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Effect Of Culture Medium Volume On The Development And Viability Of Microinjected Or Noninjected One-Cell Hybrid And Cd-1 Strain Mouse Embryos, Haydar Bağiş, Hande Odaman Mercan

Turkish Journal of Veterinary & Animal Sciences

We investigated the effects of drop volume on the development of mouse presumptive zygotes to the completely hatched blastocyst stage. For this aim, we established 3 experimental groups. In the first group, we cultured hybrid presumptive zygotes to the hatched blastocyst stage in 10 presumptive zygotes per 5, 10, 20, 40 and 50 µl drops. In the second group, we used microinjected hybrid zygotes for culture. In the last group, we cultured CD-1 mouse presumptive zygotes to the hatched blastocyst stage. There was no statistical difference in the first group. In contrast, the data demonstrated that there were significant differences …