Physical Sciences and Mathematics Commons^™

Development Of A Methodology For The Quantification Of Reaerosolization Of A Biological Contaminate Surrogate Particle From A Military Uniform Fabric, George Cooksey, Jeremy M. Slagley, Casey W. Cooper, Douglas Lewis, Alisha Helm

Faculty Publications

In a mass casualty medical evacuation after a bioaerosol (BA) dispersal event, a decontamination (DC) method is needed that can both decontaminate and prevent biological particle (BP) re-aerosolization (RA) of contaminated clothes. However, neither the efficacy of current DC methods nor the risk of BP RA is greatly explored in the existing literature. The goals of this study were to develop a repeatable method to quantify the RA of a biological contaminant off military uniform fabric swatches and to test the efficacy of one DC protocol (high-volume, low-pressure water) using 1 µm polystyrene latex (PSL) spheres as a surrogate. A …

Structural Engineering Of Thermostable Fluorescent Proteins Tgp-E And Ytp-E And Crystal Structure Of Tgp-E, Matthew Ryan Anderson

MSU Graduate Theses

Thermostable fluorescent proteins, such as thermal green protein (TGP) and yellow thermal protein (YTP), could be used as biosensors to monitor cellular activity and as a fusion tag to monitor a protein of interest. The use of fluorescent proteins can sometimes be limited in certain organelles with low pH and in thermophilic organisms. This research aims to improve the thermal stability of TGP and YTP. TGP was created from a synthetically derived eCGP123 protein to improve solubility by substituting residues on the positively charged b- barrel surface with negatively charged glutamate (E) at Los Alamos National Lab. YTP was developed …

Synthesis Of 7,12-Bis(4-(Di(1h-Pyrrol-2-Yl)Methyl)Phenyl)Benzo[K]Fluoranthene From Anew Dialdehyde As A Novel Fluorometric Bis-Dipyrromethane Derivative, Faride Ranjbari, Salar Hemmati, Mohammad Reza Rashidi

Turkish Journal of Chemistry

Dipyrromethanes are useful mediator structures which can be used as a part of other molecules such as bis-porphyrins and their derivations. Various methods have been developed for their synthesis. This study presents the synthesis of a new bis- dipyrromethane, 7,12-bis(4-(di(1H-pyrrol-2-yl)methyl)phenyl)benzo[k]fluoranthene, using the Lewis acid catalyzed reaction between a new dialdehyde and pyrrole at room temperature. The UV spectroscopic and fluorometric properties of the final product and precursors were determined. The newly synthesized product with desirable UV spectroscopic and fluorometric properties has the potential to be applied as a part of bisporphyrins or it can be used for other purposes in …

Conjugated Polymer-Based Biomaterials Through Controlled Self-Assembly, Megan Twomey

FIU Electronic Theses and Dissertations

Synthetic polymeric materials have gained significant use as biological materials (biomaterials) in biomedical and pharmaceutical applications. As a result, a demand for well-defined polymers with tunable properties has emerged. The synthetic versatility of polymeric biomaterials allows the opportunity to understand the structure-property relationship of materials and their cellular interactions. A novel class of polymeric biomaterials are conjugated polymers (CPs), which possess desirable physicochemical and excellent photophysical properties, including inherent fluorescence. The synthetic versatility of CPs allows easy modification of the conjugated backbone to tune emission and side chain structures to adjust biocompatibility through increased water solubility, controlled biodegradability, and incorporation …

Development Of Transition Metal-Catalyzed Polymerization Reactions And Their Applications For The Preparation Of Well-Defined Conjugated Polymer Architectures, Carlos Anthony Chavez

LSU Doctoral Dissertations

The synthesis of conjugated polymers and design of complex materials based on them have become increasingly popular as the demand for low-cost efficient and processible optoelectronic and semiconducting devices escalates. While advanced applications of polymeric materials require some degree of sophistication, success can be achieved through careful consideration of polymerization techniques and methodologies. Controlling critical aspects of the polymerization process allows for synthesis of complex macromolecular architectures possessing unique and novel properties that may be worth of further comprehensive investigation. This dissertation primarily focuses on the development and preparation of well-defined hierarchical macromolecular architectures. The foundation of this work encompasses …

Ultra-Small Fluorescent Inorganic Nanoparticles For Bioimaging, Zhen Li, Qiao Sun, Yian Zhu, Bien Tan, Zhi Ping Xu, S X. Dou

Shi Xue Dou

The novel optical, electrical, and magnetic properties of ultra-small inorganic nanoparticles make them very attractive in diverse applications in the fields of health, clean and renewable energy, and environmental sustainability. This article comprehensively summarizes state-of-the-art fluorescence imaging using ultra-small nanoparticles as probes, including quantum dots, metal nanoclusters, carbon nanomaterials, up-conversion, and silicon nanomaterials.

Surface Functionalization And Bioconjugation Of Nanoparticles For Biomedical Applications, Longyan Chen

Electronic Thesis and Dissertation Repository

Colloidal inorganic nanoparticles (NPs) have been attracting considerable interest in biomedicine, from drug and gene delivery to imaging, sensing and diagnostics. It is essential to modify the surface of nanoparticles to have enhanced biocompatibility and functionality for the in vitro and in vivo applications, especially in delivering locally and recognizing biomolecules. Herein, the goal of this research work is to develop advanced NPs with well-tailored surface functionalities and/or bio-functionality for the applications in cell tracking and analytes detection.

In the first project, quantum dots incorporating with gelatin nanoparticles (QDs-GNPs) have been developed for bioimaging applications. Two different approaches have been …

Investigations Into The Fluorescent Covalent Labeling Of Biomolecules Utilizing Rhodamine Dyes, Electrophilic Leaving Groups And Mrna Display., Susan D. Selaya

Theses and Dissertations

The discovery of a method by which proteins of interest can selectively be labeled with a probe of choice intracellularly is a longstanding goal in chemical biology research. Conventional labeling techniques have utilized large domain tags but despite the development of small labeling molecules there have been no short peptide sequences known to covalently label a small molecule without the aid of an enzymatic process or metal chelation. We aimed to find a sequence of nucleophilic peptides that reacted covalently and specifically with electrophilic small labeling molecules using mRNA display. Our goal was to show that an electrophilic small labeling …

Synthesis, Characterization, And Theoretical Studies Of Fluorescent Gumbos And Nanogumbos, Chengfei Lu

LSU Doctoral Dissertations

In this dissertation, novel fluorescent materials, which are referred to as a group of uniform materials based on organic salts (GUMBOS) are synthesized, characterized, and studied for different applications. GUMBOS are solid state organic salts having melting points from 25°C to 250°C. Similar to ionic liquids, GUMBOS are highly tunable and can be designed for specific applications by incorporating multiple-functions into a single ion pair by selection of cation-anion combinations. Chapter 2 is a discussion of the synthesis and characterization of a PEGylated IR786 GUMBOS. These GUMBOS are prepared by a two-step synthesis procedure followed by a facile anion exchange …

Ultra-Small Fluorescent Inorganic Nanoparticles For Bioimaging, Zhen Li, Qiao Sun, Yian Zhu, Bien Tan, Zhi Ping Xu, S X. Dou

Australian Institute for Innovative Materials - Papers

The novel optical, electrical, and magnetic properties of ultra-small inorganic nanoparticles make them very attractive in diverse applications in the fields of health, clean and renewable energy, and environmental sustainability. This article comprehensively summarizes state-of-the-art fluorescence imaging using ultra-small nanoparticles as probes, including quantum dots, metal nanoclusters, carbon nanomaterials, up-conversion, and silicon nanomaterials.

Fluorescent Cytidine Analogues, Kirby J. Chicas

Electronic Thesis and Dissertation Repository

Three luminescent cytidine analogues have been synthesized in order to perform SNP analysis by fluorescence spectroscopy. Herein is described the synthesis of 6-pyrenylpyrrolocytidine (PypdC), its photophysical characterization, and its subsequent incorporation into oligodeoxynucleotides (ODNs). The behavior of PypdC in ODNs is described as well as fluorescence intensity changes with respect to the match and mismatch cases. In order to obtain a greater understanding of pyrene’s interaction with pyrrolocytidine, a congener, pyrenyl ethynyl cytidine (PyEtdC) was synthesized. The congener was photophysically studied and prepared for oligo synthesis. Finally, exploration into indole substituted pyrrolocytidines (pdCs) was undertaken to explore new pdC structures.

Silica Polypeptide-Based Colloids: Physical Properties And Novel Materials, Cornelia Rosu

LSU Doctoral Dissertations

The investigation of polypeptide composite particles, PCPs, is described. The production of mesoporous and polycolloid architectures is also addressed. PCPs were prepared by both growing form and grafting to method. They consist of a core made of silica, silica-coated magnetite or silica-coated cobalt. Some of the particles were covalently labeled with fluorescein isothiocyanate. Several polypeptides were chosen for attachment on the core: poly(γ-stearyl-L-glutamate), PSLG, poly(Nε-carbobenzyloxy-L-lysine), PCBL and poly(o-benzyl-L-tyrosine), PBTY. Attachment of a well-characterized alkyne-end terminated PSLG and PCBL to an azide- functionalized particle yielded PCPs with a desired sparse coverage. A blend of techniques such as FTIR, XPS, TEM, HTEM, …

Synthesis Of Fluorescent Poly(Styrene Sulfonate), Wayne Alfred Huberty

LSU Master's Theses

In 1978, Lee found an increase and then a large decrease in the diffusion coefficient of poly(lysine) as the concentration of salt is decreased (Lin, S. C.; Lee, W. I.; Schurr, J. M. Biopolymers 1978, 17, (4), 1041-1064). Since the “slow mode” discovery, many have studied it without finding a fully satisfactory answer. Additional dynamic light scattering (DLS) measurements have been the main experimental technique used for reinvestigating the slow mode decay. Although DLS is a powerful characterization tool, it depends on thermodynamic interactions. As complicated as they can be, thermodynamic interactions are much worse for polyelectrolytes because of the …

Development Of Boronic Acid Flurescent Reporters, Boronic Acid-Modified Thymidine Triphosphates For Sensor Design And Antagonists Of Bacterial Quorum Sensing In Vibrio Harveyi, Yunfeng Cheng

Chemistry Dissertations

Carbohydrates are known to play important roles in a large number of physiological and pathological processes. Conceivably, “binders” of carbohydrates of biological importance could be used as diagnostic and therapeutic agents. Currently, lectins are the major available tools in research for carbohydrate recognition. However, the available lectins often have cross-reactivity issues, along with the high costs and stability issues. Therefore, there is a critical need to develop alternatives (lectin mimics). In this regard, there have been very active efforts in developing different “binders”, such as small molecule lectinmimics and aptamers. Among all the small molecule lectinbmimics developments, boronic acid stands …

Development Of Luminescent Sensing Systems With Clinical Applications, Daniel F. Scott

University of Kentucky Doctoral Dissertations

As the move towards the miniaturization of many diagnostic and detection systems continues, the need for increasingly versatile yet sensitive labels for use in these systems also grows. Luminescent reporters provide us with a solution to many of the issues at hand through their unique and favorable characteristics. Bioluminescent proteins offer detection at extremely low concentrations and no interference from physiological fluids leading to excellent detection limits, while the vast number of fluorescent proteins and molecules available allows the opportunity to select a tailored reporter for a specific task. Both provide relatively simply instrumentation requirements and have exhibited great promise …

Yellow-Fluorescing Calcite From Sterling Hill, James A. Van Fleet, E. R. Verbeek

Faculty Journal Articles

No abstract provided.

The Electrical Performance Of Modern Compact Fluorescent Lamps, Sean T. Elphick, Philip Ciufo, Sarath Perera

Faculty of Informatics - Papers (Archive)

Compact Fluorescent Lamps (CFL) are highly non-linear devices which are likely to experience very high growth in penetration levels, especially in domestic environments, in Australia over the next 2 – 3 years. This will occur due to the decision taken by the Australian Federal Government to ban the sale of incandescent light globes after 2009 as measures towards meeting the needs for demand side management and climate change abatement. While CFL technology has been in existence since the late 1980s penetration levels are now increasing to a point where the total CFL load cannot be considered negligible compared to other …

Mass Marking Of Mosquitofish: Preliminary Results, Bruce Vondracek, Wayne A. Wurtsbaugh, Joseph J. Cech Jr.

Watershed Sciences Faculty Publications

Three marking techniques were tested to determine their applicability for mosquitofish. Tetracycline drugs and DC Ar administered in the diet successfully marked laboratory-cultured mosquitofish, but exposure to direct sunlight in outdoor tanks resulted in the rapid disappearance of the marks. Preliminary data on fluorescent marks from a polystyrene pigment in a melamine-sulfonamide-formaldehyde resin forced into the dermal ,tissue with compressed air are more promising.

A t an optimal deliver pressure of 7.3 m Hg (140 p.s.i.) and spraying time of 15 sec., marking percentage is maximized and fish mortality is minimized. Mark retention time was up to 80 days in …