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Full-Text Articles in Medicine and Health Sciences
Invasion Of Caco-2 Cells By Salmonella Typhimurium (Copenhagen) Isolates From Healthy And Sick Chickens, Theodore J. Kottom, Lisa K. Nolan, John Brown
Invasion Of Caco-2 Cells By Salmonella Typhimurium (Copenhagen) Isolates From Healthy And Sick Chickens, Theodore J. Kottom, Lisa K. Nolan, John Brown
Lisa K. Nolan
In a previous study, Salmonella isolates of sick birds were distinguished from those of apparently healthy birds by their high degree of invasion of tissue culture cells. In this study, a single pair of Salmonella isolates was examined to determine the source of this observed difference in invasion. When isolates were allowed to invade Caco-2 cells for 8 hours, the isolate from the sick bird (S) appeared to invade in greater numbers than did the isolate from the healthy bird (H). However, when invasion was distinguished from intracellular growth/survival, it was found that H invaded in greater numbers than S, …
Influence Of Chondroitinase On Indirect Hemagglutination Titers And Phagocytosis Of Pasteurella Multocida Serogroups A, D And F, Richard B. Rimler, Karen B. Register, Tibor Magyar, Mark R. Ackermann
Influence Of Chondroitinase On Indirect Hemagglutination Titers And Phagocytosis Of Pasteurella Multocida Serogroups A, D And F, Richard B. Rimler, Karen B. Register, Tibor Magyar, Mark R. Ackermann
Mark R. Ackermann
Capsules of Pasteurella multocida serogroups A, D and F contain mucopolysaccharides which block antigenic determinants and prevent phagocytosis. In this study, capsules of serogroup A, D and F strains of P. multocida were depolymerized by enzyme treatment. Capsule depolymerization of serogroup D and F strains with chondroitinase increased indirect hemagglutination (IHA) test titers and enhanced phagocytosis by swine neutrophils. Capsule depolymerization of serogroup A strains with hyaluronidase increased IHA titers, but depolymerization with chondroitinase did not. When serogroup A strains were treated with a combination of chondroitinase and hyaluronidase, IHA test titers were lower than titers of the same strains …
Nonradioactive Colony Lift-Hybridization Assay For Detection Of Bordetella Bronchiseptica Infection In Swine, K. B. Register, Mark R. Ackermann, D. W. Dyer
Nonradioactive Colony Lift-Hybridization Assay For Detection Of Bordetella Bronchiseptica Infection In Swine, K. B. Register, Mark R. Ackermann, D. W. Dyer
Mark R. Ackermann
Current methods for the isolation and identification of Bordetella bronchiseptica from clinical samples are time-consuming and are based, in part, on subjective observations. We describe the use of a Bordetella-specific DNA probe in a nonradioactive colony lift-hybridization assay for the identification of B. bronchiseptica. Eleven of 82 clinical specimens were found to contain B. bronchiseptica by this method, while only 5 of these were reported to contain the organism when the specimens were analyzed by traditional methods. The chromosomal fragment containing a sequence complementary to the probe appeared to be conserved in B. bronchiseptica isolates from swine from a variety …
Influence Of Inoculation Route On The Carrier State Of Salmonella Choleraesuis In Swine, Jeffrey T. Gray, Paula J. Fedorka-Cray, Thomas J. Stabel, Mark R. Ackermann
Influence Of Inoculation Route On The Carrier State Of Salmonella Choleraesuis In Swine, Jeffrey T. Gray, Paula J. Fedorka-Cray, Thomas J. Stabel, Mark R. Ackermann
Mark R. Ackermann
This study was designed to investigate the carrier state of swine infected with Salmonella choleraesuis. Thirty-five pigs were divided into 3 groups. Groups 1 (n = 15) and 2 (n = 16) were challenged with 108 CFU of S. choleraesuis intranasally or by gastric route, respectively. Group 3 (n = 4) served as uninoculated controls. Pigs were necropsied at 2, 4, 6, and 12 weeks post inoculation. Clinical signs and microscopic lesions were more severe for group 1. Salmonella choleraesuis was recovered from a greater percentage of tissue samples for group 1 versus group 2 at 2, 4, and 6 …
Thoracoabdominal Myelolipomas And Carcinoma In A Lovebird (Agapomis Sp.), James R. Andreasen Jr., Claire B. Andreasen, Kenneth S. Latimer, Jeri L.H. Oliphant
Thoracoabdominal Myelolipomas And Carcinoma In A Lovebird (Agapomis Sp.), James R. Andreasen Jr., Claire B. Andreasen, Kenneth S. Latimer, Jeri L.H. Oliphant
Claire B. Andreasen
Myelolipomas are uncommon benign tumors composed of mature fat cells and hematopoietic cells (myeloid, erythroid, and lymphoid) in various proportions.1,4 In humans, these neoplasms rarely cause symptoms unless they are unusually large or unless they hemorrhage. Myelolipomas have been found incidentally at postmortem examination; however, their antemortem diagnosis is becoming more common in humans with increased use of ultrasound and computed tomography. In contrast, myelolipomas are reported infrequently in animals. Carcinomas (malignant tumors of epithelial origin) are found in many species of animals. In this report, we describe 2 intraabdominal myelolipomas and a cranial thoracic carcinoma of undetermined origin in …
Gene Evolution Of Epoxide Hydrolases And Recommended Nomenclature, Jeffrey K. Beetham, David Grant, Michael Arand, Joan Garbarino, Tomohiro Kiyosue, Franck Pinot, Franz Oesch, William R. Belknap, Kazuo Shinosaki, Bruce D. Hammock
Gene Evolution Of Epoxide Hydrolases And Recommended Nomenclature, Jeffrey K. Beetham, David Grant, Michael Arand, Joan Garbarino, Tomohiro Kiyosue, Franck Pinot, Franz Oesch, William R. Belknap, Kazuo Shinosaki, Bruce D. Hammock
Jeffrey K. Beetham
We have analyzed amino acid sequence relationships among soluble and microsomal epoxide hydrolases, haloacid dehalogenases, and a haloalkane dehalogenase. The amino-terminal residues (1-229) of mammalian soluble epoxide hydrolase are homologous to a haloacid dehalogenase. The carboxy-terminal residues (230-554) of mammalian soluble epoxide hydrolase are homologous to haloalkane dehalogenase, to plant soluble epoxide hydrolase, and to microsomal epoxide hydrolase. The shared identity between the haloacid and haloalkane dehalogenases does not indicate relatedness between these two types of dehalogenases. The amino-terminal and carboxy-terminal homologies of mammalian soluble epoxide hydrolase to. the respective dehalogenases suggests that this epoxide hydrolase, but not the soluble …