Medicine and Health Sciences Commons^™

Alcohol And Cannabinoids Differentially Affect Hiv Infection And Function Of Human Monocyte-Derived Dendritic Cells(Mddc), Marisela Agudelo, Gloria Figueroa, Adriana Yndart-Arias, Gianna Casteleiro, Karla Muñoz, Thangavel Samikkannu, Venkata Atluri, Madhavan P. Nair

HWCOM Faculty Publications

During human immunodeficiency virus (HIV) infection, alcohol has been known to induce inflammation while cannabinoids have been shown to have an anti-inflammatory role. For instance cannabinoids have been shown to reduce susceptibility to HIV-1 infection and attenuate HIV replication in macrophages. Recently, we demonstrated that alcohol induces cannabinoid receptors and regulates cytokine production by monocyte-derived dendritic cells (MDDC). However, the ability of alcohol and cannabinoids to alter MDDC function during HIV infection has not been clearly elucidated yet. In order to study the potential impact of alcohol and cannabinoids on differentiated MDDC infected with HIV, monocytes were cultured for 7 …

Black–White Latino Racial Disparities In Hiv Survival, Florida, 2000–2011, Diana M. Sheehan, Mary Jo Trepka, Kristopher P. Feenie, Guillermo Prado, Miguel Angel Cano, Lorene M. Maddox

Department of Epidemiology

This research aimed to estimate Black/White racial disparities in all-cause mortality risk among HIV-positive Latinos. Florida surveillance data for Latinos diagnosed with HIV (2000–2008) were merged with 2007–2011 American Community Survey data. Crude and adjusted hazard ratios (aHR) were calculated using multi-level Cox regression. Of 10,903 HIV-positive Latinos, 8.2% were Black and 91.9% White. Black Latinos were at increased mortality risk compared with White Latinos after controlling for individual and neighborhood factors (aHR 1.40, 95% confidence interval (CI) 1.21–1.62). In stratified analyses, risk factors for Black Latinos included: age ¥60 years compared with ages 13–19 (aHR 4.63, 95% CI 1.32–16.13); …

“That’S True Love:” Lived Experiences Of Puerto Rican Perinatally Hiv-Infected Youth Within Their Families’ Context, Georgina Silva-Suarez, Elena Bastida, Silvia E. Rabionet, Consuelo Beck Sague, Irma Febo, Carmen D. Zorrilla

Department of Health Promotion and Disease Prevention

The burden of HIV affects not only HIV-infected patients but also their families and caregivers. It is also known that family support is crucial for people living with HIV. A qualitative study was conducted to explore the life experiences, within the family context, of perinatally HIV-infected (pHIV-I) youth in Puerto Rico. Twenty in-depth interviews were performed and audio-recorded. Within the family context, study participants experienced acceptance, love and support but also stigma and discrimination. They reported that family is an essential component in their lives and treatment. Losing one or both parents at a young age was considered more difficult …

Impact Of Sperm Dna Chromatin In The Clinic, Dimitirios Ioannou, David Miller, Darren K. Griffin, Helen G. Tempest

Biomolecular Sciences Institute: Faculty Publications

The paternal contribution to fertilization and embryogenesis is frequently overlooked as the spermatozoon is often considered to be a silent vessel whose only function is to safely deliver the paternal genome to the maternal oocyte. In this article, we hope to demonstrate that this perception is far from the truth. Typically, infertile men have been unable to conceive naturally (or through regular IVF), and therefore, a perturbation of the genetic integrity of sperm heads in infertile males has been under-considered. The advent of intracytoplasmic sperm injection (ICSI) however has led to very successful treatment of male factor infertility and subsequent …

Logic Regression-Derived Algorithms For Syndromic Management Of Vaginal Infections, Sujit D. Rathod, Tan Li, Jeffery D. Klausner, Alan Hubbard, Arthur L. Reingold, Purnima Madhivanan

Department of Biostatistics Faculty Publications

Background: Syndromic management of vaginal infections is known to have poor diagnostic accuracy. Logic regression is a machine-learning procedure which allows for the identification of combinations of variables to predict an outcome, such as the presence of a vaginal infection.

Methods: We used logic regression to develop predictive models for syndromic management of vaginal infection among symptomatic, reproductive-age women in south India. We assessed the positive predictive values, negative predictive values, sensitivities and specificities of the logic regression procedure and a standard WHO algorithm against laboratory-confirmed diagnoses of two conditions: metronidazole-sensitive vaginitis [bacterial vaginosis or trichomoniasis (BV/TV)], and vulvovaginal candidiasis …

A Novel Biomarker Of Oxidative Stress Is Associated With Risk Of Death In Patients With Coronary Artery Disease, Riyaz S. Patel, Nima Ghasemzadeh, Danny J. Eapen, Salman Sher, Shawn Arshad, Yi-An Ko, Emir Veledar, Habib Samady, A. Maziar Zafari, Laurence Sperling, Viola Vaccarino

Department of Biostatistics Faculty Publications

Background—Free radical scavengers have failed to improve patient outcomes promoting the concept that clinically important oxidative stress (OS) may be mediated by alternative mechanisms. We sought to examine the association of emerging aminothiol markers of non-free radical mediated oxidative stress with clinical outcomes.

Methods and Results—Plasma levels of reduced (cysteine and glutathione) and oxidized (cystine and glutathione disulphide) aminothiols were quantified by high performance liquid chromatography in 1411 patients undergoing coronary angiography (mean age 63 years, male 66%). All patients were followed for a mean of 4.7±2.1 years for the primary outcome of all-cause death (n=247). Levels of …

Impact Of Donor Lung Gram Stain On Post-Transplant Pneumonia And Mortality: More Lungs Can Be Used To Save Lives, Sher Ali Khan, Emir Veledar, Cathy Logan, Sanjay Mehta, Gordon Yung, Saima Aslam

Department of Biostatistics Faculty Publications

No abstract provided.

A Team Reacts To A Patient’S Death, Suzanne Minor, Sanaz Kashan, Maite Castillo

HWCOM Faculty Publications

No abstract provided.

Us Veterans And Their Unique Issues: Enhancing Health Care Professional Awareness, Maria Olenick, Monica Flowers, Valerie J. Diaz

Nicole Wertheim College of Nursing and Health Sciences

United States veterans are a multifaceted population with a distinct culture that includes, but is not limited to, values, customs, ethos, selfless duty, codes of conduct, implicit patterns of communication, and obedience to command. Veterans experience mental health disorders, substance use disorders, post-traumatic stress, and traumatic brain injury at disproportionate rates compared to their civilian counterparts. Eighteen to 22 American veterans commit suicide daily and young veterans aged 18–44 are most at risk. Health care professionals must be aware of patients’ military history and be able to recognize suicide-risk factors, regardless of age. Advancement in medical technology has allowed servicemen …

Infant And Child Deaths: Parent Concerns About Subsequent Pregnancies, Dorothy Brooten, Joanne M. Youngblut, Jean Hannan, Carmen Caicedo, Rosa M. Roche, Fatima Malkawi

Nicole Wertheim College of Nursing and Health Sciences

Purpose—examine parents' concerns about subsequent pregnancies after experiencing an infant or child death (newborn to 18 years). Data Sources—39 semi-structured parent (White, Black, Hispanic) interviews 7 and 13 months post infant/child death conducted in English and/or Spanish, audio-recorded, transcribed and content analyzed. Mothers' mean age was 31.8 years, fathers' was 39 years; 11 parents were White, 16 Black, 12 Hispanic. Conclusions—Themes common at 7 and 13 months: wanting more children; fear, anxiety, scared; praying to God/God's will; thinking about/keeping the infant's/child's memory and at 7 months importance of becoming pregnant for family members; and at 13 months happy about a …

Transitioning From Military Medics To Registered Nurses, Mohamed D. Keita, Valerie J. Diaz, Audrey P. Miller, Maria Olenick, Sharon R. Simon

Nicole Wertheim College of Nursing and Health Sciences

The nursing shortage in the USA is expected to reach 260,000 registered nurses (RNs) by 2025. The most profound shortages are expected in California and Florida, translating into 109,779 and 128,364 RN jobs, respectively. Despite a foreseen growth in nursing career opportunities nationwide, the supply of nurses will be insufficient to meet the corresponding demand. Capitalizing on prior education, experience, and skills of military clinical personnel to fill these jobs could significantly reduce the projected nursing shortage. Florida International University’s Nicole Wertheim College of Nursing and Health Sciences is circumventing barriers to recruit, retain, and graduate transitioning veteran medics and …

Triple Negative Breast Cancers Express Receptors For Lhrh And Are Potential Therapeutic Targets For Cytotoxic Lhrh-Analogs, Aezs 108 And Aezs 125, Stephen Seitz, Stefan Buchholz, Andrew Victor Schally, Florian Weber, Monika Klinkhammer-Schalke, Elizabeth C. Inwald, Roberto Perez, Ferenc G. Rick, Luca Szakontay, Florian Hohla, Sabine Segerer, Chui Wai Kwok, Olaf Ortmann, Jorg Bernhard Engel

HWCOM Faculty Publications

Background

Triple negative breast cancer (TNBC) is a distinct subtype of breast cancer burdened with a dismal prognosis due to the lack of effective therapeutic agents. Receptors for LHRH (luteinizing hormone-releasing hormone) can be successfully targeted with AEZS-108 [AN-152], an analog of LHRH conjugated to doxorubicin. Our study evaluates the presence of this target LHRH receptor in human specimens of TNBC and investigates the efficacy and toxicity of AEZS-108 in vivo. We also studied in vitro activity of AEZS-125, a new LHRH analog conjugated with the highly potent natural compound, Disorazol Z.

Methods

69 human surgical specimens of TNBC were …

Comparison Of Individual And Pooled Urine Samples For Estimating The Presence And Intensity Of Schistosoma Haematobium Infections At The Population Level, Abraham Degarege, Berhanu Erko, Zeleke Mekonnen, Mengistu Legesse, Yohannes Negash, Jozef Vercruysse, Bruno Levecke

Department of Epidemiology

Background

There is a lack of cost-effective diagnostic strategies to evaluate whether mass drug administration (MDA) programmes to control Schistosoma haematobium progress as anticipated. The purpose of this study is to provide a proof-of-principle for examination of pooled urine samples as a strategy for rapid assessment of presence and intensity of Schistosoma haematobium infections at the population level.

Methods

A total of 640 urine samples were collected from 520 school-aged children (520 at baseline and 120 at follow-up) during a clinical trial that was designed to assess the efficacy of praziquantel against Schistosoma haematobium infections in Ethiopia. Individual and pooled …

Speech-Language Pathology Interns’ Perceptions Of What Supervisors Value Most During Clinical Practicum, Karin Cardozo

FIU Electronic Theses and Dissertations

The purpose of this investigation was to analyze interns’ perceptions of what supervisors considered important supervisory behaviors and to compare those perceptions with what the supervisors considered important. Participants consisted of 33 interns and 23 supervisors. Results of two surveys collected in previous studies were compared and analyzed. Tihen’s (1983) “Tihen’s Student Expectations of their Clinical Supervisor(s) Scale” was used for the intern group. A modified version of the same scale was used for the supervisor group. The scale rated five domains: passive, evaluative, active, cooperative, and affective.

Results revealed that interns ranked perceptions of what supervisors considered important supervisory …

A Second Look At The Association Between Gender And Mortality On Antiretroviral Therapy, Serena P. Koenig, Alexandra Bornstein, Karine Severe, Elizabeth Fox, Jessy G. Devieux, Patrice Severe, Patrice Joseph, Adias Marcelin, Dgndy Alexandre Bright, Ngoc Pham, Pierre Cremieux, Jean William Pope

Department of Health Promotion and Disease Prevention

Objective

We assessed the association between gender and mortality on antiretroviral therapy (ART) using identical models with and without sex-specific categories for weight and hemoglobin.

Design

Cohort study of adult patients on ART. Setting GHESKIO Clinic in Port-au-Prince, Haiti.

Participants

4,717 ART-naïve adult patients consecutively enrolled on ART at GHESKIO from 2003 to 2008.

Main Outcome Measure

Mortality on ART; multivariable analyses were conducted with and without sex-specific categories for weight and hemoglobin.

Results

In Haiti, male gender was associated with mortality (OR 1.61; 95% CI: 1.30–2.00) in multivariable analyses with hemoglobin and weight included as control variables, but not …

Association Between Childhood Sexual Abuse And Hiv-Related Risk Factors For Hiv-Positive Haitian Women, Marie Sandra Severe

FIU Electronic Theses and Dissertations

Childhood sexual abuse (CSA) is one of the least studied HIV-related risk factors in Haiti although research in the United States and Europe has clearly established the link between childhood trauma and HIV risk behaviors. Understanding the role and impact of CSA on HIV-positive Haitian women is likely to strengthen future HIV prevention and treatment efforts aimed at this vulnerable group.

The current study was a cross-sectional examination of baseline data collected during a randomized trial of a cognitive-behavioral stress management (CBSM) intervention in Haiti. The purpose of this study was to analyze the association between CSA and sexual risk …

Organometallic Rhenium Dyes For Nitric Oxide Detection And Imaging, Lissette I. Lozano-Lewis

FIU Electronic Theses and Dissertations

The importance of sensing Nitric Oxide (NO) in physiology and medicine has led us to explore the reactivity of NO with organometallic Re dyes. Rhenium complexes were synthesized with the ability to react with NO and sense it under physiological conditions. Fluorescent 1,10-phenantroline complexes (phen)Re(PPh₃)(CO)₂OSO₂CF₃ (1) and (phen)Re(CH₃CN)(CO)₂OSO₂CF₃ (3) can sense NO in the range of 10 - 150 mM showing a decrease in fluorescence response at 514 nm and 532 nm respectively, upon NO-donor addition (l_exc = 360 nm). (phen)Re(THF)(CO)₂OSO₂CF …

Exploring Parental Perceptions Of Self-Efficacy, Role Modeling And Factors Contributing To Family Health Practices From An Employer-Provided Family Weight Management Program: A Mixed Methods Study, Kurt E. Vargo

FIU Electronic Theses and Dissertations

Parents provide a social learning environment where family nutrition, eating habits and physical activity are largely influenced by and correlated with parental modeling of these behaviors. Increasing self-efficacy is an important component in parents being role models because theoretically, it promotes cognitive change that supports their confidence and ability to modify behaviors that contributes to healthier family practices and biometric outcomes.

Phase one of this sequential two-phase study used biometric data (body mass index [BMI], cholesterol, glucose, and blood pressure) from parents (N = 37) participating in their employer’s family wellness initiative as dependent variables. Parental perceptions of nutrition, …

The Effect Of Vitamin D Supplementation On Cardiovascular Risk Factors Among Minorities With Type 2 Diabetes, Joel Exebio

FIU Electronic Theses and Dissertations

The aim of the present study was to determine the effect of vitamin D supplementation (4000 IU or 6000 IU of cholecalciferol daily for 6 months) on fasting plasma glucose, fasting insulin, glycated hemoglobin, and lipid profile in a sample of African-Americans and Hispanics with T2D and vitamin D insufficiency. Seventy five participants were recruited by community outreach. Plasma glucose concentration was measured by hexokinase enzymatic method. Glycated hemoglobin was measured by the DCA2000+ system. Insulin in fasting blood was determined by radioimmunoassay. Plasma total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein cholesterol were assayed by enzymatic methods. Serum vitamin …

Correlates Of Vitamin D Status In Healthy Older Adults Living In Miami-Dade And Its Association With Non-Skeletal Outcomes: A Cross-Sectional Study, Johanna Lopez

FIU Electronic Theses and Dissertations

Examining factors that affect vitamin D status in the fast-growing elderly population of Miami-Dade, Florida, is needed. Vitamin D deficiency in older adults has been linked to correlates of disability, including falls and fractures, and cardiovascular disease. The purpose of this study was to determine the proportion of vitamin D insufficient individuals and their relationship with vitamin D insufficiency in older adults (n=97) living in Miami-Dade. We evaluated the association between vitamin D status and 1) dual task physical performance to understand the link between vitamin D and cognition in the context of mobility; and 2) cardiometabolic risk, measured by …

Determinants And Disparities Of Survival In Triple-Negative Breast Cancer Patients: A Population-Based Retrospective Longitudinal Cohort Design Utilizing The Cox Proportional Hazard Analytical Model, Michael C. Belcon

FIU Electronic Theses and Dissertations

A significant racial disparity in breast cancer mortality exists among women in the United States. Triple-negative breast cancer (TNBC) is a breast cancer phenotype that may explain, in part, this disparity between white and African American women. The objective of this study was to determine the predictors of survival in TNBC and non-triple-negative breast cancer (NTNBC) patients.

Data on 168,756 female patients with a diagnosis of invasive breast cancer in the Surveillance Epidemiology and End Results (SEER) program were stratified based on breast cancer receptor phenotypes in this retrospective longitudinal cohort study design. Multiple logistic regressions were used for exploring …

In Search Of Safety, Negotiating Everyday Forms Of Risk: Sex Work, Criminalization, And Hiv/Aids In The Slums Of Kampala, Serena Cruz

FIU Electronic Theses and Dissertations

This dissertation offers an in-depth descriptive account of how women manage daily risks associated with sex work, criminalization, and HIV/AIDS. Primary data collection took place within two slums in Kampala, Uganda over the course of fourteen months. The emphasis was on ethnographic methodologies involving participant observation and informal and unstructured interviewing. Insights then informed document analysis of international and national policies concerning HIV prevention and treatment strategies in the context of Uganda. The dissertation finds social networks and social capital provide the basis for community formation in the sex trade. It holds that these interpersonal processes are necessary components for …

R Ates And Factors Associated With Falls In Older European Americans, Afro-Caribbeans, African-Americans, And Hispanics, Edgar Ramos Vieira, Ruth Tappen, Gabriella Engstrom, Bruno R. Da Costa

Department of Physical Therapy Faculty Publications

Purpose: To evaluate rates and factors associated with older adult falls in different ethnic groups. Participants and methods: Information on demographics, medical and falls history, and pain and physical activity levels was collected from 550 community-dwelling older adults (75±9 years old, 222 European Americans, 109 Afro-Caribbeans, 106 African-Americans, and 113 Hispanics). Results: Taking medications for anxiety (risk ratio [RR] =1.4, 95% confidence interval [CI] =1.1–2.0), having incontinence (RR =1.4, 95% CI =1.1–1.8, P=0.013), back pain (RR =1.4, 95% CI =1.0–1.8), feet swelling (RR =1.3, 95% CI =1.1–1.7), and age $75 years (RR =1.3, 95% CI =1.0–1.6) were associated with falls. …

Integrated Bioinformatics, Environmental Epidemiologic And Genomic Approaches To Identify Environmental And Molecular Links Between Endometriosis And Breast Cancer, Deodutta Roy, Marisa L. Morgan, Changwon Yoo, Alok Deoraj, Sandhya Roy, Vijay Kumar Yadav, Mohannad Garoub, Hamza Assaggaf, Mayur Doke

Department of Biostatistics Faculty Publications

We present a combined environmental epidemiologic, genomic, and bioinformatics approach to identify: exposure of environmental chemicals with estrogenic activity; epidemiologic association between endocrine disrupting chemical (EDC) and health effects, such as, breast cancer or endometriosis; and gene-EDC interactions and disease associations. Human exposure measurement and modeling confirmed estrogenic activity of three selected class of environmental chemicals, polychlorinated biphenyls (PCBs), bisphenols (BPs), and phthalates. Meta-analysis showed that PCBs exposure, not Bisphenol A (BPA) and phthalates, increased the summary odds ratio for breast cancer and endometriosis. Bioinformatics analysis of gene-EDC interactions and disease associations identified several hundred genes that were altered by …

Structural Basis For Suppression Of Hypernegative Dna Supercoiling By E. Coli Topoisomerase I, Kemin Tan, Qingxuan Zhou, Bokun Cheng, Zhongtao Zhang, Andrzej Joachimiak, Yuk-Ching Tse-Dinh

Biomolecular Sciences Institute: Faculty Publications

Escherichia coli topoisomerase I has an essential function in preventing hypernegative supercoiling of DNA. A full length structure of E. coli topoisomerase I reported here shows how the C-terminal domains bind single-stranded DNA (ssDNA) to recognize the accumulation of negative supercoils in duplex DNA. These C-terminal domains of E. coli topoisomerase I are known to interact with RNA polymerase, and two flexible linkers within the C-terminal domains may assist in the movement of the ssDNA for the rapid removal of transcription driven negative supercoils. The structure has also unveiled for the first time how the 4-Cys zinc ribbon domain and …

Mucociliary Dysfunction In Hiv And Smoked Substance Abuse, Srinivasan Chinnapaiyan, Hoshang J. Unwalla

HWCOM Faculty Publications

Impaired mucociliary clearance (MCC) is a hallmark of acquired chronic airway diseases like chronic bronchitis associated with chronic obstructive pulmonary disease (COPD) and asthma. This manifests as microbial colonization of the lung consequently leading to recurrent respiratory infections. People living with HIV demonstrate increased incidence of these chronic airway diseases. Bacterial pneumonia continues to be an important comorbidity in people living with HIV even though anti-retroviral therapy has succeeded in restoring CD4+ cell counts. People living with HIV demonstrate increased microbial colonization of the lower airways. The microbial flora is similar to that observed in diseases like cystic fibrosis and …

Sex-Positive Curricula: An Investigation Of The Relationship Between Physical Fitness, Self-Concept And Sexual Functioning, Lia Jiannine

FIU Electronic Theses and Dissertations

Despite the well-recognized benefits of exercise, Americans are gaining weight in astounding proportions and levels of physical activity are on the decline. The purpose of this study was to investigate a relationship between physical fitness, self-concept and sexual health. There is a dearth of knowledge on this relationship specifically in the context of sex-negative curricula, which is the dominate discourse in the United States.

One hundred and thirty-three participants between the ages of 18 - 50 volunteered for fitness testing and data collection. Physical fitness was assessed through body fat, resting metabolic rate, cardiovascular endurance, muscular strength, muscular endurance and …

Downregulation Of Host Tryptophan-Aspartate Containing Coat (Taco) Gene Restricts The Entry And Survival Of Leishmania Donovani In Human Macrophage Model, Venkateswara Reddy Gogulamudi, Mohan Lal Dubey, Deepak Kaul, Venkata Atluri

HWCOM Faculty Publications

Leishmania are obligate intracellular protozoan parasites of mammalian hosts. Promastigotes of Leishmania are internalized by macrophages and transformed into amastigotes in phagosomes, and replicate in phagolysosomes. Phagosomal maturation arrest is known to play a crucial role in the survival of pathogenic Leishmania within activated macrophages. Recently, tryptophan-aspartate containing coat (TACO) gene has been recognized as playing a central role in the survival of Mycobacterium tuberculosis within human macrophages by arresting the phagosome maturation process. We postulated that a similar association of TACO gene with phagosomes would prevent the vacuole from maturation in the case of Leishmania. In this study we …

Hiv Vaccine: Recent Advances, Current Roadblocks, And Future Directions, Muni Rubens, Venkataraghavan Ramamoorthy, Anshul Saxena, Nancy Shehadeh, Sandeep Appunni

Department of Health Promotion and Disease Prevention

HIV/AIDS is a leading cause of mortality and morbidity worldwide. In spite of successful interventions and treatment protocols, an HIV vaccine would be the ultimate prevention and control strategy. Ever since identification of HIV/AIDS, there have been meticulous efforts for vaccine development. The specific aim of this paper is to review recent vaccine efficacy trials and associated advancements and discuss the current challenges and future directions. Recombinant DNA technologies greatly facilitated development of many viral products which were later incorporated into vectors for effective vaccines. Over the years, a number of scientific approaches have gained popularity and include the induction …

Label-Free Surface-Enhanced Raman Spectroscopy-Linked Immunosensor Assay (Slisa) For Environmental Surveillance, Vinay Bhardwaj

FIU Electronic Theses and Dissertations

The contamination of the environment, accidental or intentional, in particular with chemical toxins such as industrial chemicals and chemical warfare agents has increased public fear. There is a critical requirement for the continuous detection of toxins present at very low levels in the environment. Indeed, some ultra-sensitive analytical techniques already exist, for example chromatography and mass spectroscopy, which are approved by the US Environmental Protection Agency for the detection of toxins. However, these techniques are limited to the detection of known toxins. Cellular expression of genomic and proteomic biomarkers in response to toxins allows monitoring of known as well as …