The Effect Of Chronic Sleep Deprivation On Tumor Necrosis Factor Alpha And Bone Health In Peri-Menopausal Rats, Megan Robertson, Derek Booth, Erin Wainwright, Cody Arbuckle, Frank Frisch
Undergraduate Student Research Day Abstracts and Posters
Post-menopausal osteoporosis is a common syndrome in the United States. The cessation of estrogen signaling coupled with the production of pro-inflammatory cytokines from sleep deprivation leads to an even greater risk of post-menopausal osteoporosis by creating an imbalance between osteoblasts and osteoclasts. With estrogen no longer present to regulate the concentration of osteoclasts and pro-inflammatory cytokines increasing production of osteoclasts, bone is degraded at a faster rate than it is formed. One of the most common treatments for osteoporosis is Zolendronate (a nitrogenous bisphosphonate), which decreases the number of osteoclasts in bone. This preliminary study looked at the effects on ...
Structure-Based Network Analysis Of Activation Mechanisms In The Erbb Family Of Receptor Tyrosine Kinases: The Regulatory Spine Residues Are Global Mediators Of Structural Stability And Allosteric Interactions, 2014 Chapman University
Structure-Based Network Analysis Of Activation Mechanisms In The Erbb Family Of Receptor Tyrosine Kinases: The Regulatory Spine Residues Are Global Mediators Of Structural Stability And Allosteric Interactions, Kevin A. James, Gennady M. Verkhivker
School of Computational Science Faculty Articles and Research
The ErbB protein tyrosine kinases are among the most important cell signaling families and mutation-induced modulation of their activity is associated with diverse functions in biological networks and human disease. We have combined molecular dynamics simulations of the ErbB kinases with the protein structure network modeling to characterize the reorganization of the residue interaction networks during conformational equilibrium changes in the normal and oncogenic forms. Structural stability and network analyses have identified local communities integrated around high centrality sites that correspond to the regulatory spine residues. This analysis has provided a quantitative insight to the mechanism of mutation-induced ‘‘superacceptor’’ activity ...
Effect Of Age On Uterine And Ovarian Morphology With Polycystic Ovaries, 2014 The Aga Khan University
Effect Of Age On Uterine And Ovarian Morphology With Polycystic Ovaries, Ambreen Usmani, Rehana Rehman, Aisha Qamar
Department of Biological & Biomedical Sciences
Objective: To measure the outcome of age on ovarian and uterine morphology in primary infertile women with
Methods: The observational cross-sectional study was conducted from January 2009 to March 2010, and the
subjects were recruited from Ziauddin Hospital, Nazimabad, Karachi. The primary infertile subjects with polycystic
ovaries were subdivided into two age groups:20-30 years (group I), and 31-40 years (group II). Both groups had equal
number of subjects. The ovarian volume, follicles count and size, uterine area and endometrial thickness were
determined by transabdominal and transvaginal scans. Shapiro-Wilk's test and Mann-Whitney test were applied,
Effects Of Yoga On Arm Volume Among Women With Breast Cancer Related Lymphedema: A Pilot Study, 2014 University of Dayton
Effects Of Yoga On Arm Volume Among Women With Breast Cancer Related Lymphedema: A Pilot Study, Mary Insana Fisher, Betsy Donahoe-Fillmore, Laura Leach, Colleen O'Malley, Cheryl Paeplow, Tess Prescott, Harold Merriman
Department of Physical Therapy Faculty Publications
Lymphedema affects 3–58% of survivors of breast cancer and can result in upper extremity impairments. Exercise can be beneficial in managing lymphedema. Yoga practice has been minimally studied for its effects on breast cancer related lymphedema (BCRL). The purpose of this study was to determine the effect of yoga on arm volume, quality of life (QOL), self-reported arm function, and hand grip strength in women with BCRL. Six women with BCRL participated in modified Hatha yoga 3×/week for 8 weeks. Compression sleeves were worn during yoga sessions. Arm volume, QOL, self-reported arm function, and hand grip strength were ...
What Are The Best Skeletal Indicators Of Handedness?, 2014 Cleveland State University
What Are The Best Skeletal Indicators Of Handedness?, Margret B. White, Anne Su
Undergraduate Research Posters 2014
Previous research indicates that there are asymmetries in limb bone structure and dimensions. It is hypothesized that these asymmetries are the result of hand preference, or repeated unilateral mechanical loading. The aim of this study was to first identify the best skeletal indicators of handedness by means of a comprehensive literature search. Based on the previous findings of other researchers, we examined non-pathological male individuals (N=19, aged 20-35) from the Hamann-Todd Skeletal Collection at the Cleveland Museum of Natural History for asymmetry of paired second metacarpals, by measuring the difference between right and left diameters at mid-shaft. We also ...
Enamel Matrix Derivative Has No Effect On The Chondrogenic Differentiation Of Mesenchymal Stem Cells, 2014 Royal College of Surgeons in Ireland
Enamel Matrix Derivative Has No Effect On The Chondrogenic Differentiation Of Mesenchymal Stem Cells, Lisanne C. Groeneveldt, Callie Knuth, Janneke Witte-Bourna, Fergal O'Brien, Eppo B. Wolvius, Eric Farrell
Background: Treatment of large bone defects due to trauma, tumor resection, or congenital abnormalities is challenging. Bone tissue engineering using mesenchymal stem cells (MSCs) represents a promising treatment option. However, the quantity and quality of engineered bone tissue are not sufficient to fill large bone defects. The aim of this study was to determine if the addition of enamel matrix derivative (EMD) improves in vitro chondrogenic priming of MSCs to ultimately improve in vivo MSC mediated endochondral bone formation.
Methods: MSCs were chondrogenically differentiated in 2.0 × 105 cell pellets in medium supplemented with TGFβ3 in the absence or presence ...
Surgical Approach In Total Hip Arthroplasty: Patient Outcomes And Impact On Costs, 2014 Western University
Surgical Approach In Total Hip Arthroplasty: Patient Outcomes And Impact On Costs, Stephen M. Petis
University of Western Ontario - Electronic Thesis and Dissertation Repository
Total hip arthroplasty is often renowned as one of the most important surgical advances of the past century. Orthopaedic surgeons must choose a surgical approach to gain access to the hip joint in order to perform the reconstruction. There is debate in the literature as to which surgical approach optimizes patient outcomes, minimizes complications, and reduces costs to hospitals as a high volume procedure.
In the current studies, patient reported outcomes were compared at short-term follow-up using a prospective study design across the anterior, posterior, and lateral approach. A micro-costing method was used to acquire costs related to each procedure ...
Vascular Architecture Of The Liver In Shr And Wky Rats, 2014 Minnesota State University, Mankato
Vascular Architecture Of The Liver In Shr And Wky Rats, Charalette Mathwig, Kenneth Willaert
Journal of Undergraduate Research at Minnesota State University, Mankato
The liver is a highly complex vascular organ containing an intricate network of arteries, arterioles, portal veins, veins, venules, and capillaries. This complex network could change in situations related to vascular disease such as hypertension. We examined the vasculature in rat livers by scanning electron microscopy. The tissue was prepared by perfusing either Mercox resin or polyurethane resin into the vasculature. Once the resin polymerized, each liver was placed in concentrated potassium hydroxide to corrode the tissue from the vascular casts. The casts were critical point dried, sputter coated with gold-palladium, and viewed by scanning electron microscopy. Examination of the ...
Simultaneous Measurement Of Smoothened Entry Into And Exit From The Primary Cilium, 2014 Himmelfarb Health Sciences Library, The George Washington University
Simultaneous Measurement Of Smoothened Entry Into And Exit From The Primary Cilium, Jynho Kim, Elaine Y.C. Hsia, James Kim, Navdar Sever, Philip A. Beachy, Xiaoyan Zheng
Anatomy and Regenerative Biology Faculty Publications
Ciliary accumulation of signaling proteins must result from a rate of ciliary entry that exceeds ciliary exit, but approaches for distinguishing ciliary entry vs. exit are lacking. Using a photoconvertible fluorescent protein tag, we establish an assay that allows a separate but simultaneous examination of ciliary entry and exit of the Hedgehog signaling protein Smoothened in individual cells. We show that KAAD-cyclopamine selectively blocks entry, whereas ciliobrevin interferes initially with exit and eventually with both entry and exit of ciliary Smoothened. Our study provides an approach to understanding regulation of ciliary entry vs. exit of Hedgehog signaling components as well ...
Pathscan Enabler At Md Anderson Cancer Center, 2014 The University of Texas
Pathscan Enabler At Md Anderson Cancer Center, George Mcnamara
McNamara 20140703 - Additional Pathscan and Tiki_Goddess related resources
Our Pathscan Enabler IV, delivered July 2014, uses the QuickScan software to scan a histology slide in "two clicks" (after loading the
1. Push the QuickScan button on the front of the Pathscan Enabler.
2. Click the Scan button in the QuickScan pop-up application (optional: change scan area in the Prescan image window).
The image gets saved as a TIFF file to ...
Cryptorchidism And Infertility In Rats With Targeted Disruption Of The Adamts16 Locus, 2014 Chapman University
Cryptorchidism And Infertility In Rats With Targeted Disruption Of The Adamts16 Locus, Shakila Abdul-Majeed, Blair Mell, Surya M. Nauli, Bina Joe
Pharmacy Faculty Articles and Research
A Disintegrin And Metalloproteinase with ThromboSpondin motifs16 (ADAMTS-16) is a member of a family of metalloproteinases. Using a novel zinc-finger nuclease based gene-edited rat model harboring a targeted mutation of the Adamts16 locus, we previously reported this gene to be linked to blood pressure regulation. Here we document our observation with this model that Adamts16 is essential for normal development of the testis. Absence of Adamts16 in the homozygous Adamts16(mutant) males resulted in cryptorchidism and male sterility. Heterozygous Adamts16(mutant) males were normal, indicating that this is a recessive trait. Testes of homozygous Adamts16(mutant) males were significantly smaller ...
Interaction Among Gustation, Olfaction, And Vision In Flavor Identification, 2014 California Polytechnic State University
Interaction Among Gustation, Olfaction, And Vision In Flavor Identification, Michael J. Lauth
Even though the senses of taste, smell, and sight are distinct, there is a significant overlap among them in our perceptions of objects that helps us understand and differentiate the world. Everyone has experienced, when his or her nose gets congested, that his or her sense of taste changes as well. Many individuals do not equally understand the top-down processing with taste when someone sees objects they are about to eat. In the replicated study by our principle investigator, a random convenience sample of young adults (n=162) were recruited and tested to determine if they could taste four Jelly ...
Dreams And Learning, 2014 California Polytechnic State University
Dreams And Learning, Cody Mebane Gibbons
Project Summary: This study is being done to test one aspect of how paying attention to one’s dreams may influence our waking lives. The idea was inspired by research linking the brain processes involved in long-term memory storage to qualities of dreaming, as well as the potential for learning in lucid dreams. It is hypothesized that the more conscious one is of one’s dreams and dream world, the better one will be at learning. In order to test this, the dreaming ability of 300 Cal Poly students will be analyzed via dream questionnaires with the purpose of seeing ...
Chylomicrons Produced By Caco-2 Cells Contained Apob-48 With Diameter Of 80-200 Nm, 2014 Chapman University
Chylomicrons Produced By Caco-2 Cells Contained Apob-48 With Diameter Of 80-200 Nm, Andromeda M. Nauli, Yuxi Sun, Judy D. Whittimore, Seif Atyia, Guha Krishnaswamy, Surya M. Nauli
Pharmacy Faculty Articles and Research
The small intestine generally transports dietary fats to circulation in triglyceride (TG)-rich lipoproteins. The two main intestinal lipoproteins are chylomicron (CM) and very low-density lipoprotein (VLDL). Unfortunately, studies on the CM biogenesis and intestinal transport of dietary fats have been hampered by the lack of an adequate in vitro model. In this study, we investigated the possible factors that might increase the efficiency of CM production by Caco-2 cells. We utilized sequential NaCl gradient ultracentrifugation to isolate the CMs that were secreted by the Caco-2 cells. To confirm the successful isolation of the CMs, we performed Fat Red 7B ...
Endothelial And Smooth Muscle-Dependent Vascular Reactivity In Immature Arterialized Collateral Capillaries, 2014 California Polytechnic State University
Endothelial And Smooth Muscle-Dependent Vascular Reactivity In Immature Arterialized Collateral Capillaries, Caitlin Koeroghlian
Biomedical Eng/General Eng.
Peripheral arterial occlusive disease (PAOD) occurs due to the build up of atherosclerotic plaque and reduces blood flow to cause chronic ischemia. Patients with PAOD may experience intermittent claudication, or the pain in limb skeletal muscles due to a decease in blood flow. Collateral arteries can act as a natural bypass and improve blood flow to hypoxic tissue by creating an alternate route for blood to flow, but not all patients with PAOD have pre-existing collateral networks. Animal studies indicate that tissues without pre-existing collateral networks can form de novo collaterals from capillaries following occlusion of a feed artery. Unfortunately ...
Vancomycin-Bearing Synthetic Bone Graft Delivers Rhbmp-2 And Promotes Healing Of Critical Rat Femoral Segmental Defects, 2014 University of Massachusetts Medical School
Vancomycin-Bearing Synthetic Bone Graft Delivers Rhbmp-2 And Promotes Healing Of Critical Rat Femoral Segmental Defects, Jordan D. Skelly, Jeffrey Lange, Tera M. Filion Potts, Xinning Li, David C. Ayers, Jie Song
UMass Center for Clinical and Translational Science Research Retreat
For graft-assisted repair of large volumetric bone loss resulting from traumatic orthopedic injuries, strategies that simultaneously promote osteointegration/graft healing and mitigate risks for infections are highly desired. Previously, we developed a poly(2-hydroxyethyl methacrylate)-nanocrystalline hydroxyapatite (pHEMA-nHA) composite as a synthetic bone graft. The composite, when loaded with a single dose of 400-ng rhBMP-2/7 and press-fit into a 5-mm rat femoral segmental defect, led to bony callus fully bridging over the defect and substantial restoration of the torsional rigidity by 12 weeks. More recently, we showed that 4.8 wt% vancomycin can be encapsulated within the composite without ...
Differential Burden Of Musculoskeletal Pain In Blacks And Whites At The Time Of Total Joint Replacement Surgery, 2014 University of Massachusetts Medical School
Differential Burden Of Musculoskeletal Pain In Blacks And Whites At The Time Of Total Joint Replacement Surgery, Anthony Porter, Leslie R. Harrold, Philip C. Noble, David C. Ayers, Patricia D. Franklin, Jeroan Allison
UMass Center for Clinical and Translational Science Research Retreat
Introduction: The existence of racial disparities in total knee (TKR) and hip (THR) replacement outcomes is well established. The role of musculoskeletal co-morbidities among black and white TKR patients at the time of surgery were investigated in a prospective cohort enrolled in the FORCE-TJR consortium of 131 surgeons in 22 US states. Materials & methods: Descriptive analyses were performed on 3,306 TKR and 2,439 THR patients. Data included sociodemographic factors (age, sex, race), BMI, comorbid conditions using the modified Charlson comorbidity scores, burden of musculoskeletal disease using the Knee/Hip injury and Osteoarthritis Outcome Score (KOOS/HOOS) in both knees and hips, emotional health based on the Short Form 36 (SF-36) Mental Component Score (MCS) and physical function based on the Physical Component Score (SF-36 PCS). Factors associated with pre-operative surgical joint pain and function were examined using multivariate stepwise linear regression models. Results: Compared to Whites, Blacks (143 hips and 201 knees) reported worse surgical joint pain (mean pain: 39.3 vs. 49.2 (hip); 43.4 vs. 53.2 (knee)), poorer surgical joint function (mean function: 38.9 vs. 45.7 (hip); 45.9 vs. 53.4 (knee)), poorer global function (mean PCS: 30.0 vs. 31.6 (hip); 31.3 vs. 33.1 (knee)), and more non-operative joints pain. (All ...
Displacement Across A Fracture Gap With Axial Loading Of Far Cortical Locking Constructs, 2014 University of Massachusetts Medical School
Displacement Across A Fracture Gap With Axial Loading Of Far Cortical Locking Constructs, Peter J. Wagner, Dennis T. Murphy, Kristen L. Billiar, Judith Siegel, John J. Wixted
UMass Center for Clinical and Translational Science Research Retreat
Purpose: Far cortical locking has been proposed for reducing stiffness and promoting greater dynamic stability in locked plating constructs. Prior studies have shown reduced stiffness with axial loading of these constructs, leading to a theoretical increase in inter-fragmentary motion and secondary bone healing. The purpose of this study was to examine strain across a fracture gap using far cortical locking constructs in a biomechanical model of distal femoral fractures.
Methods: Fourth generation sawbones were cut transversely along the distal diaphysis and plated with distal femoral buttress plates and cortical locking screws. Far cortical locking (FCL) specimens were predrilled in the ...
Activation Of C-Met And Upregulation Of Cd44 Expression Are Associated With The Metastatic Phenotype In The Colorectal Cancer Liver Metastasis Model, Victoria Allison Elliott, Piotr G. Rychahou, Yekaterina Y. Zaytseva, B. Mark Evers
Markey Cancer Center Faculty Publications
Liver metastasis is the most common cause of death in patients with colorectal cancer. Despite extensive research into the biology of cancer progression, the molecular mechanisms that drive colorectal cancer metastasis are not well characterized.
HT29 LM1, HT29 LM2, HT29 LM3 cell lines were derived from the human colorectal cancer cell line HT29 following multiple rounds of in vivo selection in immunodeficient mice.
CD44 expression, a transmembrane glycoprotein involved in cell-cell and cell-matrix adhesions, and cancer cells adhesion to endothelial cells was increased in all in vivo selected cell lines, with maximum CD44 expression and cancer cells ...
A Comparison Of Angiography Versus Intravascular Ultrasound In The Treatment Of Peripheral Arterial Disease, 2014 University of South Carolina
A Comparison Of Angiography Versus Intravascular Ultrasound In The Treatment Of Peripheral Arterial Disease, Michael Pompliano
Peripheral Arterial Disease is a growing epidemic throughout the United States. It is estimated that 8 to 12 million Americans currently suffer from PAD, a disease of the circulatory system that limits blood flow to your hands and feet. This limited blood flow is due to the narrowing of the arteries that supply blood throughout your body and can disrupt the balance of the nerves and tissues that make up your extremities. If left untreated, it can cause irreparable, life- threatening damage that may result in amputation of the diseased limb. Although the mechanism of PAD is known and well ...