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Full-Text Articles in Medicine and Health Sciences
Expression And Characterization Of Two Alternatively Spliced Isoforms Of Rat Type I Receptor For Inositol 1,4,5-Trisphosphate, Doris Da May Lin
Expression And Characterization Of Two Alternatively Spliced Isoforms Of Rat Type I Receptor For Inositol 1,4,5-Trisphosphate, Doris Da May Lin
Yale Medicine Thesis Digital Library
The transmembrane receptor for IP3 is a tetrameric calcium channel located in the endoplasmic reticulum. In many cell types the receptor mediates such complex signals as calcium waves and oscillations. There are multiple receptor isoforms, derived by alternative splicing as well as different genes. The functional significance of these many receptor isoforms has not been determined. To this end, I have generated cell lines that express homotetrameric receptors.Stable lines of the human embryonic kidney (HEK293) cells were established by transfection with cDNAs encoding for two splice variants of the rat type I receptor. The two variants differ in the presence …
The Spleen Focus-Forming Virus Envelope Glycoprotein, Gp55, And The Block In The Erythropoietin-Induced Differentiation Of Friend Murine Erythroleukemia Cells, Thomas Samuel Lin
The Spleen Focus-Forming Virus Envelope Glycoprotein, Gp55, And The Block In The Erythropoietin-Induced Differentiation Of Friend Murine Erythroleukemia Cells, Thomas Samuel Lin
Yale Medicine Thesis Digital Library
Unlike normal committed erythroid progenitor cells, which require erythropoietin (EPO) for both proliferation and terminal differentiation, Friend murine erythroleukemia (MEL) cells are EPO-independent and do not differentiate in response to the cytokine. Dimethylsulfoxide (DMSO) induces differentiation and restores responsiveness to EPO. DMSO-treated Friend cells responded to EPO with both increased proliferation and further hemoglobin synthesis. The effects of DMSO upon the expression of EPO receptor (EPOR) mRNA and cell surface receptor were examined. Clone DMSO/R1, an MEL subline resistant to the differentiation-inducing and EPO-sensitizing effects of DMSO, expressed a higher level of EPOR mRNA than parental Friend cells, and DMSO …
The Effect Of Selenium Supplementation On The Immune Response Of Mice With Experimental Chagas' Disease, Cindy Calisi, Lynette Brooks
The Effect Of Selenium Supplementation On The Immune Response Of Mice With Experimental Chagas' Disease, Cindy Calisi, Lynette Brooks
Mahurin Honors College Capstone Experience/Thesis Projects
No abstract provided.
The Identification And Characterization Of Protein Tyrosine Phosphatases Expressed In The Developing Rat Brain, Mustafa Sahin
The Identification And Characterization Of Protein Tyrosine Phosphatases Expressed In The Developing Rat Brain, Mustafa Sahin
Yale Medicine Thesis Digital Library
Previous studies of the developing nervous system have shown that cell-cell and cell-matrix interactions are involved in a variety of processes such as the proliferation, migration and differentiation of neurons. While many cell surface molecules have been identified, the signal transduction mechanisms through which they alter cellular responses are poorly understood. Recent studies have described a new and large family of enzymes, protein tyrosine phosphatases (PTPases), that may play a key role in transduction of cell surface events. Opposing the actions of protein tyrosine kinases (PTKs), PTPases may determine the state of tyrosine phosphorylation of a protein and regulate its …