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Full-Text Articles in Physical Sciences and Mathematics
Proteo-Lipobeads For The Oriented Encapsulation Of Membrane Proteins, Leslie M. Loew
Proteo-Lipobeads For The Oriented Encapsulation Of Membrane Proteins, Leslie M. Loew
UCHC Articles - Research
As a surrogate of the life cell, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on life cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.
Coordination Of Peroxide To The Cum Center Of Peptidylglycine Α-Hydroxylating Monooxygenase (Phm): Structural And Computational Study, Betty A. Eipper, Richard E. Mains
Coordination Of Peroxide To The Cum Center Of Peptidylglycine Α-Hydroxylating Monooxygenase (Phm): Structural And Computational Study, Betty A. Eipper, Richard E. Mains
UCHC Articles - Research
Many bioactive peptides, such as hormones and neuropeptides, require amidation at the C terminus for their full biological activity. Peptidylglycine α-hydroxylating monooxygenase (PHM) performs the first step of the amidation reaction—the hydroxylation of peptidylglycine substrates at the Cα position of the terminal glycine. The hydroxylation reaction is copper- and O2-dependent and requires 2 equiv of exogenous reductant. The proposed mechanism suggests that O2 is reduced by two electrons, each provided by one of two nonequivalent copper sites in PHM (CuH and CuM). The characteristics of the reduced oxygen species in the PHM reaction and …
Long Distance Electron Transfer Across >100 Nm Thick Au Nanoparticle/Polyion Films To A Surface Redox Protein, James F. Rusling, Hongyun Liu, Yasemin Kutes, Bryan D. Huey
Long Distance Electron Transfer Across >100 Nm Thick Au Nanoparticle/Polyion Films To A Surface Redox Protein, James F. Rusling, Hongyun Liu, Yasemin Kutes, Bryan D. Huey
UCHC Articles - Research
Glutathione-decorated 5 nm gold nanoparticles (AuNPs) and oppositely charged poly(allylamine hydrochloride) (PAH) were assembled into {PAH/AuNP}n films fabricated layer-by-layer (LbL) on pyrolytic graphite (PG) electrodes. These AuNP/polyion films utilized the AuNPs as electron hopping relays to achieve direct electron transfer between underlying electrodes and redox proteins on the outer film surface across unprecedented distances >100 nm for the first time. As film thickness increased, voltammetric peak currents for surface myoglobin (Mb) on these films decreased but the electron transfer rate was relatively constant, consistent with a AuNP-mediated electron hopping mechanism.
Control Of Electrochemical And Ferryloxy Formation Kinetics Of Cyt P450s In Polyion Films By Heme Iron Spin State And Secondary Structure, Sadagopan Krishnan, Amila Abeykoon, John B. Schenkman, James F. Rusling
Control Of Electrochemical And Ferryloxy Formation Kinetics Of Cyt P450s In Polyion Films By Heme Iron Spin State And Secondary Structure, Sadagopan Krishnan, Amila Abeykoon, John B. Schenkman, James F. Rusling
UCHC Articles - Research
Voltammetry of cytochrome P450 (cyt P450) enzymes in ultrathin films with polyions was related for the first time to electronic and secondary structure. Heterogeneous electron transfer (hET) rate constants for reduction of the cyt P450s depended on heme iron spin state, with low spin cyt P450cam giving a value 40-fold larger than high spin human cyt P450 1A2, with mixed spin human P450 cyt 2E1 at an intermediate value. Asymmetric reduction–oxidation peak separations with increasing scan rates were explained by simulations featuring faster oxidation than reduction. Results are consistent with a square scheme in which oxidized and reduced forms of …
Retention Of Polarization Signatures In Shg Microscopy Of Scattering Tissues Through Optical Clearing, Oleg Nadiarnykh, Paul J. Campagnola
Retention Of Polarization Signatures In Shg Microscopy Of Scattering Tissues Through Optical Clearing, Oleg Nadiarnykh, Paul J. Campagnola
UCHC Articles - Research
Polarization responses in Second Harmonic Generation (SHG) imaging microscopy are a valuable method to quantify aspects of tissue structure, and may be a means to differentiate normal and diseased tissues. Due to multiple scattering, the polarization data is lost in turbid tissues. Here we investigate if this information can be retained through the use of optical clearing which greatly reduces the scattering coefficient and increases the corresponding mean free path. To this end, we have measured the SHG intensity as a function of laser polarization and the SHG signal anisotropy in murine tendon and striated muscle over a depth range …