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Full-Text Articles in Physical Sciences and Mathematics
Proteo-Lipobeads For The Oriented Encapsulation Of Membrane Proteins, Leslie M. Loew
Proteo-Lipobeads For The Oriented Encapsulation Of Membrane Proteins, Leslie M. Loew
UCHC Articles - Research
As a surrogate of the life cell, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on life cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.
Coordination Of Peroxide To The Cum Center Of Peptidylglycine Α-Hydroxylating Monooxygenase (Phm): Structural And Computational Study, Betty A. Eipper, Richard E. Mains
Coordination Of Peroxide To The Cum Center Of Peptidylglycine Α-Hydroxylating Monooxygenase (Phm): Structural And Computational Study, Betty A. Eipper, Richard E. Mains
UCHC Articles - Research
Many bioactive peptides, such as hormones and neuropeptides, require amidation at the C terminus for their full biological activity. Peptidylglycine α-hydroxylating monooxygenase (PHM) performs the first step of the amidation reaction—the hydroxylation of peptidylglycine substrates at the Cα position of the terminal glycine. The hydroxylation reaction is copper- and O2-dependent and requires 2 equiv of exogenous reductant. The proposed mechanism suggests that O2 is reduced by two electrons, each provided by one of two nonequivalent copper sites in PHM (CuH and CuM). The characteristics of the reduced oxygen species in the PHM reaction and …