Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Medicine and Health Sciences
First Detection And Molecular Identification Of Babesia Gibsoni In Two Dogs From The Aydın Province Of Turkey, Nuran Aysul, Kerem Ural, Bülent Ulutaş, Hasan Eren, Tüli̇n Karagenç
First Detection And Molecular Identification Of Babesia Gibsoni In Two Dogs From The Aydın Province Of Turkey, Nuran Aysul, Kerem Ural, Bülent Ulutaş, Hasan Eren, Tüli̇n Karagenç
Turkish Journal of Veterinary & Animal Sciences
Small (1-3 µm in diameter, ring, oval, or comma shaped) piroplasms suggestive of Babesia spp. were observed upon microscopic examination of Giemsa-stained peripheral blood smears from a 3.5-year-old American Pit Bull Terrier with clinical signs of pyrexia, weakness, mucous membrane pallor, and depression living in Aydın, Turkey, in February, 2009. To confirm the presence and to identify the species of Babesia spp., a polymerase chain reaction (PCR) technique was performed using primers amplifying a 670 bp fragment of the 18S rRNA gene of Babesia gibsoni and the PCR products were analyzed by sequencing. The nucleotide sequence was compared to the …
Clostridium Botulinum In Honey: Prevalence And Antibiotic Susceptibility Of Isolated Strains, Ahmet Koluman, Berfi̇n Meli̇koğlu Gölcü, Okan Deri̇n, Si̇bel Özkök, Fabrizio Anniballi
Clostridium Botulinum In Honey: Prevalence And Antibiotic Susceptibility Of Isolated Strains, Ahmet Koluman, Berfi̇n Meli̇koğlu Gölcü, Okan Deri̇n, Si̇bel Özkök, Fabrizio Anniballi
Turkish Journal of Veterinary & Animal Sciences
Clostridium botulinum and rare strains of C. butyricum and C. baratii produce an extremely potent toxin, the botulinum neurotoxin (BoNT). Infant botulism is significant for both its high mortality rates and for the sophisticated treatment that it requires. Isolation and identification of C. botulinum according to standards depend on mouse bioassays to determine toxin-producing ability of strains. Polymerase chain reaction (PCR) is used to detect the types of toxins expressed by bacteria. Since honey is an important source of infant botulism, we determined the types of toxins secreted by C. botulinum strains and their antibiotic resistance. In this study, the …
Indel Mutation Of The Add1/Srebp-1c Gene In The South Anatolian Red And East Anatolian Red Cattle Breeds, Kemal Özdem Öztabak, Iraz Akiş Akad, Feraye Esen Gürsel, Ati̇la Ateş, Hasret Yardi̇bi̇, Gülhan Türkay Hoştürk
Indel Mutation Of The Add1/Srebp-1c Gene In The South Anatolian Red And East Anatolian Red Cattle Breeds, Kemal Özdem Öztabak, Iraz Akiş Akad, Feraye Esen Gürsel, Ati̇la Ateş, Hasret Yardi̇bi̇, Gülhan Türkay Hoştürk
Turkish Journal of Veterinary & Animal Sciences
The present study was designed to determine genotypes of adipocyte determination and differentiation-dependent factor 1 (ADD1)/sterol regulatory element-binding protein-1c (SREBP1c) in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle. Fifty cattle from the SAR breed and 43 cattle from the EAR breed were used. Intron 7 of the ADD1/SREBP1c gene containing an 84 bp indel mutation was amplified by polymerase chain reaction (PCR). The number of SS genotyped individuals with the 84 bp deletion associated with fatty acid composition and growth traits were very low in both breeds. The frequency of the LL genotype with the 84 bp …
Comparative Diagnostic Methods For Canine Ehrlichiosis, Chetan Parmar, Riddhi Pednekar, Anant Jayraw, Mukulesh Gatne
Comparative Diagnostic Methods For Canine Ehrlichiosis, Chetan Parmar, Riddhi Pednekar, Anant Jayraw, Mukulesh Gatne
Turkish Journal of Veterinary & Animal Sciences
The efficacy of blood and buffy coat smear examination, commercially available dot-enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR) was evaluated in the diagnosis of canine ehrlichiosis in 40 dogs exhibiting symptoms suggestive of the disease. The intracytoplasmic rickettsial organisms were seen in the smears of only 1 dog (2.5%) with an extremely low level of parasitemia. Nested PCR with 16S rRNA revealed characteristic 378 bp bands indicating positive reaction in 8 (20.0%) dogs and sera samples of 21 (52.5%) dogs revealed anti-Ehrlichia antibodies. The dog that revealed organisms in the blood also showed positive results with …
Comparative Evaluation Of Indirect Enzyme Linked Immunosorbent Assay, Rose Bengal Plate Test, Microagglutination Test, And Polymerase Chain Reaction For Diagnosis Of Brucellosis In Buffaloes, Malik Raies Ul Islam, Mohinder Pratap Gupta, Pritam Kaur Sidhu, Gursimran Filia, Hari Mohan Saxena, Tawheed Ahmed Shafi
Comparative Evaluation Of Indirect Enzyme Linked Immunosorbent Assay, Rose Bengal Plate Test, Microagglutination Test, And Polymerase Chain Reaction For Diagnosis Of Brucellosis In Buffaloes, Malik Raies Ul Islam, Mohinder Pratap Gupta, Pritam Kaur Sidhu, Gursimran Filia, Hari Mohan Saxena, Tawheed Ahmed Shafi
Turkish Journal of Veterinary & Animal Sciences
In the present study, 178 blood samples from buffaloes were tested against indirect enzyme linked immunosorbent assay (I-ELISA), rose bengal plate test (RBPT), microagglutination test (MAT), modified microagglutination test (mMAT), and polymerase chain reaction (PCR) to select the most suitable test for efficient and effective diagnosis of bovine brucellosis. Win Episcope 2 software was used to determine the agreement between tests (kappa values at 95% confidence interval). I-ELISA was pair compared with all the other tests. Out of 178 samples, 102 were found positive by I-ELISA, 81 by RBPT, 85 by MAT, 79 by mMAT, and 68 by PCR. Substantial …