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Effects Of Arginine On The Kinetics Of Bovine Insulin Aggregation Studied By Dynamic Light Scattering, Michael M. Varughese

Honors Theses

In the fields of protein science and medicine, understanding the kinetics of protein aggregation are significant in the research and treatment of certain amyloid diseases such as Alzheimer’s. Previous studies have suggested that arginine can increase the solubility of certain proteins, suppress protein aggregation, and assist in the refolding of aggregated proteins; however, the molecular mechanisms by which arginine can influence protein aggregation are still unclear. Bovine insulin was employed as a model system for further understanding the effects of arginine on protein aggregation. Using Dynamic Light Scattering (DLS), we studied the concentration-dependent and temperature-dependent suppression of aggregation in insulin …