Medicine and Health Sciences Commons^™

Metagenomic Analysis Unveils The Microbial Landscape Of Pancreatic Tumors, Sheema Khan, Goutam Banerjee, Saini Setua, Daleniece Jones, Anupam Dhasmana, Bhavin Chauhan, Pratik Banerjee, Murali M. Yallapu, Stephen Behrman, Subhash Chauhan

Research Symposium

Background: The composition of resident microbes in the human body is linked to various diseases and their treatment outcomes. Although studies have identified pancreatic ductal adenocarcinoma (PDAC) associated bacterial communities in the oral and gut samples, herein we hypothesize the prevalence of microbiota in pancreatic tumor tissues is different as compared to their matched adjacent, histologically normal appearing tissues and these microbial molecular signatures can be highly useful for PDAC diagnosis/prognosis.

Methods: In this study, we performed comparative profiling of bacterial populations in pancreatic tumors and their respective adjacent normal tissues using 16S rRNA-based metagenomics analysis.

Results: This study …

Functional Characterization Of A High-Throughput In Vitro Model To Predict Faecal Microbiota Transplantation (Fmt) Donor Success, Catherine M. Andary

Undergraduate Student Research Internships Conference

Faecal microbiota transplantation (FMT) involves the administration of donor faecal matter to a diseased recipient with the goal of remodeling the host microbiome to provide health benefits. In recent years, FMT has emerged as a potential therapy for a variety of microbiome-associated diseases such as atherosclerosis. Trimethylamine (TMA) is an atherosclerosis-linked metabolite generated by the gut microbiota from dietary precursors which is then oxidized to trimethylamine N-oxide (TMAO) by the liver, contributing to increased gut permeability. It has been shown that FMT may alter or restore the gut microbiome of recipients to reduce plasma TMAO levels. Despite its potential, the …

Effect Of Sin Lek Rice Intervention On Cognitive Function, Dietary Patterns, And Fecal Microbiota Of Elementary School Children In Chiang Rai, Thailand, Angie Setthavongsack, Siam Popluechai,, Jackilen Shannon, Justin Denny, Eleni Gentekaki, Kongkiat Kespechara, Lucky Gruneck, Thomas J. Sharpton, Eddie Niwed, Lisa K. Marriott

Student Research Symposium

I will be speaking about our ongoing project with OHSU and Mae Fah Luang University about the effects of a highly fibrous rice called “Sin Lek” and the changes it will do to both the gut microbiome and cognitive function. My data analysis focuses solely on memory, attention span, and impulsivity on Thai schoolchildren. The poster will document baseline data and it’s outcomes, along with brief information about Dr. Siam Popluechai’s lab findings on certain microbiome species. I will also be going in depth about the area of the study, along with how these were tested such as explaining the …

Effect Of Sin Lek Rice Intervention On Cognitive Function, Dietary Patterns, And Fecal Microbiota Of Elementary School Children In Chiang Rai, Thailand, Angie Setthavongsack, Siam Popluechai, Jackilen Shannon, Justin Denny, Eleni Gentekaki, Kongkiat Kespechara, Lucsame Gruneck, Thomas J. Sharpton, Kullawong Niwed, Lisa K. Marriott

OHSU-PSU School of Public Health Annual Conference

Thailand has struggled with malnutrition rates in young children. Rice is a key component of Southeast Asian diets and a whole grain rice varietal (Sin Lek) may support improved nutrition. Diet can influence cognitive function and human gut microbiota, which were explored in the context of a rice intervention. We conducted a pilot study using a non-randomized, crossover clinical trial of Sin Lek rice versus traditional white rice consumption in elementary school children in Chiang Rai, Thailand. Outcomes related to diet, anthropological factors, cognitive function (e.g., short-term working memory, attention, impulsivity), and gut microbial populations were measured at baseline and …

Isolation Of Oral Bacterial Dna For 16s Rrna Sequencing To Determine Nitrate-Sensitive Oral Microbiota Following Acute Beetroot Juice Supplementation, Luka Sharabidze, Leah Stiemsma, Courtney Merrill, Chandler Riley, Maya Hammer, Ryan Kenney, Alyssa Riley, Katie Price, Adam Pennell, Rachel Tan

Seaver College Research And Scholarly Achievement Symposium

Dietary nitrate (NO3-) supplementation, provided as beetroot juice, improves cardiovascular, cognitive, and contractile function by augmenting nitric oxide (NO) biosynthesis; however, the specific taxa responsible, and whether NO3- ingestion can promote a favorable microbial composition to produce NO is yet to be established. To determine whether acute NO3- supplementation is associated with specific microbial taxa in females, 15 healthy adult females consumed an acute dose of NO3--depleted beetroot juice (PL) and NO3--rich beetroot juice (BR). Bacterial DNA from the buccal cells were isolated for subsequent 16s DNA sequencing for preserved regions in their 16s rRNA gene. Concentrations were assessed with …

Recolonization Of Microbiomes’ Influence On Autism Spectrum Disorder, Hattie Cole

Capstone Showcase

In recent years, there has been an increase in the number of studies conducted on the complications of gastrointestinal (GI) comorbidities associated with autism spectrum disorder (ASD) in children. Some of these GI issues include irritable bowel syndrome (IBS), diarrhea and constipation. The causes of these disorders are poorly understood. The dominant gut phyla are Firmicutes and Bacteroidetes, Actinobacteria, Proteobacteria, Fusobacteria and Verrucomicrobia. Research conducted on children with ASD compared with typical developing (TD) children showed higher ratios of Firmicutes/Bacteroidetes in the ASD group. Biflobacterium, Actinobacteria phyla, has been shown to be a good intestinal bacterium that may help with …

Analysis Of The Gut-Brain Axis In Aging: Implications In Alzheimer Disease, Molly Martin, Mary Austin, Denah Appelt

Research Day

The gut microbiome is comprised of the shared genome of the trillions of microorganisms residing in the gastrointestinal ecosystem. The interaction between the host and its gut microbiome, the Gut-Bran Axis (GBA), is a complex relationship whose management could prove critical to preventing or treating not only various gut disorders, like irritable bowel syndrome (IBS) or behavioral health disorders such as general depression and anxiety, but also central nervous system (CNS) disorders, such as Alzheimer's (AD) and Parkinson's (PD) diseases. The purpose of this review is to summarize what is currently known about the gut microbiome, how it is connected …