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Effects Of Ascorbic Acid On Impaired Vascular Reactivity In Aortas Isolated From Age-Matched Hypertensive And Diabetic Rats, Mohd Rais Mustafa, Macha Ajay
Effects Of Ascorbic Acid On Impaired Vascular Reactivity In Aortas Isolated From Age-Matched Hypertensive And Diabetic Rats, Mohd Rais Mustafa, Macha Ajay
Mohd Rais Mustafa
Impaired vascular reactivity is a hallmark of several cardiovascular diseases that include hypertension and diabetes. This study compared the changes in vascular reactivity in age-matched experimental hypertension and diabetes, and, subsequently, tested whether these changes could be affected directly by ascorbic acid (10 microM). Endothelium-derived nitric oxide (NO) modulation of ascorbic acid effects was also investigated. All the experiments were performed in the presence of a cyclooxygenase inhibitor, indomethacin (10 microM). Results showed that the endothelium-dependent and -independent relaxations induced by acetylcholine (ACh) and sodium nitroprusside (SNP), respectively, were blunted to a similar extent in isolated aortic rings from age-matched …
Bioassay-Guided Isolation Of A Vasorelaxant Active Compound From Kaempferia Galanga L, Mohd Rais Mustafa, Khalijah Awang, Mustafa Ali Mohd, Rozana Othman, Halijah Ibrahim
Bioassay-Guided Isolation Of A Vasorelaxant Active Compound From Kaempferia Galanga L, Mohd Rais Mustafa, Khalijah Awang, Mustafa Ali Mohd, Rozana Othman, Halijah Ibrahim
Mohd Rais Mustafa
Bioassay-guided fractionation was performed on a crude dichloromethane extract of Kaempferia galanga L. using chromatography techniques. Screening of the extract for biological activity started with the brine shrimp lethality bioassay, followed by the study of its antihypertensive activity on anaesthetized rats, which involved monitoring of the extract's effect on mean arterial blood pressure. The components of the fractions obtained from the separation procedures were analyzed using gas chromatography (GC). The yield of the CH2Cl2 extract was 0.29% of the crude plant extract. Analysis of the data for brine shrimp lethality test using the Finney computer program showed that this extract …