Chemicals and Drugs Commons^™

Real-Time In Vivo Detection Of Biomaterial-Induced Reactive Oxygen Species, Minglin Ma, Wendy F. Liu, Kaitlin M. Bratlie, Tram Dang, Robert Langer, Daniel G. Anderson

Kaitlin M. Bratlie

The non-specific host response to implanted biomaterials is often a key challenge of medical device design. To evaluate biocompatibility, measuring the release of reactive oxygen species (ROS) produced by inflammatory cells in response to biomaterial surfaces is a well-established method. However, the detection of ROS in response to materials implanted in vivo has not yet been demonstrated. Here, we develop a bioluminescence whole animal imaging approach to observe ROS released in response to subcutaneously-implanted materials in live animals. We compared the real-time generation of ROS in response to two representative materials, polystyrene and alginate, over the course of 28 days. …