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Full-Text Articles in Medicine and Health Sciences
Activation Of Extracellular-Signal Regulated Kinase (Erk1/2) By Fluid Shear Is Ca2+- And Atp-Dependent In Mc3t3-E1 Osteoblasts, Dawei Liu, Damian C. Genetos, Ying Shao, Derik J. Geist, Jiliang Li, Hua Zhe Ke, Charles H. Turner, Randall L. Duncan
Activation Of Extracellular-Signal Regulated Kinase (Erk1/2) By Fluid Shear Is Ca2+- And Atp-Dependent In Mc3t3-E1 Osteoblasts, Dawei Liu, Damian C. Genetos, Ying Shao, Derik J. Geist, Jiliang Li, Hua Zhe Ke, Charles H. Turner, Randall L. Duncan
School of Dentistry Faculty Research and Publications
To determine the role of Ca2+ signaling in activation of the Mitogen-Activated Protein Kinase (MAPK) pathway, we subjected MC3T3-E1 pre-osteoblastic cells to inhibitors of Ca2+ signaling during application of fluid shear stress (FSS). FSS only activated ERK1/2, rapidly inducing phosphorylation within 5 min of the onset of shear. Phosphorylation of ERK1/2 (pERK1/2) was significantly reduced when Cai2+ was chelated with BAPTA or when Ca2+ was removed from the flow media. Inhibition of both the L-type voltage-sensitive Ca2+ channel and the mechanosensitive cation-selective channel blocked FSS-induced pERK1/2. Inhibition of phospholipase C with U73122 significantly reduced …
Oxidative Stress And Cell Death In Osmotically Swollen Glial Cells, Crystal Elaine Stuckey
Oxidative Stress And Cell Death In Osmotically Swollen Glial Cells, Crystal Elaine Stuckey
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Regulation of normal volume is an important aspect of cell homeostasis. Possible mechanisms which signal volume regulation are increasing the rate of reactive oxygen species (ROS) production and release of ATP for interaction with purinergic receptors. We examined whether an increase in ROS production during cell swelling also led to cell injury of C6 glioma cells. Cells were loaded with 5,6-carboxy-2,7-dihydrofluorscein diacetate (DCFDA) to fluoroscopically measure the rate of cellular ROS production and were perfused with phosphate-buffered saline solutions (PBS) containing 100 μM carbenoxolone to inhibit dye efflux. Cell death was determined cytometrically and by measuring the release of lactate …