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Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons™
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Full-Text Articles in Analytical, Diagnostic and Therapeutic Techniques and Equipment
Assessing Daylight & Low-Dose Rate Photodynamic Therapy Efficacy, Using Biomarkers Of Photophysical, Biochemical And Biological Damage Metrics In Situ., Ana Luiza Ribeiro De Souza, Ethan Larochelle, Kayla Marra, Jason Gunn, Scott C. Davis, Kimberley S. Samoke, M. Shane Chapman, Edward V. Maytin, Tayyaba Hasan, Brian W. Pogue
Assessing Daylight & Low-Dose Rate Photodynamic Therapy Efficacy, Using Biomarkers Of Photophysical, Biochemical And Biological Damage Metrics In Situ., Ana Luiza Ribeiro De Souza, Ethan Larochelle, Kayla Marra, Jason Gunn, Scott C. Davis, Kimberley S. Samoke, M. Shane Chapman, Edward V. Maytin, Tayyaba Hasan, Brian W. Pogue
Dartmouth Scholarship
Background
Sunlight can activate photodynamic therapy (PDT), and this is a proven strategy to reduce pain caused by conventional PDT treatment, but assessment of this and other alternative low dose rate light sources, and their efficacy, has not been studied in an objective, controlled pre-clinical setting. This study used three objective assays to assess the efficacy of different PDT treatment regimens, using PpIX fluorescence as a photophysical measure, STAT3 cross-linking as a photochemical measure, and keratinocyte damage as a photobiological measure.
Methods
Nude mouse skin was used along with in vivo measures of photosensitizer fluorescence, keratinocyte nucleus damage from pathology, …