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Full-Text Articles in Congenital, Hereditary, and Neonatal Diseases and Abnormalities
The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation By Hsp-90, Kevin Marrs
The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation By Hsp-90, Kevin Marrs
Theses and Dissertations (ETD)
The hypothesis of this project is that a functionally relevant association exists between CFTR and Hsp90. In testing this hypothesis, (a) the association of CFTR and Hsp90 is confirmed via crosslinking and immunoprecipitation of CFTR from stably transfected HEK-293 cells, electrophoretic separation of cross-linked proteins, and protein identification via SDS-PAGE, western blotting, and mass spectrometry. Also, this hypothesis is tested by (b) functionally assessing the effect of Hsp90 on CFTR function via Ussing chamber analysis, two-electrode voltage clamp analysis, preliminary in vivoanimal studies, and protein phosphorylation. The HEK-293 Flag-CFTR cells used in this study allows for a high degree …