Medicine and Health Sciences Commons^™

Allergic Bronchopulmonary Aspergillosis: Radiological And Microbiological Profile Of Patients Presented In An Outpatient Pulmonary Clinic In A Developing Country, Nousheen Iqbal, Muhammad Irfan, Mustafa Bin Ali Zubairi, Maaha Ayub, Safia Awan, Kauser Jabeen, Ali Bin Sarwar Zubairi

Section of Pulmonary & Critical Care

There is limited data available about allergic bronchopulmonary aspergillosis (ABPA) in Pakistan. The aim of the study was to describe the radiological and microbiological profile of ABPA patients presenting to the outpatient pulmonary clinic of a tertiary care hospital in Karachi, Pakistan. A retrospective study was conducted on ABPA patients who presented to the pulmonary outpatient clinic at Aga Khan University Hospital, Karachi, Pakistan, from January 2017 to December 2019. Data was collected on microbiology and radiology features on predesigned proforma. A total of 7759 asthmatic patients presented at the outpatient pulmonology clinic during the study period. Of the 245 …

The Implications Of Ferroptosis In Antibiotic Resistance, Marysol Hohl

Senior Honors Theses

Bacterial infections in the United States are becoming increasingly resistant to existing antibiotic treatments. Due to projected increases in resistance and the recent decrease in novel antibacterials, experts have determined that the United States is in the “post-antibiotic era.” The scientific community has failed to resolve resistance despite the continual discovery of new antibiotic compounds. In the past decade, a novel form of cell death called ferroptosis has been implicated in antibiotic treatment by employing the use of nanotechnology. This literature review will describe the problem of bacterial resistance and demonstrate how current research is pioneering a new age of …

Evaluation Of Glycerol Antiseptic Effect On Gram Positive And Gram Negative Bacteria, Asmaa S. Sleem, Samar S. Salman, Wafaa A. Shehata, Amal M. Dawoud

Menoufia Medical Journal

Objectives: To assess glycerol antibacterial activity against both Gram positive and negative microorganisms, detect impact of increasing glycerol concentration in bacterial cultures on extra/ intracellular Glycerol-3-phosphate (G3P) concentration, evaluate antiseptic efficacy of different glycerol/alcohol formulations and monitor HCWs tolerability to these formulations to recommend the best antiseptic preparation in vitro. Background: The progressively rising nosocomial infections have enforced the need for novel infection prevention modalities. Glycerol emerges as a significant and cheap antibacterial agent against both Gram positive and negative organisms without any toxic effects. Methodology: Different glycerol/ethanol formulations were evaluated for both bacterial inhibitory and bactericidal activity against selected …

Understanding The Consumption Of Antimicrobial Resistance–Related Content On Social Media: Twitter Analysis, Hyunuk Kim, Chris R. Proctor, Dylan Walker, Ronan R. Mccarthy

Business Faculty Articles and Research

Background: Antimicrobial resistance (AMR) is one of the most pressing concerns in our society. Today, social media can function as an important channel to disseminate information about AMR. The way in which this information is engaged with depends on a number of factors, including the target audience and the content of the social media post.

Objective: The aim of this study is to better understand how AMR-related content is consumed on the social media platform Twitter and to understand some of the drivers of engagement. This is essential to designing effective public health strategies, raising awareness about antimicrobial …

Correlation Of Acetylcholinesterase Activity And Bone Biochemical Markers In Premature And Full-Term Neonates, Khaled Mohammed Elsayed, Abd Elaziz Abd Elrahman Alnokaly, Mohammed Abd El-Malik Hassan, Mabrouk Mahmoud Abo Elenin

Al-Azhar International Medical Journal

Background: Prematurity lowers body weight and bone mineral density in nearly 30% of preterm newborns. Premature newborns are not screened for metabolic bone disease, hence bone biochemical markers should be investigated. Acetylcholinesterase mediates bone remodeling in recent investigations. Bone biochemical indicators and acetylcholinesterase (AChE) levels in preterm newborns may be linked. Objective: The aim of our study is to evaluate the probable correlation of AChE levels with multiple bone biomarkers in full-term and premature neonates. Subjects and Methods: This study was designed as a case control study. We examined 80 newborns (40 preterm with gestational age less than 37 weeks, …

Uterine Artery Doppler Indices As Determinant Of Success In Intra Cytoplasmic Sperm Injection, Abd-Elqawy Hendawy Shams, Fahd Abd Elall El Omda, Noha Mohamed Sabry

Al-Azhar International Medical Journal

Background: In-Vitro Fertilization (IVF). Patients with infertility are usually advised to have intracytoplasmic sperm injections (ICSI). Doppler ultrasound, which uses high frequency transvaginal probes and coloured pictures, is one of the most frequently used methods in this field. One of the parameters supplied by an ultrasonographic test for determining the success of IVF includes endometrial thickness, endometrial pattern, endometrial volume, and endometrial blood flow. Aim: To detect implantation window using uterine artery indices in ICSI cycles. Subject and Methods: This study will be concluded at the assisted reproductive techniques unit of AlAzhar University on 100 patients. Results: Regarding BMI, there …

Antimicrobial Resistance In Eskape Pathogens And Its Effect On Modern Medicine And Treatment, Cameran Runge

Honors Theses

Abstract

ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella

pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter

spp.) are seeing a growing resistance to multiple classes of antibiotics. Misuse and overuse of antibiotics have played directly into the resistance observed, and the problem is growing exponentially. Antibiotic resistance is partially due to several intrinsic factors limiting the drug's uptake. These include efflux pumps, increased biofilm production, and reduced cell wall permeability in the resistant bacteria. ESKAPE pathogens also acquire resistance through horizontal gene transfer and plasmids. As antibiotics have become less effective, the bacteria can continue to thrive, leading to a detrimental …

Human Cytomegalovirus (Cmv) Infection Associated With Decreased Risk Of Bronchogenic Carcinoma: Understanding How A Previous Cmv Infection Leads To An Enhanced Immune Response Against Malignancy., Selena Rashid, Amalia Ardeljan, Lexi R. Frankel, Matthew Cardeiro, Enoch Kim, Brittany M. Nagel, Kazuaki Takabe, Omar Rashid

HPD Articles

INTRODUCTION:  Cytomegalovirus (CMV) causes a long-lasting, asymptomatic infection that reportedly has both advantageous and deleterious effects on tumor progression. The purpose of this study was to evaluate the correlation between CMV infection and the incidence of bronchogenic carcinoma.

METHODS: The study was conducted using a Health Insurance Portability and Accountability Act (HIPAA) compliant national database to identify patients both with and without histories of CMV infection using International Classification of Diseases (ICD-10 and ICD-9) codes. Access to the database was granted by Holy Cross Health, Fort Lauderdale for the purpose of academic research with standard statistical methods used to analyze …

The Power And Challenge Of Lipid (A)Symmetry Across The Membrane And Cell, Mikhail Bogdanov

Journal Articles

Membrane asymmetry means that the two sides of membrane are structurally, physically and functionally different. Membrane asymmetry is largely related to the lipid sidedness and particularly to compositional (lipid head and acyl group) and physical (lipid packing order, charge, hydration and H-bonding interactions) differences in the inner and outer leaflets of lipid bilayer. Chemically, structurally and conformationally different non-covalent bound lipid molecules are physically fluid and deformable and enable to interact dynamically to form transient arrangements with asymmetry both perpendicular and parallel to the plane of the lipid bilayer. Although biological membranes are almost universally asymmetric however the asymmetry is …

Protocol To Identify The Core Gene Supported By An Essential Gene In E. Coli Bacteria Using A Genome-Wide Suppressor Screen, Isao Masuda, Ya-Ming Hou

Department of Biochemistry and Molecular Biology Faculty Papers

We describe here a genome-wide screening approach to identify the most critical core reaction among a network of many that are supported by an essential gene to establish cell viability. We describe steps for maintenance plasmid construction, knockout cell construction, and phenotype validation. We then detail isolation of suppressors, whole-genome sequencing analysis, and reconstruction of CRISPR mutants. We focus on E. coli trmD, which encodes an essential methyl transferase that synthesizes m1G37 on the 3'-side of the tRNA anticodon. For complete details on the use and execution of this protocol, please refer to Masuda et al. (2022).

Examining The Effect Of Varying Dilutions Of Octopamine On The Gut Microbiome Of Lab-Raised And Wild Acheta Domesticus By Brandon Nelson University Of Puget Sound Biology, Brandon Nelson

Summer Research

For my research I am comparing the gut microbiome of lab raised A. Domesticus that are given various levels of Octopamine, a stress hormone, and comparing that to the gut microbiome of wild cricket types. Crickets are a widely used organism across the world for various experiments as both subject and feed. Knowing how the gut microbiome of these crickets is affected by various levels of stress will allow us to better take care of these crickets. This could also help us determine if there are any diseases that lab raised crickets may be susceptible to. A large part of …