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Articles 1 - 9 of 9
Full-Text Articles in Physical Sciences and Mathematics
Role Of Surface Charge Density In Nanoparticle-Templated Assembly Of Bromovirus Protein Cages, Mc Daniel, Ib Tsvetkova, Zt Quinkert, A Murali, M De, Vm Rotello, Cc Kao, B Dragnea
Role Of Surface Charge Density In Nanoparticle-Templated Assembly Of Bromovirus Protein Cages, Mc Daniel, Ib Tsvetkova, Zt Quinkert, A Murali, M De, Vm Rotello, Cc Kao, B Dragnea
Vincent Rotello
Self-assembling icosahedral protein cages have potencially useful physical and chemical characteristics for a variety of nanotechnology applications, ranging from therapeutic or diagnostic vectors to building blocks for hierarchical materials. For application-specific functional control of protein cage assemblies, a deeper understanding of the interaction between the protein cage and its payload is necessary. Protein-cage encapsulated nanoparticles, with their well-defined surface chemistry, allow for systematic control over key parameters of encapsulation such as the surface charge, hydrophobicity, and size. Independent control over these variables allows experimental testing of different assembly mechanism models. Previous studies done with Brome mosaic virus capsids and negatively …
Effect Of Nanoparticle Surface Charge At The Plasma Membrane And Beyond, Rr Arvizo, Or Miranda, Ma Thompson, Cm Pabelick, R Bhattacharya, Jd Robertson, Vm Rotello, Ys Prakash, P Mukherjee
Effect Of Nanoparticle Surface Charge At The Plasma Membrane And Beyond, Rr Arvizo, Or Miranda, Ma Thompson, Cm Pabelick, R Bhattacharya, Jd Robertson, Vm Rotello, Ys Prakash, P Mukherjee
Vincent Rotello
Herein, we demonstrate that the surface charge of gold nanoparticles (AuNPs) plays a critical role in modulating membrane potential of different malignant and nonmalignant cell types and subsequent downstream intracellular events. The findings presented here describe a novel mechanism for cell-nanoparticle interactions and AuNP uptake: modulation of membrane potential and its effect on intracellular events. These studies will help understand the biology of cell-nanoparticle interactions and facilitate the engineering of nanoparticles for specific intracellular targets.
Gold Nanoparticle Self-Assembly Promoted By A Non-Covalent, Charge-Complemented Coiled-Coil Peptide, D Ernenwein, P Ghosh, V Rotello, J Chmielewski
Gold Nanoparticle Self-Assembly Promoted By A Non-Covalent, Charge-Complemented Coiled-Coil Peptide, D Ernenwein, P Ghosh, V Rotello, J Chmielewski
Vincent Rotello
Non-covalent interactions between cationic gold nanoparticles and an anionic coiled-coil peptide were harnessed for coiled-coil mediated self-assembly of gold nanoparticles (GNPs)
Enzyme-Amplified Array Sensing Of Proteins In Solution And In Biofluids, Or Miranda, Ht Chen, Cc You, De Mortenson, Xc Yang, Uhf Bunz, Vm Rotello
Enzyme-Amplified Array Sensing Of Proteins In Solution And In Biofluids, Or Miranda, Ht Chen, Cc You, De Mortenson, Xc Yang, Uhf Bunz, Vm Rotello
Vincent Rotello
We have developed an enzyme−nanoparticle sensor array where the sensitivity is amplified through enzymatic catalysis. In this approach cationic gold nanoparticles are electrostatically bound to an enzyme (β-galactosidase, β-Gal), inhibiting enzyme activity. Analyte proteins release the β-Gal, restoring activity and providing an amplified readout of the binding event. Using this strategy we have been able to identify proteins in buffer at a concentration of 1 nM, substantially lower than current strategies for array-based protein sensing. Moreover, we have obtained identical sensitivity in studies where the proteins are spiked into the complex protein matrix provided by desalted human urine (1.5 μM …
Recognition Mediated Encapsulation And Isolation Of Flavin-Polymer Conjugates Using Dendritic Guest Moieties, C Subramani, G Yesilbag, Bj Jordan, Xn Li, A Khorasani, G Cooke, A Sanyal, Vm Rotello
Recognition Mediated Encapsulation And Isolation Of Flavin-Polymer Conjugates Using Dendritic Guest Moieties, C Subramani, G Yesilbag, Bj Jordan, Xn Li, A Khorasani, G Cooke, A Sanyal, Vm Rotello
Vincent Rotello
Diaminopyridine dendritic scaffolds encapsulate polymeric flavin via non-covalent interactions and demonstrate isolation of the redox moiety.
Chemically Directed Immobilization Of Nanoparticles Onto Gold Substrates For Orthogonal Assembly Using Dithiocarbamate Bond Formation, Mh Park, Xx Duan, Y Ofir, B Creran, D Patra, Xy Ling, J Huskens, Vm Rotello
Chemically Directed Immobilization Of Nanoparticles Onto Gold Substrates For Orthogonal Assembly Using Dithiocarbamate Bond Formation, Mh Park, Xx Duan, Y Ofir, B Creran, D Patra, Xy Ling, J Huskens, Vm Rotello
Vincent Rotello
Dithiocarbamate-mediated bond formation combined with soft lithography was used for the selective immobilization of amine-functionalized silica nanoparticles on gold substrates. The available amine groups on the upper surface of the immobilized silica nanoparticles were further utilized for postdeposition of additional materials including particles, dyes, and biomolecules. The robustness of dithiocarbamate-mediated immobilization enables orthogonal assembly on surfaces via selective removal of the masking thiol ligands using iodine vapor etching followed by further functionalization.
Hyperbranched Conjugated Polymers: Postfunctionalization, C Kub, J Tolosa, Aj Zucchero, Pl Mcgrier, C Subramani, A Khorasani, Vm Rotello, Uhf Bunz
Hyperbranched Conjugated Polymers: Postfunctionalization, C Kub, J Tolosa, Aj Zucchero, Pl Mcgrier, C Subramani, A Khorasani, Vm Rotello, Uhf Bunz
Vincent Rotello
Out on a limb: Sonogashira coupling of a suitable AB(2) monomer containing two iodine and one alkyne group forms a hyperbranched conjugated polymer that is studded with iodine end groups (see picture: I purple). These iodine groups are a perfect handle for convenient, efficient, and high-yielding post-functionalization to access hyperbranched, fluorescent poly(phenyleneethynylene)s.
Intracellular Delivery Of A Membrane-Impermeable Enzyme In Active Form Using Functionalized Gold Nanoparticles, P Ghosh, Xc Yang, R Arvizo, Zj Zhu, Ss Agasti, Zh Mo, Vm Rotello
Intracellular Delivery Of A Membrane-Impermeable Enzyme In Active Form Using Functionalized Gold Nanoparticles, P Ghosh, Xc Yang, R Arvizo, Zj Zhu, Ss Agasti, Zh Mo, Vm Rotello
Vincent Rotello
Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.
Array-Based Sensing Of Normal, Cancerous, And Metastatic Cells Using Conjugated Fluorescent Polymers, A Bajaj, Or Miranda, R Phillips, Ib Kim, Dj Jerry, Uhf Bunz, Vm Rotello
Array-Based Sensing Of Normal, Cancerous, And Metastatic Cells Using Conjugated Fluorescent Polymers, A Bajaj, Or Miranda, R Phillips, Ib Kim, Dj Jerry, Uhf Bunz, Vm Rotello
Vincent Rotello
A family of conjugated fluorescent polymers was used to create an array for cell sensing. Fluorescent conjugated polymers with pendant charged residues provided multivalent interactions with cell membranes, allowing the detection of subtle differences between different cell types on the basis of cell surface features. Highly reproducible characteristic patterns were obtained from different cell types as well as from isogenic cell lines, enabling the identification of the cell type as well differentiating between normal, cancerous, and metastatic isogenic cell types with high accuracy.