Physical Sciences and Mathematics Commons^™

The Role Of Mannosyl-Phosphoryl-Dihydropolyisoprenol In The Synthesis Of Mammalian Glycoproteins, John W. Baynes, An-Fei Hsu, Edward C. Heath

Faculty Publications

A mouse myeloma tumor was used as a model system to study the biochemical steps involved in the incorporation of mannose into glycoproteins. This tumor, MOPC-46B, synthesizes a K-type immunoglobulin light chain (K-46) which is a glycoprotein with a single oligosaccharide side chain containing mannose as one of its constituent sugars. MOPC-46B microsomal preparations contain enzymes which transfer mannose from the sugar nucleotide, GDPmannose, to endogenous lipid and protein acceptors. Formation of the mannolipid proceeds by the reversible transfer of mannose from GDP-mannose to an endogenous phospholipid. The mannolipid was purified and characterized by chemical methods and mass spectrometry as …