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Full-Text Articles in Physical Sciences and Mathematics

Addressing Soil Carbon Sequestration Response From Multispecies Dairy Forage Systems And Modeling Rangeland Beef Cow Dry Matter Intake Using Precision Enteric Emissions Measurements, Lillian J. Mcfadden Jan 2023

Addressing Soil Carbon Sequestration Response From Multispecies Dairy Forage Systems And Modeling Rangeland Beef Cow Dry Matter Intake Using Precision Enteric Emissions Measurements, Lillian J. Mcfadden

Electronic Theses and Dissertations

Regenerative agriculture is a pressing matter for the dairy industry to address cropland sustainability and carbon sequestration. One regenerative management practice that has been identified to help with row crop sustainability for key metrics like soil organic carbon (SOC) is complex covers. When producers use complex covers one of the main challenges is that it takes time to detect a change in SOC. However, simulation models are a tool that can be used to help determine if a regenerative practice is a strategy that gives the best results (i.e., increased SOC) while aligning with long-term production goals. Therefore, our objectives …


Distribution And Soil Characteristics Associated With The Neches River Rose-Mallow (Hibiscus Dasycalyx), Morgan E. Langlinais Dec 2022

Distribution And Soil Characteristics Associated With The Neches River Rose-Mallow (Hibiscus Dasycalyx), Morgan E. Langlinais

Electronic Theses and Dissertations

The Neches River rose-mallow, Hibiscus dasycalyx, is a rare herbaceous perennial endemic to East Texas. The threatened, obligate wetland species is currently at risk of being lost in the wild due to habitat loss, encroachment of nonnative and native plant species, and hybridization with two other Hibiscus congeners, Hibiscus laevis and Hibiscus moscheutos. Known locations of each of the three Hibiscus species were used to measure select soil characteristics and habitat variables associated with each of the rose-mallows. H. dasycalyx was concluded to be associated with more fertile soils and slightly more canopy coverage than H. laevis and …


Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg Dec 2022

Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg

Theses and Dissertations

The work here presents two main parts. In the first part, chapters 1 – 3 focus on dynamical systems modeling in plant immunity, whereas chapters 4 – 6 describe contributions to computational modeling and analysis of proteomics and genomics data. Chapter 1 investigates dynamical and biochemical patterns of reversibly oxidized cysteines (RevOxCys) during effector-triggered immunity (ETI) in Arabidopsis, examines the regulatory patterns associated with Arabidopsis thimet oligopeptidase 1 and 2’s (TOP1 and TOP2), roles in the RevOxCys events during ETI, and analyzes the redox phenotype of the top1top2 mutant. The second chapter investigates the peptidome dynamics during ETI …


Insights On The Behavior Of Nano-Copper In The Agroecosystem: Mycorrhizal Associations With Spearmint (Mentha Spicata), Suzanne Annette Apodaca Dec 2022

Insights On The Behavior Of Nano-Copper In The Agroecosystem: Mycorrhizal Associations With Spearmint (Mentha Spicata), Suzanne Annette Apodaca

Open Access Theses & Dissertations

Nanotechnology offers significant potential benefits to our society, including the agriculture sector. With the advancement of nano-enabled agrochemicals towards sustainable and efficient agricultural practices, it is essential to address environmental issues associated with the use of nanoscale materials. The same properties that give promise to applications of nanotechnology in modern agriculture could have unintended consequences on ecosystem dynamics. A point of concern for risk management is the impact of engineered nanomaterials (ENMs) to beneficial microbial communities, which support a variety of ecosystem services.

Use of copper (Cu) products in agriculture are based on their abundance, role as a micronutrient, and …


South Texas Wildlife Activity Across A Fragmented Landscape And Road Mitigation Corridor, Caitlin K. Brett Dec 2022

South Texas Wildlife Activity Across A Fragmented Landscape And Road Mitigation Corridor, Caitlin K. Brett

Theses and Dissertations

Wildlife crossing structures (WCS) and roadside fencing are commonly installed to mitigate habitat fragmentation, wildlife road mortalities, and other negative effects that roads can have on the surrounding landscape. Eight such WCS were constructed below Farm-to-Market (FM)106 in Cameron County, Texas, across a 16 km corridor transecting the Laguna Atascosa National Wildlife Refuge. These WCS, paired with adjacent roadside fencing, were intended to prevent road mortalities of the endangered ocelot (Leopardus pardalis) and to mitigate the barrier effect of FM106 on this and other meso-mammal species. This study will analyze camera trap data from roadside and habitat reference …


Quantifying The Effect Of Disturbance On Native Mojave Desert Shrubs, Tamara J. Wynne Dec 2022

Quantifying The Effect Of Disturbance On Native Mojave Desert Shrubs, Tamara J. Wynne

UNLV Theses, Dissertations, Professional Papers, and Capstones

Disturbance can come in many different forms. In our studies, we looked at the impact of a solar photovoltaic facility on native plants growing inside and outside of the facility, alteration in precipitation (simulated) on four native shrubs and the impact of applying supplemental water as a function of volume and frequency to establish native shrubs such as might occur at restoration sites. Disturbance is becoming a more common phenomenon in many ecosystems throughout the world, increasing the need for studies that quantify the impact at the plant and ecosystem level. Each research project revealed different plant responses, such as …


Influence Of Pretreatments On Physiological Status And Post-Planting Survival Of Thornforest Seedlings In A Restoration Context, Douglas E. Mainhart Dec 2022

Influence Of Pretreatments On Physiological Status And Post-Planting Survival Of Thornforest Seedlings In A Restoration Context, Douglas E. Mainhart

Theses and Dissertations

Semi-arid ecosystems across the globe have faced land conversion posing a threat to the important ecosystem services they provide. Revegetation-centered restoration efforts in arid regions have low success due to limited water availability and higher temperatures. Typically, practitioners utilize methods that modify field conditions around seedlings to mitigate these stressors. I evaluated the efficacy of in-nursery treatments to improve plant water status. Three pretreatments were applied: elevated CO2, drought hardening, and an antitranspirant foliar spray. My goal was to understand how these pretreatments would impact survival and growth in the field and what physiological traits they altered. Elevated CO2 and …


Nitrogen Use Efficiency Of Pretassel Nitrogen Applications In Corn, Robyn Brittlee Mulloy Dec 2022

Nitrogen Use Efficiency Of Pretassel Nitrogen Applications In Corn, Robyn Brittlee Mulloy

Graduate Theses and Dissertations

Corn (Zea mays L.) production continues to be a critical component of row-crop production systems within Arkansas and is seeing a resurgence in recent years. Nitrogen (N) is critical for corn growth and is often one of the single largest input costs associated with corn production. Research objectives for this study were to determine the nitrogen use efficiency (NUE) of late season N applications as influenced by sidedress N rate and timing of pretassel application and to quantify the nutrient uptake and partitioning in modern era corn hybrids within a furrow-irrigated production system. Research was conducted at the Milo J. …


Nitrogen Management In Rice Under Suboptimal Soil Conditions, Payton Suzanne Rhea Dec 2022

Nitrogen Management In Rice Under Suboptimal Soil Conditions, Payton Suzanne Rhea

Graduate Theses and Dissertations

In Mid-South rice (Oryza sativa, L.) production, nitrogen (N) fertilizer management for pureline varieties is most often recommended as a single preflood (SPF) or two-way split (2WS) application in a direct-seeded, delayed-flood system. Most of the N fertilizer is typically applied at the four- to six-leaf stage onto dry soil, and the second application, if necessary, into the floodwater during early reproductive growth stages (referred to as midseason). Environmental factors frequently prohibit growers from applying early N fertilizer under optimal dry soil conditions. A study was conducted to determine the best N fertilization management practices to utilize in rice when …


Sustainability And Health Impacts Of Pulse Crops In The United States Using Life Cycle Assessment, Prathamesh A. Bandekar Dec 2022

Sustainability And Health Impacts Of Pulse Crops In The United States Using Life Cycle Assessment, Prathamesh A. Bandekar

Graduate Theses and Dissertations

Environmental sustainability and human health impact of pulses produced and consumed in the United States was assessed using life cycle assessment (LCA). The study included three objectives 1) to estimate environmental impact of current production and consumption practices in the United States using attributional LCA; 2) to estimate environmental and human health impact of iso-caloric diets containing varying amounts of pulses using Hybrid-LCA and Combined Nutritional and Environmental-LCA (CONE-LCA); and 3) to estimate environmental impact of increased demand for pulses using consequential LCA. Scope of the study varied for each objective with system boundary encompassing cradle-to-grave activities for objective 1 …


Diagnosing Excess Nitrogen In Rice Using Post-Season Tissue Samples, Kyle Hoegenauer Dec 2022

Diagnosing Excess Nitrogen In Rice Using Post-Season Tissue Samples, Kyle Hoegenauer

Graduate Theses and Dissertations

Proper nitrogen (N) management in rice production can be difficult to achieve without the aid of tools such as soil and tissue tests. There are no tests currently available to rice producers that determine whether N has been managed properly throughout the season or that detect instances of over fertilization. Rice stalk samples were collected from N response trials within 3 days of harvest from the primary Arkansas rice growing region in 2016-2018. Stalk samples were analyzed for N concentration through a KCl extraction and spectrofluorometric quantification. Samples analyzed with leaf material intact contained statistically different N concentrations than samples …


Using Soil Testing Data To Examine Organic Carbon Changes During The Past 27 Years In Maine Agricultural Soils, Andrew Chase Aug 2022

Using Soil Testing Data To Examine Organic Carbon Changes During The Past 27 Years In Maine Agricultural Soils, Andrew Chase

Electronic Theses and Dissertations

Agricultural soils may act as a source or a sink for carbon (C) in the global C cycle. With rising atmospheric CO2 levels, C sequestration in soils may play an important role in climate change mitigation. Soil organic carbon (SOC) also contributes to key aspects of soil health and fertility, such as aggregation, water-holding capacity, microbial biomass, and nutrient mineralization. Although SOC may be calculated from estimates of soil organic matter (SOM) obtained by loss-on-ignition (LOI), factors such as sample clay concentration and combustion temperature introduce error into estimates of SOM. We explored the potential for an extensive collection of …


Quantifying Spatial Heterogeneity Of Wild Blueberries And Crop Water Stress Monitoring Using Remote Sensing Technologies, Kallol Barai Aug 2022

Quantifying Spatial Heterogeneity Of Wild Blueberries And Crop Water Stress Monitoring Using Remote Sensing Technologies, Kallol Barai

Electronic Theses and Dissertations

The wild blueberry is one of the major crops of Maine, with significant economic value and potential health benefits. Due to global climate change, drought impacts have been increasing significantly in recent years in the northeast region of the USA, causing significant economic losses in the agricultural sectors. It has been predicted to increase further in the future. Changing patterns of the elevated atmospheric temperatures, increased rainfall variabilities, and more frequent drought events have made the wild blueberry industry of Maine vulnerable, suggesting the adoption of novel approaches to mitigate the negative impacts of global climate changes. Also, wild blueberry …


Classification Models For 2,4-D Formulations In Damaged Enlist Crops Through The Application Of Ftir Spectroscopy And Machine Learning Algorithms, Benjamin Blackburn Aug 2022

Classification Models For 2,4-D Formulations In Damaged Enlist Crops Through The Application Of Ftir Spectroscopy And Machine Learning Algorithms, Benjamin Blackburn

Theses and Dissertations

With new 2,4-Dichlorophenoxyacetic acid (2,4-D) tolerant crops, increases in off-target movement events are expected. New formulations may mitigate these events, but standard lab techniques are ineffective in identifying these 2,4-D formulations. Using Fourier-transform infrared spectroscopy and machine learning algorithms, research was conducted to classify 2,4-D formulations in treated herbicide-tolerant soybeans and cotton and observe the influence of leaf treatment status and collection timing on classification accuracy. Pooled Classification models using k-nearest neighbor classified 2,4-D formulations with over 65% accuracy in cotton and soybean. Tissue collected 14 DAT and 21 DAT for cotton and soybean respectively produced higher accuracies than the …


Determining The Effects Of Elevated Carbon Dioxide On Soil Acidification, Cation Depletion, And Soil Inorganic Carbon And Mapping Soil Carbons Using Artificial Intelligence, Jannatul Ferdush Aug 2022

Determining The Effects Of Elevated Carbon Dioxide On Soil Acidification, Cation Depletion, And Soil Inorganic Carbon And Mapping Soil Carbons Using Artificial Intelligence, Jannatul Ferdush

Theses and Dissertations

Soil carbon is the largest sink and source of the global carbon cycle and is disturbed by several natural, anthropogenic, and environmental factors. The global increase of atmospheric CO2 affects soil carbon cycling through varied biogeochemical processes. The first chapter is a compilation of current information on potential factors triggering soil acidification and weathering mechanisms under elevated CO2 and their consequences on soil inorganic carbon (SIC) pool and quality. Soil water content and precipitation were critical factors influencing elevated CO2 effects on the SIC pool. The second chapter examines a detailed column experiment in which six soils …


Better Understanding Genomic Architecture With The Use Of Applied Statistics And Explainable Artificial Intelligence, Jonathon C. Romero Aug 2022

Better Understanding Genomic Architecture With The Use Of Applied Statistics And Explainable Artificial Intelligence, Jonathon C. Romero

Doctoral Dissertations

With the continuous improvements in biological data collection, new techniques are needed to better understand the complex relationships in genomic and other biological data sets. Explainable Artificial Intelligence (X-AI) techniques like Iterative Random Forest (iRF) excel at finding interactions within data, such as genomic epistasis. Here, the introduction of new methods to mine for these complex interactions is shown in a variety of scenarios. The application of iRF as a method for Genomic Wide Epistasis Studies shows that the method is robust in finding interacting sets of features in synthetic data, without requiring the exponentially increasing computation time of many …


Chromosome Number Evolution, Phylogeography, And The Effects Of Climate Change On Species Distributions In Polyploid Plant Systems, Courtney H. Babin Aug 2022

Chromosome Number Evolution, Phylogeography, And The Effects Of Climate Change On Species Distributions In Polyploid Plant Systems, Courtney H. Babin

University of New Orleans Theses and Dissertations

Polyploidy, a term used to describe organisms with cells having more than two paired sets of chromosomes, is a significant driver of diversification among land plants. Over a century of research has advanced our understanding of polyploidization in some taxa, but polyploid organisms remain understudied. In this dissertation, I investigate chromosome number evolution, phylogeographic structure, genetic differentiation, and the effects of climate change on ploidy level distribution using polyploid plant systems. In the first chapter, I inferred a molecular phylogeny of Allium, an economically important genus that includes cultivated crops and ornamentals, to investigate evolutionary transitions in chromosome number …


Tree Ring Reconstructions Of Streamflow For The Lower Rio Grande Valley Of Texas, Adedolapo Mutiyat Adeyanju Aug 2022

Tree Ring Reconstructions Of Streamflow For The Lower Rio Grande Valley Of Texas, Adedolapo Mutiyat Adeyanju

Theses and Dissertations

The Rio Grande River is a major water source for people living within the USA-Mexico border. The Rio Grande River has its headwaters in the San Juan Mountains in Colorado and the Sierra Madre Occidental in Mexico before flowing into Texas through El-Paso. The water supply issues facing the Lower Rio Grande Basin (LRGB) are extremely complex from international restrictions to severe climate change. The river shares its flow between the U.S.A and Mexico based on the provisions of the 1944 treaty between the U.S.A and Mexico. The LRGB flow is regulated by releases from Falcon and Amistad Reservoirs managed …


Water Sourcing Strategies Of Desert Vegetation In Varying Soil Textures With Vegetation Competition: A Stable Isotope Analysis, Martha Elizabeth Gardea Aug 2022

Water Sourcing Strategies Of Desert Vegetation In Varying Soil Textures With Vegetation Competition: A Stable Isotope Analysis, Martha Elizabeth Gardea

Open Access Theses & Dissertations

Recent studies have suggested an ecohydrological separation of water exists in the northern Chihuahuan Desert, where vegetation types, rainfall regimes, and location in the terrain seem to determine the type of water (e.g. isotopically depleted or enriched in 18O and 2H) plants are able to source and use throughout their growing cycle. That work found evidence of creosote (Larrea tridentata) and mesquite (Prosopis glandulosa) using tightly bound soil water at both a site on an ephemeral channel and a site in higher elevated flat area because the isotopic signature of plant water at the beginning of the study did not …


Evaluation Of Factors That Contribute To Injury To Quizalofop-Resistant Rice From Quizalofop Applied Postemergence, Navdeep Godara Aug 2022

Evaluation Of Factors That Contribute To Injury To Quizalofop-Resistant Rice From Quizalofop Applied Postemergence, Navdeep Godara

Graduate Theses and Dissertations

Quizalofop-resistant rice technology allows for over-the-top applications of quizalofop, an acetyl-coenzyme A carboxylase-inhibiting herbicide. However, quizalofop caused significant injury to quizalofop-resistant rice in some Arkansas fields during the first year of commercialization. Experiments evaluated the effect of early-season soil moisture and nitrogen availability; pre-exposure to low rates of glyphosate and imazethapyr; planting date; and environmental conditions including, soil moisture content, air temperature, and light intensity on quizalofop-resistant rice tolerance to quizalofop applications. All experiments assessed sequential quizalofop applications made to 2-leaf followed by 5-leaf stage of rice. Sequential quizalofop applications alone and with surface irrigation or nitrogen application at the …


The Effect Of Water Management And Ratoon Rice Cropping On Methane Emissions And Harvest Yield In Arkansas, Marguerita Leavitt Aug 2022

The Effect Of Water Management And Ratoon Rice Cropping On Methane Emissions And Harvest Yield In Arkansas, Marguerita Leavitt

Graduate Theses and Dissertations

Sustainable intensification of rice farming is crucial to meeting human food needs while reducing environmental impacts. Rice produces 8% of all anthropogenic CH4, which is a potent greenhouse gas. CH4 emissions can potentially be reduced by cultivation practices that minimize the number of days the fields are saturated, such as dry-seeding instead of water-seeding and irrigation using the alternate wetting and drying (AWD) technique instead of delayed, continuous flooding (DF). Ratoon cropping, wherein a second crop of rice is grown from the harvested stubble of the first crop, can be used to produce additional yield with minimal labor, but may …


Optimization Techniques For Soil Organic Carbon Prediction Using Mid-Infrared Spectroscopy, Minerva J. Dorantes Aug 2022

Optimization Techniques For Soil Organic Carbon Prediction Using Mid-Infrared Spectroscopy, Minerva J. Dorantes

Graduate Theses and Dissertations

Resource-efficient techniques for accurate soil carbon estimation are necessary to satisfy the increasing demand for spatiotemporal data. In the last thirty years, mid-infrared (MIR) soil spectroscopy has developed as an accurate, rapid, cost-effective, and non-destructive technique for soil organic carbon (SOC) analysis. In soil spectroscopy, a calibration model relates spectral data to a corresponding measured soil property and is subsequently used to predict this value from new spectral data. Various optimization techniques have been used to improve the statistical performance of calibrations; however, there is little consensus on the conditions that make these techniques effective. The objectives of this research …


Assessing Consumer Demand, Producer Profitability, And The Environmental Impacts Of Conservation Agriculture Adoption In Sub-Saharan Africa, Willy Byamungu Mulimbi Aug 2022

Assessing Consumer Demand, Producer Profitability, And The Environmental Impacts Of Conservation Agriculture Adoption In Sub-Saharan Africa, Willy Byamungu Mulimbi

Graduate Theses and Dissertations

This dissertation explores three aspects of conservation agriculture (CA) in the Sub-Saharan African region (SSA). The first article examines the demand side of CA and explores whether urban maize (Zea mays L.) consumers in the Democratic Republic of the Congo (DRC) would be willing to pay a premium for CA-produced maize flour. The second article estimates the effects CA provides to adopters and their farms in smallholder farming systems in the DRC, focusing on changes in soil properties and cowpea (Vigna unguiculata) yields. The final article uses a Life Cycle Assessment (LCA) approach to monetize the environmental impacts of adopting …


Hiding In Plain Sight: Accounting For Rate Heterogeneity In Trait Evolution Models, James Boyko Aug 2022

Hiding In Plain Sight: Accounting For Rate Heterogeneity In Trait Evolution Models, James Boyko

Graduate Theses and Dissertations

Within the last four decades, phylogenetic comparative methods have become the defacto method of analysis for comparative biologists. The availability of high-quality comparative datasets has been matched by an explosion of possible phylogenetic models. In large part, the efforts to increase the realism of phylogenetic comparative methods has been successful as evidenced by their widespread use. To this extensive literature, my contributions are modest. I have focused my dissertation work on two main themes. First, most phenotypic evolution is not independent of other phenotypes. Changes in a particular character may influence changes in another and modeling these characters in isolation …


Orthotrichum Lyellii As An Active Moss Biomonitor: Examining The Interplay Between Ambient Pm10, Bulk Deposition And Heavy Metals In An Urban Environment, Scott Bradley Kiel Jul 2022

Orthotrichum Lyellii As An Active Moss Biomonitor: Examining The Interplay Between Ambient Pm10, Bulk Deposition And Heavy Metals In An Urban Environment, Scott Bradley Kiel

Dissertations and Theses

The importance of monitoring and preventing pollution in the environment is a globally recognized issue. Of the criteria pollutants outlined by the United States Environmental Protection Agency, particulate matter is among these least understood in relation to toxicity and most wide-spread. Many governments have employed continuous air quality monitoring networks to track ambient levels of particulate matter, but are often too widespread to capture the heterogeneity of the urban environment, especially for heavy metal deposition. In Portland, OR, the epiphytic moss Orthotrichum lyellii was successfully used as a low-cost passive biomonitor to increase the spatial resolution of pollution around the …


Developing A Miniature Smart Boat For Marine Research, Michael Isaac Eirinberg Jun 2022

Developing A Miniature Smart Boat For Marine Research, Michael Isaac Eirinberg

Computer Engineering

This project examines the development of a smart boat which could serve as a possible marine research apparatus. The smart boat consists of a miniature vessel containing a low-cost microcontroller to live stream a camera feed, GPS telemetry, and compass data through its own WiFi access point. The smart boat also has the potential for autonomous navigation. My project captivated the interest of several members of California Polytechnic State University, San Luis Obispo’s (Cal Poly SLO) Marine Science Department faculty, who proposed a variety of fascinating and valuable smart boat applications.


Cover Crops And Irrigation Impacts On Corn And Soybean Production In The Mid-Southern Usa, Dillon Aaron Russell May 2022

Cover Crops And Irrigation Impacts On Corn And Soybean Production In The Mid-Southern Usa, Dillon Aaron Russell

Theses and Dissertations

Reducing groundwater withdrawals from the Mississippi River Valley Alluvial Aquifer is imperative to sustain future irrigated cropping systems in the mid-southern USA. This research was conducted to determine the impacts of cover crops and irrigation sensor thresholds on corn (Zea mays L.) and soybean (Glycine max L.) production, water productivity, irrigation water use efficiency, and soil physical properties in the Mississippi Delta. The cover crop treatments included cereal rye (Secale cereale L.), hairy vetch (Vicia villosa R.), wheat (Triticum aestivum L.)-radish (Raphanus sativus L.)-turnip (Brassica rapa L.) mix, and no cover crop. The irrigation thresholds included -40 kPa, -90 kPa, …


Potato Processing In The Home: Sustainability And Nutritional Impacts, Aubin Payne May 2022

Potato Processing In The Home: Sustainability And Nutritional Impacts, Aubin Payne

Biological and Agricultural Engineering Undergraduate Honors Theses

Potatoes are the world's fourth most consumed crop. Their versatility and long shelf-life make them a staple food for millions of people worldwide. Still, the increasing consumption of highly processed potato products in developed countries has damaged the public's appreciation of potatoes as a valuable source of essential nutrients. Additionally, as public awareness of environmental sustainability increases, the average consumer is more likely to value processing methods that mitigate environmental damage.

Researchers simulated several home storage and processing conditions to find out how nutrition and environmental impact are affected by the home processing timeline. Then, a nutrient analysis, life cycle …


Root Phenotyping Of Peptide-Treated Glycine Max, Salem Jackson May 2022

Root Phenotyping Of Peptide-Treated Glycine Max, Salem Jackson

Biological Sciences Undergraduate Honors Theses

Plant elicitor peptides (Peps) – endogenous chains of amino acids involved in natural plant defense – have been shown to decrease damage from herbivores and pathogens by inducing an immune response, increasing the emission of volatile organic compounds (VOCS), transcripts, and metabolites. Exogenous treatment of soybean seeds with plant elicitor peptide GmPep3 has been shown to induce these broad-spectrum defenses and offers a new method for increasing crop yield. However, the effects of GmPep3 on indicators of soybean health – root characteristics, growth stages, etc. – have not been fully realized.

Using the root-phenotyping platform RhizoVision Explorer, several root traits …


Investigation Of The Binding Domain Interfaces Of The C-Terminus Of The Albino3 Insertase And The 43kda Chloroplast Signal Recognition Particle Subunit Via Single Molecule Förster Resonance Energy Transfer, Amanda Tomanek May 2022

Investigation Of The Binding Domain Interfaces Of The C-Terminus Of The Albino3 Insertase And The 43kda Chloroplast Signal Recognition Particle Subunit Via Single Molecule Förster Resonance Energy Transfer, Amanda Tomanek

Chemistry & Biochemistry Undergraduate Honors Theses

Fluorescent labeling is a technique used for visualizing functional groups contained in biomolecules by fluorescence imaging. This technique was used in this project to analyze post-translational targeting of light-harvesting chlorophyll-binding proteins (LHCP), which are the core complexes that harvest sunlight to drive photosynthetic electron transfer. This protein is synthesized in the cytosol and post-translationally targeted to the stroma of chloroplasts. CpSRP43 is a signal recognition particle (SRP) subunit unique to chloroplasts, which has been shown to interact with the stroma-soluble C-terminus of the thylakoid-bound Albino3 insertase (Alb3-Cterm). In the chloroplast stroma, targeting to thylakoids is performed via the cpSRP pathway …