Translational Medical Research Commons^™

Pioglitazone Induces Apoptosis Of Macrophages In Human Adipose Tissue, Angela M. Bodles, Vijayalakshmi Varma, Aiwei Yao-Borengasser, Bounleut Phanavanh, Charlotte A. Peterson, Robert E. Mcgehee Jr., Neda Rasouli, Martin Wabitsch, Philip A. Kern

Clinical and Translational Science Faculty Publications

Metabolic syndrome and type 2 diabetes mellitus are associated with an increased number of macrophage cells that infiltrate white adipose tissue (WAT). Previously, we demonstrated that the treatment of subjects with impaired glucose tolerance (IGT) with the peroxisome proliferator-activated receptor γ (PPARγ) agonist pioglitazone resulted in a decrease in macrophage number in adipose tissue. Here, adipose tissue samples from IGT subjects treated with pioglitazone were examined for apoptosis with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining. TUNEL-positive cells were identified, and there was a significant 42% increase in TUNEL-positive cells following pioglitazone treatment. Overlay experiments with anti-CD68 antibody …