methylation and antioxidant metabolism are linked by the transsulfuration pathway, which converts the methionine cycle intermediate homocysteine to cysteine, a precursor for glutathione biosynthesis, principally in hepatic cells. In mammals, the transsulfuration pathway has been identified in liver, kidney, pancreas and brain. To determine whether the pathway exists in mammary cells human breast adenocarcinoma cells (MCF-7) and normal mammary epithelial cells (HMEC) were labeled with ³⁵S-methionine for 24 hours following pre-treatment with a vehicle control, the cysteine biosynthesis inhibitor propargylglycine (PPG) or the gammaglutamyl cysteine synthesis inhibitor buthionine sulfoximine (BSO). Cell lysates were prepared and reacted with glutathione-S-transferase …

Modeled Red Spruce Distribution Response To Climatic Change In Monongahela National Forest, James Michael Stanton

Theses, Dissertations and Capstones

In Monongahela National Forest of West Virginia, red spruce grows in high-elevation island ecosystems that are particularly sensitive to changes in climatic conditions. The ecological niche modeling application Maxent was used to project the distribution response of red spruce to climatic change for the purposes of conservation planning. Red spruce distribution data was acquired from the United States Forest Service. Three sets of nineteen bioclimatic variables, corresponding to present, 2050, and 2080 conditions, were derived from 1961-1990 monthly temperature and precipitation means and the IPCC A2 emissions scenario of HadCM3. The modeling revealed rapidly diminishing red spruce habitat suitability from …

Twin Solutions Of Even Order Boundary Value Problems For Ordinary Differential Equations And Finite Difference Equations, Xun Sun

Theses, Dissertations and Capstones

The Avery-Henderson fixed-point theorem is first applied to obtain the existence of at least two positive solutions for the boundary value problem

(-1)ⁿy⁽²ⁿ⁾ = f(y); n = 1; 2; 3 ... and t 2 [0; 1];

with boundary conditions

y(2k)(0) = 0

y^(2k+1)(1) = 0 for k = 0; 1; 2 ... n - 1:

This theorem is subsequently used to obtain the existence of at least two positive solutions for the dynamic boundary value problem

(-1)^{n (2n)}u(k)g(u(k)); n = 1; 2; 3 .... and k (0; ... N);

with boundary conditions

(2k)u(0) …

Variable Shape Parameter Strategies In Radial Basis Function Methods, Derek Sturgill

Theses, Dissertations and Capstones

The Radial Basis Function (RBF) method is an important tool in the interpolation of multidimensional scattered data. The method has several important properties. One is the ability to handle sparse and scattered data points. Another property is its ability to interpolate in more than one dimension. Furthermore, the Radial Basis Function method provides phenomenal accuracy which has made it very popular in many fields. Some examples of applications using the RBF method are numerical solutions to partial differential equations, image processing, and cartography. This thesis involves researching Radial Basis Functions using different shape parameter strategies. First, we introduce the Radial …

Structural Determination Of The 5' Untranslated Regions Of Ire-Containing Mrnas, Chandra Sekhar Bathina

Theses, Dissertations and Capstones

The expression of ferritin and amyloid precursor protein (APP) is post-transcriptionally regulated by iron-regulating proteins via binding to a stem-loop structure known as an iron-responsive element in the 5’-untranslated region (5’UTR) of ferritin and APP mRNAs. In this study, we used atomic force microscopy (AFM) to visualize the conformation of the 5’UTRs of ferritin heavy chain (Ferritin-H), ferritin light chain (Ferritin-L), and APP mRNA transcripts from human and mouse, and determined the secondary RNA structures using selective 2’-hydroxyl acylation analyzed by primer extension (SHAPE). The AFM imaging did not provide high resolution structural information about these RNAs, whereas the SHAPE …