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Full-Text Articles in Anatomy
Enumeration Of Endothelial Progenitor Cells In Hind Limb Suspended Rats And The Mechanics Of Endothelial Wound Healing, Jarrod Matthew Pennington
Enumeration Of Endothelial Progenitor Cells In Hind Limb Suspended Rats And The Mechanics Of Endothelial Wound Healing, Jarrod Matthew Pennington
Theses, Dissertations and Capstones
The endothelium repairs itself through two methods. One is by the activity of circulating endothelial progenitor cells (EPCs). EPCs are immature endothelial cells that circulate the bloodstream that are capable of proliferation and differentiation into mature endothelium. It is thought that EPCs contribute to the repair and replacement of damaged endothelial cells in the process of reendothelialization. Physical inactivity and bed rest are known to be deleterious to the endothelium. It is possible that this inactivity is also deleterious to the number of viable EPCs, which would reduce the ability of the endothelium to repair itself. We used hind limb …
The Expression And Function Of Ppar And Hif-1 In Human Melanoma, Caroline Mills
The Expression And Function Of Ppar And Hif-1 In Human Melanoma, Caroline Mills
Theses, Dissertations and Capstones
The first part of my dissertation focuses on the expression and function of PPARs in human melanoma. I found that the A375 cells were significantly growth inhibited in response to PGJ2 and troglitazone treatment. HEMn-LP showed significant growth inhibition in response to troglitazone. I found that PPARγ and PPARδ mRNA is present in both the SK-Mel 28 and A375 cells. The relative level of PPARα mRNA expression is highest in SK-Mel 28 cells, ~3 fold higher relative to both the normal human melanocytes and A375 cells. PPARγ protein was ~50% higher in both SK-Mel 28 and A375 cells relative to …
The Regulation Of Manganese Superoxide Dismutase And Inducible Nitric Oxide Synthase By Nuclear Factor-Kappa B And Activator Protein 1 In Rat Aortic Endothelial Cells, Scott L. Cobbs
Theses, Dissertations and Capstones
Inducible nitric oxide synthase (iNOS) and manganese superoxide dismutase (MnSOD) are two enzymes that influence reactive oxygen species within the cell. The promoter regions for the genes encoding these enzymes have two specific transcription factor elements in common, activator protein 1 (AP-1) and nuclear factor kappa b (NF-KB). This study was initiated to determine if either NF-KB or AP-1 regulates the transcription of both genes in endothelial cells. To test whether NF-KB or AP-1 binding sites in the MnSOD and iNOS promoter region were important for transcriptional regulation, we performed induction analysis using various deletion constructs of MnSOD and iNOS …