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Enhanced Detection Of Groundwater Contamination From A Leaking Waste Disposal Site By Microbial Community Profiles, Paula J. Mouser, Donna M. Rizzo, Gregory K. Druschel, Sergio E. Morales, Nancy Hayden, Patrick O'Grady, Lori Stevens Dec 2010

Enhanced Detection Of Groundwater Contamination From A Leaking Waste Disposal Site By Microbial Community Profiles, Paula J. Mouser, Donna M. Rizzo, Gregory K. Druschel, Sergio E. Morales, Nancy Hayden, Patrick O'Grady, Lori Stevens

College of Engineering and Mathematical Sciences Faculty Publications

Groundwater biogeochemistry is adversely impacted when municipal solid waste leachate, rich in nutrients and anthropogenic compounds, percolates into the subsurface from leaking landfills. Detecting leachate contamination using statistical techniques is challenging because well strategies or analytical techniques may be insufficient for detecting low levels of groundwater contamination. We sampled profiles of the microbial community from monitoring wells surrounding a leaking landfill using terminal restriction fragment length polymorphism (T-RFLP) targeting the 16S rRNA gene. Results show in situ monitoring of bacteria, archaea, and the family Geobacteraceae improves characterization of groundwater quality. Bacterial T-RFLP profiles showed shifts correlated to known gradients of …


Conclusions About Niche Expansion In Introduced Impatiens Walleriana Populations Depend On Method Of Analysis, Lisa Mandle, Dan L. Warren, Matthias H. Hoffmann, A. Townsend Peterson, Johanna Schmitt, Eric J. Von Wettberg Dec 2010

Conclusions About Niche Expansion In Introduced Impatiens Walleriana Populations Depend On Method Of Analysis, Lisa Mandle, Dan L. Warren, Matthias H. Hoffmann, A. Townsend Peterson, Johanna Schmitt, Eric J. Von Wettberg

College of Agriculture and Life Sciences Faculty Publications

Determining the degree to which climate niches are conserved across plant species' native and introduced ranges is valuable to developing successful strategies to limit the introduction and spread of invasive plants, and also has important ecological and evolutionary implications. Here, we test whether climate niches differ between native and introduced populations of Impatiens walleriana, globally one of the most popular horticultural species. We use approaches based on both raw climate data associated with occurrence points and ecological niche models (ENMs) developed with Maxent. We include comparisons of climate niche breadth in both geographic and environmental spaces, taking into account differences …


Nutrient Enrichment Enhances Hidden Differences In Phenotype To Drive A Cryptic Plant Invasion, Christine Holdredge, Mark D. Bertness, Eric Von Wettberg, Brian R. Silliman Nov 2010

Nutrient Enrichment Enhances Hidden Differences In Phenotype To Drive A Cryptic Plant Invasion, Christine Holdredge, Mark D. Bertness, Eric Von Wettberg, Brian R. Silliman

College of Agriculture and Life Sciences Faculty Publications

Many mechanisms of invasive species success have been elucidated, but those driving cryptic invasions of non-native genotypes remain least understood. In one of the most successful cryptic plant invasions in North America, we investigate the mechanisms underlying the displacement of native Phragmites australis by its Eurasian counterpart. Since invasive Phragmites' populations have been especially prolific along eutrophic shorelines, we conducted a two-year field experiment involving native and invasive genotypes that manipulated nutrient level and competitor identity (inter- and intra-genotypic competition) to assess their relative importance in driving the loss of native Phragmites. Inter-genotypic competition suppressed aboveground biomass of both native …


Protecting Degraded Rainforests: Enhancement Of Forest Carbon Stocks Under Redd+, David P. Edwards, Brendan Fisher, Emily Boyd Sep 2010

Protecting Degraded Rainforests: Enhancement Of Forest Carbon Stocks Under Redd+, David P. Edwards, Brendan Fisher, Emily Boyd

Rubenstein School of Environment and Natural Resources Faculty Publications

The likely Reducing Emissions from Deforestation and Degradation (REDD+) mechanism includes strategies for the enhancement of forest carbon stocks. Recent concerns have been expressed that such enhancement, or restoration, of forest carbon could be counterproductive to biodiversity conservation, because forests are managed as "carbon farms" with the application of intensive silvicultural management that could homogenize diverse degraded rainforests. Restoration increases regeneration rates in degraded forest compared to naturally regenerating forest, and thus could yield significant financial returns for carbon sequestered. Here, we argue that such forest restoration projects are, in fact, likely to provide a number of benefits to biodiversity …


How Anthocyanin Mutants Respond To Stress: The Need To Distinguish Between Stress Tolerance And Maximal Vigour, Eric J. Von Wettberg, Maureen L. Stanton, Justen B. Whittall May 2010

How Anthocyanin Mutants Respond To Stress: The Need To Distinguish Between Stress Tolerance And Maximal Vigour, Eric J. Von Wettberg, Maureen L. Stanton, Justen B. Whittall

College of Agriculture and Life Sciences Faculty Publications

Background: Anthocyanins are produced by plants in response to diverse stresses. Mutants that block the anthocyanin biosynthetic pathway (ABP) at various steps can easily be compared across numerous abiotic stresses. Hypothesis: Anthocyanins or their precursors are required for stress tolerance. Thus, ABP loss-of-function mutants should have proportionately lower fitness than wildtype plants under stress, compared with benign conditions. In contrast, a decrease in maximal vigour - the general capacity for growth and fecundity - should be most pronounced under benign conditions that allow luxuriant growth by the most vigorous genotypes. Tests: Determine whether, under stressful conditions, ABP loss-of-function mutants have …


Fire Effects On Demography Of The Invasive Shrub Brazilian Pepper (Schinus Terebinthifolius) In Florida Pine Savannas, Jens T. Stevens, Brian Beckage Jan 2010

Fire Effects On Demography Of The Invasive Shrub Brazilian Pepper (Schinus Terebinthifolius) In Florida Pine Savannas, Jens T. Stevens, Brian Beckage

College of Agriculture and Life Sciences Faculty Publications

Fire is a common disturbance in savanna ecosystems that may either facilitate or impede non-native plant invasions. Although fire can create recruitment opportunities for non-native plants, it can also prevent their invasion if it exerts strong negative effects on their demographic processes. Some savannas may, therefore, be able to resist invasion provided the natural, frequent-fire regime remains intact. We examined the effects of fire on the demography of the invasive shrub Brazilian pepper, Schinus terebinthifolius Raddi., which is invading fire-prone slash pine savannas of southern Florida. We studied survivorship, growth, and reproduction of low-density populations of Brazilian pepper in a …