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Full-Text Articles in Medicine and Health Sciences
Influence Of Chondroitinase On Indirect Hemagglutination Titers And Phagocytosis Of Pasteurella Multocida Serogroups A, D And F, Richard B. Rimler, Karen B. Register, Tibor Magyar, Mark R. Ackermann
Influence Of Chondroitinase On Indirect Hemagglutination Titers And Phagocytosis Of Pasteurella Multocida Serogroups A, D And F, Richard B. Rimler, Karen B. Register, Tibor Magyar, Mark R. Ackermann
Mark R. Ackermann
Capsules of Pasteurella multocida serogroups A, D and F contain mucopolysaccharides which block antigenic determinants and prevent phagocytosis. In this study, capsules of serogroup A, D and F strains of P. multocida were depolymerized by enzyme treatment. Capsule depolymerization of serogroup D and F strains with chondroitinase increased indirect hemagglutination (IHA) test titers and enhanced phagocytosis by swine neutrophils. Capsule depolymerization of serogroup A strains with hyaluronidase increased IHA titers, but depolymerization with chondroitinase did not. When serogroup A strains were treated with a combination of chondroitinase and hyaluronidase, IHA test titers were lower than titers of the same strains …
Nonradioactive Colony Lift-Hybridization Assay For Detection Of Bordetella Bronchiseptica Infection In Swine, K. B. Register, Mark R. Ackermann, D. W. Dyer
Nonradioactive Colony Lift-Hybridization Assay For Detection Of Bordetella Bronchiseptica Infection In Swine, K. B. Register, Mark R. Ackermann, D. W. Dyer
Mark R. Ackermann
Current methods for the isolation and identification of Bordetella bronchiseptica from clinical samples are time-consuming and are based, in part, on subjective observations. We describe the use of a Bordetella-specific DNA probe in a nonradioactive colony lift-hybridization assay for the identification of B. bronchiseptica. Eleven of 82 clinical specimens were found to contain B. bronchiseptica by this method, while only 5 of these were reported to contain the organism when the specimens were analyzed by traditional methods. The chromosomal fragment containing a sequence complementary to the probe appeared to be conserved in B. bronchiseptica isolates from swine from a variety …
Influence Of Inoculation Route On The Carrier State Of Salmonella Choleraesuis In Swine, Jeffrey T. Gray, Paula J. Fedorka-Cray, Thomas J. Stabel, Mark R. Ackermann
Influence Of Inoculation Route On The Carrier State Of Salmonella Choleraesuis In Swine, Jeffrey T. Gray, Paula J. Fedorka-Cray, Thomas J. Stabel, Mark R. Ackermann
Mark R. Ackermann
This study was designed to investigate the carrier state of swine infected with Salmonella choleraesuis. Thirty-five pigs were divided into 3 groups. Groups 1 (n = 15) and 2 (n = 16) were challenged with 108 CFU of S. choleraesuis intranasally or by gastric route, respectively. Group 3 (n = 4) served as uninoculated controls. Pigs were necropsied at 2, 4, 6, and 12 weeks post inoculation. Clinical signs and microscopic lesions were more severe for group 1. Salmonella choleraesuis was recovered from a greater percentage of tissue samples for group 1 versus group 2 at 2, 4, and 6 …