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Full-Text Articles in Other Medicine and Health Sciences
Accelerated Clearing And Molecular Labeling Of Biological Tissues Using, Joseph Dwyer, M Desmond Ramirez, Paul S. Katz, Rolf O. Karlstrom, Joseph Bergan
Accelerated Clearing And Molecular Labeling Of Biological Tissues Using, Joseph Dwyer, M Desmond Ramirez, Paul S. Katz, Rolf O. Karlstrom, Joseph Bergan
Psychological and Brain Sciences Faculty Publication Series
Techniques used to clear biological tissue for fluorescence microscopy are essential to connect anatomical principles at levels ranging from subcellular to the whole animal. Here we report a simple and straightforward approach to efficiently render opaque tissue samples transparent and show that this approach can be modified to rapidly label intact tissue samples with antibodies for large volume fluorescence microscopy. This strategy applies a magnetohydrodynamic (MHD) force to accelerate the removal of lipids from tissue samples at least as large as an intact adult mouse brain. We also show that MHD force can be used to accelerate antibody penetration into …
Mothers' Sleep Deficits And Cognitive Performance: Moderation By Stress And Age, Kirby Deater-Deckard, Mamatha Chary, Maureen E. Mcquillan, Angela D. Staples, John E. Bates
Mothers' Sleep Deficits And Cognitive Performance: Moderation By Stress And Age, Kirby Deater-Deckard, Mamatha Chary, Maureen E. Mcquillan, Angela D. Staples, John E. Bates
Psychological and Brain Sciences Faculty Publication Series
There are well-known associations between stress, poor sleep, and cognitive deficits, but little is known about their interactive effects, which the present study explored in a sample of mothers of toddlers. Since certain types of cognitive decline start during the 20s and continue into later ages, we also explored whether mothers' age interacted with stress and sleep in the prediction of cognitive functioning. We hypothesized that poorer sleep [measured using one week of 24-hour wrist actigraphy data] and having more chronic stressors [e.g., life events, household chaos, work/family role conflict] would be linked with poorer cognitive performance [both executive function …