Physical Sciences and Mathematics Commons^™

Exploring The Potential For B12-Based Oral Vaccine Delivery; Cubilin Mediated Uptake Of B12-Conjugate Systems Greater Than 160 Kda, Teresa Soldner

Honors Capstone Projects - All

This project aimed to create a novel oral tetanus vaccine by exploiting the B₁₂ uptake pathway. While many linkers were attempted, the only successful complex was created using CDT coupling. After synthesis, purification (with some difficulty), and characterization, tests were done in vitro and in vivo. These supported the conclusion that while the complex was being successfully taken up by the cell, the complex was not created a strong enough immune response for a positive titer. On a second note, simply the uptake of B₁₂-TT is remarkable because it is the largest molecule that has been …