Medicine and Health Sciences Commons^™

Clinical Characteristics, Antimicrobial Resistance And Treatment Outcomes Of Multidrug-Resistant Escherichia Coli Infection In Dogs And Cats At A Veterinary Teaching Hospital In Thailand, Jeerawat Soonthornsit, Sukanya Apiratwarrasakul, Nathita Phumthanakorn

The Thai Journal of Veterinary Medicine

This retrospective study investigated the clinical characteristics of multidrug-resistant (MDR) Escherichia coli and its antimicrobial resistance phenotypes and analyzed the treatment outcomes of MDR E. coli infection in dogs and cats. The medical data of dogs and cats diagnosed in 2020 with E. coli infection at a veterinary teaching hospital were analyzed. Of 94 cases, the frequency of MDR E. coli (66%) infection was higher than that of non-MDR E. coli (34%). MDR E. coli was significantly more frequently detected in female dogs than non-MDR E. coli (P < 0.026). The most frequent MDR E. coli isolation sites were the urinary tract in dogs and skin wounds in cats. MDR E. coli isolates from dogs were highly resistant to ampicillin (96.1%), enrofloxacin (80.4%) and tetracycline (78.4%). Resistance to ampicillin (100%), enrofloxacin (90.9%), marbofloxacin (72.7%) and tetracycline (72.7%) occurred frequently in MDR E. coli isolates from cats. Low resistance to amikacin was detected in the MDR E. coli isolates from dogs and cats. The rates of clinical cure and non-clinical cure of the MDR E. coli and non-MDR E. coli cases were not significantly different. The duration of antimicrobial treatment for MDR E. coli was significantly longer in cats (12.6 ± 5.85 days) than in non-MDR E. coli cases (7 ± 0 days) (P < 0.048). Adjunctive therapy was prescribed more frequently in MDR E. coli (20%) than non-MDR E. coli cases (8.3%). The presence of MDR E. coli was high in this study. High resistance to commonly used antimicrobial drugs and treatment complications was observed in this study.

Prevalence Of Shiga Toxin-Producing Escherichia Coli In Buffaloes On Smallholdings In Coastal Area In Bangladesh, Md Shahriar Mannan, Farzana Rabbi, Md Gholam Kibria, Sirazul Islam, Md. Bayzid, Zamila Bueaza Bupasha, Md Zohorul Islam, Md Samun Sarker, Paritosh Kumar Biswas

The Thai Journal of Veterinary Medicine

Shiga toxin-producing Escherichia coli (STEC) is an important causal agent of food borne diseases. Although the presence of STEC has been reported in cattle, goat, and sheep in previous studies in Bangladesh, there is seemingly no information on sorbitol non-fermenting STEC (SN-F STEC) carriage in buffalo. We aimed to assess the prevalence of SN-F STEC and to identify common virulence genes in SN-F STEC strains from apparently healthy buffaloes on smallholdings in coastal area in Bangladesh. Total 308 buffaloes on smallholdings in Patenga and Anwara Upazila, Chattogram division, Bangladesh were randomly sampled by collecting a swab from the recto-anal junction …

Prevalence And Multidrug Resistance Pattern Of E. Coli Among Urinary Tract Infection Patients In Tertiary Care Hospital Of Multan, Hubza Ruatt Khan, Mehvish Javeed, Asghar Javed, Nisma Farooq

Journal of Bioresource Management

Urinary Tract Infection is alarming problem worldwide due to the intensity of antimicrobial resistance. Escherichia coli is the most predominant organism in UTI. This study was planned to evaluate demographic parameters, the prevalence of E. coli, and antimicrobial resistance patterns among E. coli isolates from UTI patients in Nishtar Hospital of Multan from January to June 2018. A total of 350 mid-stream urine samples were collected from different patients having age group from 25 to 60 years and processed by standard laboratory procedures. Out of 350 samples, 100 samples were observed as critical bacteremia. Escherichia coli and Klebsiella …

Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants, Batrisyia Syazana Faridon, Mohd Zamri-Saad, Annas Salleh

The Thai Journal of Veterinary Medicine

Sepsis is an important disease in both humans and animals. In animals, it is usually caused by septicaemia after infection and has not been well-studied. This article describes a retrospective analysis of sepsis cases in small and large ruminants submitted to the Post-Mortem Laboratory, Faculty of Veterinary Medicine, Universiti Putra Malaysia between the years 2007 and 2017. The incidence of sepsis was found to be at 40.5% in small ruminants and 38.6% in large ruminants. In both small and large ruminants, sepsis was mainly caused by bacterial infection, which was significantly (p<0.05) higher compared to parasitic and viral infections. Most cases of sepsis occur due to infections originating from the gastrointestinal tract and this is followed by the respiratory tract. Escherichia coli has been found to be the most common bacterial pathogen of sepsis in ruminants; significantly (p<0.05) higher compared to other bacterial pathogens like Klebsiella pneumoniae, Pasteurella sp., and Staphylococcus aureus. Sepsis involving E. coli has often resulted in pure isolation of the pathogen, thus being suggestive of a single infection. Underlying or concurrent infection is the most important risk factor leading to sepsis in ruminants, with significant difference (p<0.05) compared to other risk factors such as stress or malnutrition. Associations between the gastrointestinal system as the point of entry and sepsis caused by either E. coli or K. pneumoniae have been noted. This study provides an insight into a basic understanding of sepsis in ruminants.

Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A total of 292 E. coli isolates obtained from fecal samples from pigs in Central (n = 103) and Northeastern (n = 189) provinces of Thailand were included in this study. Eighty-six E. coli isolates were phenotypically confirmed to be β- lactamases producers and screened for the presence of β-lactamase genes. The CTX-M family was most frequently identified (90.7%). The blaCTX-M-15 gene belonging to CTX-M group I (59.3%) was the most predominantly identified CTX-M genotype followed by blaCTX-M-14 (31.4%) and blaCTX-M-4 (25.6%). The blaTEM-1 gene was prevalent (75.6%). The blaCTX-M-4 and blaCTX-M-14 genes were located on conjugative plasmid. The results …

Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan

The Thai Journal of Veterinary Medicine

Escherichia coli is a primary reservoir of antimicrobial resistance, known chiefly for the container of AMR-encoding genes (ARGs), and poses potential risks to human and animal health. This study investigated AMR phenotypes and ARGs in 90 E. coli isolates from different pig groups in 10 farms in southern Vietnam. The minimum inhibitory concentration (MIC) of 19 common antimicrobial agents was determined, and polymerase chain reaction (PCR) was used to investigate seven ARGs (blaTEM, aadA1, strA, dfrA12, sul3, cmlA and tetA). Cohen’s kappa statistic (κ) was applied to assess the concordance between phenotypic and genotypic profiles. A total of 81.1% of …

Surveillance Of Multidrug-Resistant Uropathogenic Escherichia Coli In Hospitalized Patients And Community Settings In The South Of Lebanon, Nourhan Darwich, Ali Samaha, Hanan Al Nuqaidan, Ahmad Tassi, Mirna Fawaz

BAU Journal - Health and Wellbeing

Urinary Tract Infection (UTI) is one of the common infectious diseases in both hospitals as well as community settings; they are recognized to be among the most serious worldwide bacterial infections impacting 150 million people globally every year. The purpose of this study was to assess the changing antibiotics resistance profile for uropathogenic Escherichia coli isolated from community and hospital setting over a period of time (2018–2019) with a special emphasis on ESBL/MDR producing Escherichia coli. A descriptive retrospective study was conducted among patients with uropathogenic Escherichia coli from both community and hospital settings in south Lebanon. Out of 863 …

Sepsis Por Escherichia Coli De Inicio Infrecuente En Paciente Con Linfoma De Células T Y Nk, José Manuel Vela Ruiz, Carlos Noriega Baella, Eliana Valera Vásquez, Ilan Vargas Torres

Revista de la Facultad de Medicina Humana

La sepsis se da en presencia de un foco infeccioso; en este caso por Escherichia coli (E.coli), las cuales poseen toxinas que pueden causar daños severos en la funcionalidad y morfología del organismo, viéndose empeorado en pacientes con enfermedades que afecten al sistema inmunitario; como es este caso en presencia de neoplasia hematológica. Paciente femenina de 42 años , con diagnóstico de Linfoma no Hodgkin de células T-NK presenta una placa necrótica en el paladar, temperatura por encima de 38°C, frecuencia respiratoria superando las 20 respiraciones por minutos y frecuencia cardíaca superando los 90 latidos por minuto, al examen de …

Comorbilidades Asociadas A Infección De Tracto Urinario Por Escherichia Coli Blee Positivo Del Hospital Vitarte. 2017 - 2018, Yanina Chipa-Paucar

Revista de la Facultad de Medicina Humana

Introducción:as infecciones por bacterias productoras de β-lactamasas de espectro extendido (BLEE) son un serio problema en nuestro país desde principios del año 2000 se ha dado un aumento progresivo en la frecuencia de las infecciones causadas por productoras de β-lactamasas de espectro extendido (BLEE). Objetivo: Determinar las comorbilidades asociadas a pacientes con Infección del Tracto Urinario causada por E. coli BLEE del servicio de medicina interna en el Hospital de Vitarte en el periodo 2017-2018. Métodos: Estudio observacional, analítico de tipo casos y controles. Se contó con una muestra correspondiente a 114 divididos en 57 casos y 57 controles. Se …

Effect Of Cuprum Metallicum Potentised Through Both Serial Dilution And Succussion In Comparison To Succussion Alone On Escherichia Coli Bacterial System And Electrical Properties Of Poly (Vinylidene Fluoride-Co-Hexafluoropropylene) Polymer, A Gayen, Mondal D, D Bera, P Biswas, B Paul, D Bhar, S Das, R Narula, A Khurana

Indian Journal of Research in Homoeopathy

Background: Homoeopathic medicines are traditionally potentized by serial dilution followed by succussion. Respective roles of these two components need to be assessed and explored for which the present study was undertaken. Objective: To compare the effect of the medicine Cuprum metallicum (Cup. met.) potentised through both serial dilution and succussion with succussion alone on selected biological and physical systems. Method: Starting with the medicine Cup. met. at 6C, we potentized it further to 30C and 200 C by serial dilution, followed by succussion (Set A). The same medicine at 6C was also potentized to 30C and 200C by using succussion …

The Antimicrobial Effects Of Allium Sativum On Escherichia Coli, David Borja, Johnny Aldan, Bsn, Rn

Journal of Health Disparities Research and Practice

Allium sativum (Garlic) is an herb that is grown all over the world, and can be found in the homes of many people. This herb has many medicinal properties, but is mainly known for its antimicrobial properties. Fresh garlic contains enzymes called “alliinase” and “alliin”. When garlic is crushed or cut into the alliinase converts the alliin into allicin. Allicin is what gives the garlic its antimicrobial properties and odor.

The antimicrobial activity of the Allium sativum extract was tested on Escherichia coli, a gram-negative bacteria, using the agar-well diffusion method. A test tube containing only Escherichia coli was …

Virulence Factors And Antimicrobial Resistance In Uropathogenic Escherichiacoli Strains Isolated From Cystitis And Pyelonephritis, Hosseinali Ghazvini, Keyvan Taheri, Elahe Edalati, Ali Miri, Mansour Sedighi, Shiva Mirkalantari

Turkish Journal of Medical Sciences

Background/aim: The aim of this study was to investigate the prevalence of virulence genes as well as patterns of antibiotic resistance in cystitis and pyelonephritis uropathogenic Escherichia coli (UPEC) isolates. Materials and methods: Two hundred UPEC isolates were collected from hospitalized patients with pyelonephritis (n = 50) and cystitis (n = 150) in Shafa Hospital in Iran. Antimicrobial susceptibility and ESBL production were determined with confirmatory tests. Polymerase chain reaction assay was performed to determine the prevalence of virulence genes in UPEC strains. Results: Of a total 200 UPEC isolates, the highest and lowest resistance rates to antibiotics were for …

A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi

Turkish Journal of Veterinary & Animal Sciences

Avian colibacillosis (AC) is frequently reported from poultry farms in the west of Iran. The aim of the present survey was to evaluate the phylogeny of Escherichia coli isolated from AC through the Clermont triplex protocol and gyrA gene sequencing. One hundred and fifty fresh carcasses suspected of having died from colibacillosis were sampled. The specimens were examined for E. coli isolation and identification. The extracted DNA repertoire of the isolates was subjected to phylogenetic analysis using Clermont triplex PCR. A DNA fragment of 1.8 kbp of the gyrA gene was amplified from a representative of each phylogroup or subgroup …

Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi

Turkish Journal of Veterinary & Animal Sciences

The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance (AMR) patterns of the isolates and evaluate them for the presence of extended-spectrum ß-lactamase (ESBL) using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep (n = 96) and goats (n = 08). The samples were processed and used for bacterial isolation. E. coli isolates (n = 58) including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and …

Biofilm Formation In Uropathogenic Escherichia Coli: Association With Adhesion Factor Genes, Hojjatolah Zamani, Ali Salehzadeh

Turkish Journal of Medical Sciences

Background/aim: Biofilm formation is a major determinant factor in the development of urinary tract infections (UTIs) by uropathogenic Escherichia coli (UPEC). Several adhesion factors are involved in attachment of bacterial cells to the urinary tract and biofilm development. Materials and methods: The possible relationship between different adhesion factor genes (AFGs) and biofilm formation among UPEC isolates was investigated. Prevalence of different AFGs including fimA, fimH, papAH, papC, papEF, sfa-S, foc/G, and bmaE and their association to biofilm formation were investigated. Phenotypic expression of type 1 and P fimbriae was also investigated. Results: Our results showed that 84% of UPEC were …

Fecal Carriage Of Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli And Klebsiella Spp. In A Turkish Community, Derya Çakir Erdoğan, Füsun Cömert, Eli̇f Aktaş Sepetci̇, Fürüzan Köktürk, Canan Külah

Turkish Journal of Medical Sciences

Background/aim: The aim of this study was to determine the prevalence of fecal carriage of extended-spectrum beta-lactamase (ESBL)-producing bacteria, enzyme types, and risk factors affecting colonization. Materials and methods: A total of 576 stool samples from outpatients were examined between October 2012 and May 2013. Screening was done with selective EMB plates. ESBL were detected by double-disk synergy and confirmed agar strip gradient methods. Enzyme types were determined by PCR. Results: The prevalence of fecal carriage was found as 30% (173 of 576). Recent use of antibiotics, hospitalization and surgical operation, diabetes, crowded household populations, and old age were associated …

Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic …

Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …

Characterization Of The Role That Alternative Ribonucleotide Reductases Play In Restoring Replication In The Presence Of Hydroxyurea In Escherichia Coli, Michael Sadek

PSU McNair Scholars Online Journal

DNA replication is essential for cells to grow and divide. Ribonucleotide reductase is an essential enzyme that is responsible for the formation of deoxyribonucleotides that are used in DNA synthesis during replication. Hydroxyurea is a chemotherapeutic agent that is thought to work by specifically inhibiting the ribonuceotide reductase to prevent replication. However, recent studies in E. coli have shown that following an initial period of inhibition, DNA synthesis then recovers in the presence of hydroxyurea, suggesting that the mode of death and cellular response to hydroxyurea is more complex than originally proposed. The E.coli genome encodes three ribonucleotide reductases that …

Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano

Turkish Journal of Veterinary & Animal Sciences

Pharmacokinetics (PK), pharmacodynamics (PD), and PK/PD ratios of a new solvate of enrofloxacin (enrofloxacin hydrochloride-dihydrate; enro-C), were studied in hamsters. Enrofloxacin from Baytril1397904493 5% (enroR) served as the reference preparation. Two groups of 60 Syrian golden hamsters were intramuscularly injected individually with 10 mg/kg of enroR or with enro-C. Tissue and serum samples were obtained for 72 h; enrofloxacin concentrations were determined by HPLC with UV detection. Ninety percent minimum inhibitory concentrations (MIC90) were determined for strains of methicillin-resistant Staphylococcus aureus, Leptospira interrogans, and Escherichia coli. All PK variables were statistically different between groups (P < 0.01). CMAX of enrofloxacin for enro-C was 17.3 μg/mL and it was 2.6 μg/mL for enroR. AUC was considerably higher for enro-C (459.2 μg/mL h vs. 19.9 μg/mL h). There were no statistically significant differences in MIC90 values between enroR and enro-C. Tissue concentrations of enro-C in all cases were higher and remained above the MIC for longer periods than those of enroR. Relevant PK/PD ratios for enrofloxacin (AUC/MIC ? 125 and CMAX > MIC = 10-12) are consequently …

Prevalence Of Quinolones Resistance Among Patients With Urinary Tract Infection At Menou, Amira Abd El-Kader El-Hendy, Rasha Rashed Mohamed, Samia Hassan Kandel

Menoufia Medical Journal

Objective The aim of the study was to examine the prevalence of quinolone resistance and determine appropriate empiric antibiotics for patients with urinary tract infection (UTI). Background UTI is the most common disease of the urinary tract and it is a major cause of morbidity in both hospitals and communities. Most infections arise from Escherichia coli. Quinolones are effective for treatment of UTIs. Therapy against infectious diseases with antimicrobial agents such as quinolones is very dynamic, with high probability for bacterial resistance. Patients and methods This study was carried out on 150 patients (80 patients suffering from UTI with …

Prevalence Of Salmonella Enterica, Escherichia Coli And Staphylococcus Aureus In Raw Meat In Thai Self-Service Style Restaurants In Khon Kaen Municipality, Sunpetch Angkititrakul, Arunee Polpakdee, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

One hundred and fifty eight raw meat samples were collected from 33 Thai self-service style restaurants in Khon Kaen municipality during July-October 2011. The fresh meat included pork, beef and chicken and was provided for self-grilling or boiling. All the samples were examined for contamination of three major foodborne pathogens including Salmonella enterica, Escherichia coli and Staphylococcus aureus using standard protocol. High prevalence of S. enterica (44%), E. coli (60%) and S. aureus (25%) was observed. Seventy-seven percent of the samples were positive for at least one of the three pathogens, while only 8% were found to carry all three …

Effectiveness Of A Portable, Large-Area Ultraviolet Germicidal Device, Mark P. Buttner, Thomas Yee, Patricia Cruz, Vanessa Stevens

Nevada Journal of Public Health

Effective disinfection of the hospital environment is a key component in the prevention of healthcare-associated infections. The objective of this project was to evaluate the effectiveness of an ultraviolet germicidal device in reducing the concentrations of culturable bacteria on indoor surfaces. The ultraviolet germicidal device was installed and operated in four experimental trials conducted in a microbiology research chamber. Agar plates inoculated with known concentrations of two test microorganisms were placed on benches inside the chamber at two distances, 1.5 meters and 3.0 meters from the machine, for exposure times of 5 minutes, 10 minutes, and 20 minutes. With test …

Effect Of Melatonin On Contractile Activity In Intraurethrally Instilled E. Coli-Induced Acute Prostatitis Rat Model, Bakhtiyar Huseynov, Nadi̇re Eser, İbrahi̇m Ati̇lla Aridoğan, Şeyda Erdoğan, Yusuf Kenan Dağlioğlu, Hacer Si̇nem Büyüknacar, Fati̇h Köksal, Cemi̇l Göçmen

Turkish Journal of Medical Sciences

To investigate whether prostatic inflammation affects the contractile responses of prostate tissue and then whether melatonin has any restorative effect on the contractile responses in a rat model of bacterial prostatitis at early stages. Materials and methods: We evaluated the nerve-evoked, adrenergic agonist-induced or cholinergic agonist-induced contractions of isolated prostate tissue at 24 and 72 h after intraurethrally instilled Escherichia coli-treatment. We also analyzed the histological changes in the prostate. Secondarily, we investigated the effect of melatonin pretreatment (7 days, 10 mg kg^{-1} day^{-1}) on the contractile responses and the histopathologic changes. Results: The degrees of acute inflammatory cell infiltration, …

Horner's Syndrome Associated With Escherichia Coli Infection In A Raccoon (Procyon Lotor) - A Case Report, Fernando Nájera Muñoz, Alfredo Carlos Suárez Saavedra

The Thai Journal of Veterinary Medicine

This clinical case describes a three-year-old male raccoon (Procyon lotor), castrated, from La Lajita Oasis ParkZoological Park (Spain) which showed lack of appetite, state of mental depression and mild dehydration. Duringclinical examination an abscess was detected in the left ventrolateral area of the neck as well as clinical signscompatible with Horner’s Syndrome in the left eye. A hypersensitivity test was carried out by sympatheticinnervation with topical epinephrine 0.0001% and results suggested the presence of second-order Horner’s Syndromeformed by pressure from the abscess on the sympathetic innervation of the left eye. A radiologic study of the neckwas performed and blood and …

Plasmid Profiles Of Multidrug-Resistant Escherichia Coli From Clinically Healthy Swine, Khin Khin Lay, Nisit Chansong, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A total of 145 Escherichia coli isolates originated from clinically-healthy pigs were analyzed for plasmidprofiles. All but one isolate (n=144) carried one to nine plasmids with size ranging from 0.7-16.2 Mda. Fifty-sixplasmid profiles were defined based on sizes and numbers of plasmids in each strain, of which the most commonpatterns were 15.8, 4, 3.3, 1.7, 1.3 Mda and 16.2, 15.8, 4, 3.3, 2.3, 1.7, 1.3, 1, 0.7 Mda. The results indicate widedistribution of plasmids among the commensal E. coli isolates originated from clinically-healthy pigs.

Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old Chios ewe was submitted to the Clinic of Farm Animals (School of Veterinary Medicine, Aristotle University of Thessaloniki). The animal had delivered 2 healthy lambs, 2 days earlier, and was inappetent, depressed, and reluctant to move, while it had tachycardia and tachypnea. The cervix was opened at a dilation of 1 finger in diameter and fetid fetal membranes expelled from the vagina. Serum biochemistry revealed renal and liver disease, while ultrasonography revealed uterine dilatation. Laparotomy revealed a partly autolysed embryo in the uterus and gangrenous metritis. Clostridium perfringens and Escherichia coli were isolated in bacterial cultures from the …

Histopathological Effects Of S. Xylosus Peptidoglycan In Comparison To E. Coli Lipopolysaccharide In The Urinary Tract Of Mice, Harith Jabbar Fahad Al-Mathkhury, May Talib Flaih, Hiba Khalil Tawfiq

Turkish Journal of Medical Sciences

The present work, among other previous studies done in our lab, aimed to highlight the histopathological effect of S. xylosus peptidoglycan in comparison to LPS of E. coli. Materials and methods: One hundred and fifty urine specimens were collected from urinary tract infection patients visiting Baghdad hospitals. The histopathological effects of S. xylosus S24 peptidoglycan was studied in the urinary tract of female mice by injecting 5 animal groups at the following concentrations: 1000, 2000, 3000, 4000, and 5000 µg/mL. Another 5 groups were injected with 10, 25, 50, 75, and 100 ng/mL of E. coli (serotype 0128:B12) LPS. Results: …

Kontaminasi Bakteri Escherichia Coli Pada Makanan Pedagang Kaki Lima Di Sepanjang Jalan Margonda Depok, Jawa Barat, Dewi Susanna, Yvonne M. Indrawani, Zakianis Zakianis

Kesmas

Menteri Kesehatan menetapkan bahwa makanan dan minuman tidak boleh mengandung bakteri Escherichia coli (E. coli). Namun, kebanyakan pemerintah daerah tidak menindaklanjutinya dengan menerapkan peraturan yang lebih teknis untuk mencegah penyakit-penyakit yang ditularkan lewat makanan. Penelitian ini bertujuan untuk mengukur kontaminasi E. coli dalam makanan yang dijual oleh pedagang kaki lima (PKL). Seratus PKL di sepanjang Jalan Margonda Kota Depok, Jawa Barat, dipilih secara acak sebagai sampel. Sebanyak 100 PKL, E. coli pada sampel berbagai jenis makanan diukur dengan metode most probable number, sementara sanitasi PKL dan kehigienisan penjamah makanan diamati. Ditemukan secara umum bahwa air bersih yang digunakan untuk memasak, …

Prevalence Of The Cellular And Molecular Antimicrobial Resistance Against E. Coli Isolated From Thai Broilers, Sarawoot Mooljuntee, Piyarat Chansiripornchai, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The antimicrobial resistant characteristics both of the cellular and molecular levels of Escherichia coli in broilers were performed by disk diffusion test and polymerase chain reaction technique. Specific primers for the aadA, aac(3)-IV, cmlA, cat1, tetA, sul1, SHV, CITM, ereA and dhfrV gene were used to detect resistance to gentamicin, chloramphenicol, tetracycline, cephalothin, ampicillin, erythromycin, sulfonamide+trimetroprim. The 30 samples of E. coli were found to be 100% antimicrobial disk resistant to tetracycline, ampicillin and erythromycin and revealed a 90%, 93.3% and 73.3% resistant gene to tetA, CITM and ereA, respectively. While resistance to 73.3% cephalothin and 26.7% sulfonamide+trimethoprim was revealed …