Enamel Matrix Derivative Has No Effect On The Chondrogenic Differentiation Of Mesenchymal Stem Cells, 2014 Royal College of Surgeons in Ireland
Enamel Matrix Derivative Has No Effect On The Chondrogenic Differentiation Of Mesenchymal Stem Cells, Lisanne C. Groeneveldt, Callie Knuth, Janneke Witte-Bourna, Fergal O'Brien, Eppo B. Wolvius, Eric Farrell
Background: Treatment of large bone defects due to trauma, tumor resection, or congenital abnormalities is challenging. Bone tissue engineering using mesenchymal stem cells (MSCs) represents a promising treatment option. However, the quantity and quality of engineered bone tissue are not sufficient to fill large bone defects. The aim of this study was to determine if the addition of enamel matrix derivative (EMD) improves in vitro chondrogenic priming of MSCs to ultimately improve in vivo MSC mediated endochondral bone formation.
Methods: MSCs were chondrogenically differentiated in 2.0 × 105 cell pellets in medium supplemented with TGFβ3 in the absence or presence ...
Surgical Approach In Total Hip Arthroplasty: Patient Outcomes And Impact On Costs, 2014 Western University
Surgical Approach In Total Hip Arthroplasty: Patient Outcomes And Impact On Costs, Stephen M. Petis
University of Western Ontario - Electronic Thesis and Dissertation Repository
Total hip arthroplasty is often renowned as one of the most important surgical advances of the past century. Orthopaedic surgeons must choose a surgical approach to gain access to the hip joint in order to perform the reconstruction. There is debate in the literature as to which surgical approach optimizes patient outcomes, minimizes complications, and reduces costs to hospitals as a high volume procedure.
In the current studies, patient reported outcomes were compared at short-term follow-up using a prospective study design across the anterior, posterior, and lateral approach. A micro-costing method was used to acquire costs related to each procedure ...
Vascular Architecture Of The Liver In Shr And Wky Rats, 2014 Minnesota State University, Mankato
Vascular Architecture Of The Liver In Shr And Wky Rats, Charalette Mathwig, Kenneth Willaert
Journal of Undergraduate Research at Minnesota State University, Mankato
The liver is a highly complex vascular organ containing an intricate network of arteries, arterioles, portal veins, veins, venules, and capillaries. This complex network could change in situations related to vascular disease such as hypertension. We examined the vasculature in rat livers by scanning electron microscopy. The tissue was prepared by perfusing either Mercox resin or polyurethane resin into the vasculature. Once the resin polymerized, each liver was placed in concentrated potassium hydroxide to corrode the tissue from the vascular casts. The casts were critical point dried, sputter coated with gold-palladium, and viewed by scanning electron microscopy. Examination of the ...
Simultaneous Measurement Of Smoothened Entry Into And Exit From The Primary Cilium, 2014 Himmelfarb Health Sciences Library, The George Washington University
Simultaneous Measurement Of Smoothened Entry Into And Exit From The Primary Cilium, Jynho Kim, Elaine Y.C. Hsia, James Kim, Navdar Sever, Philip A. Beachy, Xiaoyan Zheng
Anatomy and Regenerative Biology Faculty Publications
Ciliary accumulation of signaling proteins must result from a rate of ciliary entry that exceeds ciliary exit, but approaches for distinguishing ciliary entry vs. exit are lacking. Using a photoconvertible fluorescent protein tag, we establish an assay that allows a separate but simultaneous examination of ciliary entry and exit of the Hedgehog signaling protein Smoothened in individual cells. We show that KAAD-cyclopamine selectively blocks entry, whereas ciliobrevin interferes initially with exit and eventually with both entry and exit of ciliary Smoothened. Our study provides an approach to understanding regulation of ciliary entry vs. exit of Hedgehog signaling components as well ...
Pathscan Enabler At Md Anderson Cancer Center, 2014 The University of Texas
Pathscan Enabler At Md Anderson Cancer Center, George Mcnamara
McNamara 20140703 - Additional Pathscan and Tiki_Goddess related resources
Our Pathscan Enabler IV, delivered July 2014, uses the QuickScan software to scan a histology slide in "two clicks" (after loading the
1. Push the QuickScan button on the front of the Pathscan Enabler.
2. Click the Scan button in the QuickScan pop-up application (optional: change scan area in the Prescan image window).
The image gets saved as a TIFF file to ...
Interaction Among Gustation, Olfaction, And Vision In Flavor Identification, 2014 California Polytechnic State University
Interaction Among Gustation, Olfaction, And Vision In Flavor Identification, Michael J. Lauth
Even though the senses of taste, smell, and sight are distinct, there is a significant overlap among them in our perceptions of objects that helps us understand and differentiate the world. Everyone has experienced, when his or her nose gets congested, that his or her sense of taste changes as well. Many individuals do not equally understand the top-down processing with taste when someone sees objects they are about to eat. In the replicated study by our principle investigator, a random convenience sample of young adults (n=162) were recruited and tested to determine if they could taste four Jelly ...
Dreams And Learning, 2014 California Polytechnic State University
Dreams And Learning, Cody Mebane Gibbons
Project Summary: This study is being done to test one aspect of how paying attention to one’s dreams may influence our waking lives. The idea was inspired by research linking the brain processes involved in long-term memory storage to qualities of dreaming, as well as the potential for learning in lucid dreams. It is hypothesized that the more conscious one is of one’s dreams and dream world, the better one will be at learning. In order to test this, the dreaming ability of 300 Cal Poly students will be analyzed via dream questionnaires with the purpose of seeing ...
Activation Of C-Met And Upregulation Of Cd44 Expression Are Associated With The Metastatic Phenotype In The Colorectal Cancer Liver Metastasis Model, Victoria Allison Elliott, Piotr G. Rychahou, Yekaterina Y. Zaytseva, B. Mark Evers
Markey Cancer Center Faculty Publications
Liver metastasis is the most common cause of death in patients with colorectal cancer. Despite extensive research into the biology of cancer progression, the molecular mechanisms that drive colorectal cancer metastasis are not well characterized.
HT29 LM1, HT29 LM2, HT29 LM3 cell lines were derived from the human colorectal cancer cell line HT29 following multiple rounds of in vivo selection in immunodeficient mice.
CD44 expression, a transmembrane glycoprotein involved in cell-cell and cell-matrix adhesions, and cancer cells adhesion to endothelial cells was increased in all in vivo selected cell lines, with maximum CD44 expression and cancer cells ...
A Comparison Of Angiography Versus Intravascular Ultrasound In The Treatment Of Peripheral Arterial Disease, 2014 University of South Carolina
A Comparison Of Angiography Versus Intravascular Ultrasound In The Treatment Of Peripheral Arterial Disease, Michael Pompliano
Peripheral Arterial Disease is a growing epidemic throughout the United States. It is estimated that 8 to 12 million Americans currently suffer from PAD, a disease of the circulatory system that limits blood flow to your hands and feet. This limited blood flow is due to the narrowing of the arteries that supply blood throughout your body and can disrupt the balance of the nerves and tissues that make up your extremities. If left untreated, it can cause irreparable, life- threatening damage that may result in amputation of the diseased limb. Although the mechanism of PAD is known and well ...
"What Happens In Romania..." Comes Back To The United States And Becomes A Quilt, 2014 University of South Carolina
"What Happens In Romania..." Comes Back To The United States And Becomes A Quilt, Ashley Ehlers, Kaitlyn Torres
Our senior thesis project is a quilt that chronicles our experiences on the Maymester trip to Romania through the Honors College, during which we were able to shadow surgeons in the Oncology Hospital and General Surgery III Hospital in Cluj-Napoca. In our quilt, we included some of the most common surgeries we saw while shadowing Romanian doctors: breast removal, gall bladder removal, appendix removal, removal of a section of the large intestine, and removal of the uterus. The final product of this quilt shows every level of the abdominal muscles and organs from the anterior skin to the kidneys.
Functionalization And Modification Of Naphthaquinone Analogs As Her2 Kinase Inhibitors, 2014 Western Kentucky University
Functionalization And Modification Of Naphthaquinone Analogs As Her2 Kinase Inhibitors, Divya Jyothi Lella
Masters Theses & Specialist Projects
HER2 overexpression in breast cancer tumors predicts lower overall survival. Because of the aggressive nature of HER2 tumors and the association with metastatic disease, the HER2 receptor holds great promise as a therapeutic target in metastatic breast cancer. We are developing small molecule inhibitors that bind to the ATP binding site of the tyrosine kinase domain in order to inhibit tyrosine auto-phosphorylation. This process controls biological pathways that mediate the cell growth. In normal cells this process is highly controlled. We are targeting the modification of the side chain of the hydroxy methyl group of 2-Hydroxy methyl-5,8-dimethoxy-1,4-naphthaquinone. These ...
A Biomimetic Multi-Layered Collagen-Based Scaffold For Osteochondral Repair., 2014 Royal College of Surgeons in Ireland
A Biomimetic Multi-Layered Collagen-Based Scaffold For Osteochondral Repair., Tanya J. Levingstone, Amos Matsiko, Glenn R Dickson, Fergal J. O'Brien, John P. Gleeson
Cartilage and osteochondral defects pose a significant challenge in orthopedics. Tissue engineering has shown promise as a potential method for the treatment of such defects; however, a long-lasting repair strategy has yet to be realized. This study focuses on the development of a layered construct for osteochondral repair, fabricated through a novel "iterative layering" freeze-drying technique. The process involved repeated steps of layer addition followed by freeze-drying, enabling control over material composition, pore size and substrate stiffness in each region of the construct, while also achieving a seamlessly integrated layer structure. The novel construct developed mimics the inherent gradient structure ...
Structural Alterations To The Axon Initial Segment Following Diffuse Axonal Injury As A Consequence Of Age, 2014 Virginia Commonwealth University
Structural Alterations To The Axon Initial Segment Following Diffuse Axonal Injury As A Consequence Of Age, William Behl
Theses and Dissertations
An epidemiological shift towards the elderly population has occurred in traumatic brain injury (TBI). Age is believed to be one of the strongest prognostic indicators following TBI. Diffuse axonal injury (DAI), a prevalent feature of TBI, is believed to be the primary cause for much of the morbidity and mortality associated with TBI. The pathobiology associated with DAI is believed to occur in response to the primary injury in a progressive, secondary fashion. Though the injury mechanisms behind DAI have been shown to occur at numerous sites along the axon, recent work suggests that the axon initial segment (AIS) may ...
The Osteogenic Potential Of The Marine-Derived Multi-Mineral Formula Aquamin Is Enhanced By The Presence Of Vitamin D., 2014 Royal College of Surgeons in Ireland
The Osteogenic Potential Of The Marine-Derived Multi-Mineral Formula Aquamin Is Enhanced By The Presence Of Vitamin D., Amro Widaa, Orlaith Brennan, D M. O'Gorman, Fergal J. O'Brien
Bone degenerative diseases are on the increase globally and are often problematic to treat. This has led to a demand to identify supplements that aid bone growth and formation. Aquamin is a natural multi-mineral food supplement, derived from the red algae Lithothamnion species which contains calcium, magnesium and 72 other trace minerals. It has been previously reported to increase bone formation and mineralisation. This study aimed to investigate the 28 day in vitro osteogenic response of Aquamin supplemented with Vitamin D. The osteogenic potential of MC3T3-E1 osteoblast-like cells was analysed in standard osteogenic medium supplemented with Aquamin +/- Vitamin D3, and ...
Assessing The Relationship Between The Blood-Air Partition Coefficient And Fractional Uptake Of Inspired Halothane, Acetone, And Ethanol Vapors In The Airways Of The C57bl/6j Mouse, 2014 University of Connecticut
Assessing The Relationship Between The Blood-Air Partition Coefficient And Fractional Uptake Of Inspired Halothane, Acetone, And Ethanol Vapors In The Airways Of The C57bl/6j Mouse, Joshua Baruch Baldino
Honors Scholar Theses
The following series of studies investigates the elimination and uptake trends of halothane, acetone, and ethanol vapors in the airways of C57BL/6J mice. These vapors were chosen because they span a wide range of solubilities, as indicated by their blood-air partition coefficients, and are not associated with any significant airway metabolism or reactivity with tissue substrates in vivo. Mice were exposed to a homogeneous vapor mixture containing a 1:1:1 ratio of halothane, acetone, and ethanol at relative concentrations of approximately 10 ppm. Exposure studies were performed with mice in two states, conscious and deceased, in order to ...
The Fenzian Unit: Applications To Physical Therapy And Beyond, 2014 Liberty University
The Fenzian Unit: Applications To Physical Therapy And Beyond, Jeshua Paul Spadino Mr.
Senior Honors Theses
The field of physical therapy includes many different treatment options in the form of exercises and modalities. The development of a low electrical stimulation signal through the Fenzian unit has provided a new outlook on healing. This device gives information to and receives information from the central nervous system (CNS) through electro-biofeedback and is separated into its own category of modalities. The device can be used to treat a wide range of ailments, all with no adverse effects. This makes it a promising tool in the medical field. It is currently being validated as an authentic medical device through clinical ...
Measured Expiratory Resistance Of The Blue And Green Acapella Devices As Setting Is Increased From 1–5; Amplitude 20, 30, 40, Amanda Wroblewski, Scott Hawkins
College of Health Sciences Presentations
Background: After reviewing the literature on both high flow and low flow Acapella devices, it was determined that most researchers evaluated only three frequency dial settings. Due to this gap, we chose to determine the expiratory resistance at each frequency dial setting for both high flow and low flow Acapella devices, as patient effort (amplitude) is increased on an electronic lung simulator. Hypothesis: 1) As the frequency dial setting is increased on the Acapella, the expiratory resistance will increase, and 2) as the patient effort (amplitude) is increased, the expiratory resistance will increase.
Methods: Each Acapella device was attached, separately ...
Brain Science And Stem Learning, 2014 University of Nebraska - Lincoln
Brain Science And Stem Learning, Dennis Molfese
DBER Speaker Series
Brain Science is an interdisciplinary science and as such touches on a host of domains that among others include Behavioral & Social Sciences, Biological Sciences, Computer & Information Science, Economics, Engineering (multiple domains from Electrical to Mechanical to Structural), Education, Human Resources, Engineering, Environmental, Education, Geosciences, Engineering, Mathematical, Physical Sciences, Psychology, and Zoology. The approach provides a means to study matter from an assortment of elements to the integration of complex domains that stretch from the interactions of neuropeptides to conscious thought that cover the lifespan of single cell organisms to humans. We will touch on a few of these dimensions, addressing ...
Improving Anatomical Knowledge Retention In Medical Students Entering The Surgery And Obstetrics And Gynecology Clinical Rotations By Nesting Interactive Modules, 2014 Himmelfarb Health Sciences Library, The George Washington University
Improving Anatomical Knowledge Retention In Medical Students Entering The Surgery And Obstetrics And Gynecology Clinical Rotations By Nesting Interactive Modules, Samantha Ahle, Jill Krapf, Kirsten Marie Brown, Juliet Lee, Gisela Butera, Ellen Goldman, Myriam Ferzli, Rosalyn A. Jurjus
GW Research Days 2014
Background: It is well documented in the medical education literature that students retain minimal anatomy knowledge when transitioning from pre-clinical didactics to clinical application on the wards. In a previous study at our own institution we were able to quantify this knowledge deficit in students on surgical clerkships, specifically surgery and obstetrics and gynecology (OB/GYN). Using our previous data, we designed an integrated, nested clinical teaching model utilizing e-modules to review clinically relevant surgical anatomy during their clinical rotations.
Objective: The goal of this study is to evaluate the impact of a newly designed method of teaching clinically relevant ...
Mechanisms Associated With The Regulation Of Vascular Structure And Function In Humans, 2014 McMaster University
Mechanisms Associated With The Regulation Of Vascular Structure And Function In Humans, Lisa Cotie
Open Access Dissertations and Theses
A comprehensive understanding of the mechanisms regulating vascular structure and function may assist in designing effective strategies to decrease cardiovascular disease risk. The current studies were designed to investigate a) relationships between collagen markers and arterial stiffness and markers of vasoconstriction and inflammation and endothelial function in humans with a wide range of vascular health, including overweight women, elderly healthy men, individuals with coronary artery disease, individuals with spinal cord injury and young healthy men and b) changes in arterial structure and function and circulating serum markers of type I collagen synthesis and degradation, vasoconstriction and inflammation in overweight pre-menopausal ...