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Full-Text Articles in Analytical, Diagnostic and Therapeutic Techniques and Equipment
Immunologic Risk Prediction Model For Kidney Graft Function, Christina Diane Bishop
Immunologic Risk Prediction Model For Kidney Graft Function, Christina Diane Bishop
Doctoral Dissertations
Clinicians lack appropriate non-invasive methods to be able to predict, diagnose, and reduce the risk of rejection in the years following kidney transplantation. Protocol biopsies and monitoring of serum creatinine levels are the most common methods of monitoring graft function after transplant; however, they have several negative aspects. Use of traditional factors regarding donors and recipients such as Human Leukocyte Antigen (HLA) DNA typing, pre-transplant anti-HLA antibody levels, and basic demographics (age, ethnicity/race, gender), has proved inadequate for post-transplant graft monitoring past the first few years. We propose that by utilizing immunologic factors available to clinicians across the United States, …
Why Is There Still So Much Confusion About Vo2 Plateau? A Re-Examination Of The Work Of A.V. Hill, Richard Vincent Castle
Why Is There Still So Much Confusion About Vo2 Plateau? A Re-Examination Of The Work Of A.V. Hill, Richard Vincent Castle
Masters Theses
Maximal oxygen uptake (VO2max) is regarded as the gold standard for assessing aerobic fitness. In 1923, Hill et al. proposed that VO2max represents the maximal ability of the body to take in and consume O2 during strenuous exercise. Recently, however, controversy has arisen over the issue of whether a leveling off, or "plateau" in VO2 is necessary to verify attainment of VO2max. Purpose: To compare two different VO2max protocols and determine if both protocols show direct evidence of an upper limit on VO2. Methods: Nine runners (18-35 years old) completed …
Development Of An Autonomous Mammalian Lux Reporter System, Daniel Michael Close
Development Of An Autonomous Mammalian Lux Reporter System, Daniel Michael Close
Doctoral Dissertations
Since its characterization, the definitive shortcoming of the bacterial luciferase (lux) bioluminescent reporter system has been its inability to express at a functional level in the eukaryotic cellular background. While recent developments have allowed for lux function in the lower eukaryote Saccharomyces cerevisiae, they have not provided for autonomous function in higher eukaryotes capable of serving as human biomedical proxies. Here it is reported for the first time that, through a process of poly-bicistronic expression of human codon-optimized lux genes, it is possible to autonomously produce a bioluminescent signal directly from mammalian cells. The low background of …